Solid phase polymerase chain reaction (PCR) kit for chemotherapeutic medication guide gene detection

Abstract

The invention discloses a solid phase polymerase chain reaction (PCR) kit for chemotherapeutic medication guide gene detection, and a detection method, belonging to the technical field of PCR application. The invention also relates to a primer group for detecting ten chemotherapeutic medication guide; the primer group consists of GSTP1 SNP-I105V A313G, MTHFR SNP-C677T, MDR1 G2677AT, XRCC1 SNP-R399Q, CDA G208A, MDR1 SNP-C3435T, CDA A79C, CYP1B1C SNP-4326C / G, ERCC1 SNP-C118T, DPYD IVS14_1G / A; the solid phase PCR amplification kit can be used for detecting the ten gene sites at a time; computer amplification can be realized only by adding a corresponding nucleic acid template into the kit again; the PCR product, obtained after amplification is completed, is subjected to Sanger sequencing; chemotherapeutic medication guide can be realized according to the result of the Sanger sequencing. Compared with the prior art, the solid phase PCR kit adopts a specific primer sequence, so that the reliability of the detection results is ensured; the detection method is simple to operate, time-saving and labor-saving; the solid phase PCR kit is high in detection throughput and low in reagent consumable cost, can be directly used for detecting extracted nucleic acid, is low in requirements for a detection platform and detection personnel, and can be widely popularized in the clinical front line.

Description

technical field [0001] The invention relates to the application field of gene detection, in particular to a solid-phase PCR kit for chemotherapy drug guidance gene detection. Background technique [0002] Tumor is a disease caused by gene mutation. Genetic testing can find out the gene mutation in the tumor. Combined with conventional pathological diagnosis, it can more accurately analyze the type, grade, molecular type, drug sensitivity and other information of the tumor, helping clinicians to have a more accurate diagnosis. Tailored treatment plan. Therefore, please listen carefully to the advice of clinicians, carry out relevant genetic testing under the guidance of doctors, and receive corresponding treatment. [0003] With the development of individualized treatment, more and more clinicians and patients realize that the efficacy and side effects of drugs can be predicted by genetic testing before the treatment of tumors, so as to choose the tumor that suits them, has ...

AI Technical Summary

Problems solved by technology

Lobaplatin is the third-generation platinum-based antineoplastic drug, mainly used for the treatment of breast cancer, small cell lung cancer, and chronic myelogenous leukemia. The adverse reactions include bone marrow suppression, and thrombocytopenia is the most intense, which often occurs 2 weeks after treatment. After 1 week, it returned to 100,000 / mm3, and the blood picture changes were reversible, but secondary side effects such as thrombocytopenia caused bleeding, leukopenia caused a high risk of infection

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