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A polylactic acid/hydroxyapatite/acellular amnion composite scaffold and its construction method

A hydroxyapatite and composite stent technology, which is used in pharmaceutical formulations, prostheses, drug delivery, etc.

Active Publication Date: 2019-06-21
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the combination of acellular amniotic membrane with polylactic acid and hydroxyapatite for bone tissue engineering repair

Method used

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  • A polylactic acid/hydroxyapatite/acellular amnion composite scaffold and its construction method
  • A polylactic acid/hydroxyapatite/acellular amnion composite scaffold and its construction method
  • A polylactic acid/hydroxyapatite/acellular amnion composite scaffold and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of decellularized amniotic membrane and biocompatibility testing

[0034] Place the fresh amniotic membrane in a large petri dish, rinse it repeatedly with normal saline until there is no obvious blood on the surface of the fresh amniotic membrane, use surgical forceps and surgical forceps to find the connection between the amniotic membrane and the chorion, and bluntly peel the amniotic membrane from the chorion. Constantly flush with normal saline to wash away the blood clots mixed with the amniotic membrane. Use surgical scissors to cut the cleaned amniotic membrane into a square of 6 cm×6 cm. The amnion rinsed with PBS was submerged in 3mol / L NaCl solution, and placed in an air bath constant temperature shaking incubator to shake for 30min. After taking it out, rinse it with PBS solution, then add 0.25% trypsin / EDTA digestion solution and shake it at 37°C for 4h. After taking it out, rinse it with PBS solution.

[0035] The biocompatibility ...

Embodiment 2

[0036] Example 2 Preparation of polylactic acid / hydroxyapatite composite scaffold (as a comparative example)

[0037] Weigh 6.0g of polylactic acid (PLA) and add it to 100mL of dichloromethane, stir magnetically in a water bath at 50°C until PLA is completely dissolved in dichloromethane until it becomes clear to obtain a PLA dichloromethane solution; The solution was stirred quickly on a magnetic stirrer, and the n-hexane equivalent to dichloromethane was slowly added dropwise with a dropping funnel. If precipitation occurred, the PLA solution needed to be prepared again. Finally, the solution remained clear after adding n-hexane. , to obtain PLA dichloromethane / n-hexane solution; then add 3.0g hydroxyapatite (nHAp) to the PLA dichloromethane / n-hexane solution, stir rapidly on a magnetic stirrer for premixing, and then in an ultrasonic oscillator Mix further until nHAp is uniformly dispersed in the solution to obtain a PLA / nHAp dichloromethane / n-hexane solution; pour the prep...

Embodiment 3

[0038] Example 3 Preparation of Polylactic Acid / Hydroxyapatite / Acellular Amnion Composite Scaffold

[0039] Weighed 6.0 g of polylactic acid PLA and added it to 100 mL of dichloromethane, and stirred it magnetically in a water bath at 50° C. until the PLA was completely dissolved in the dichloromethane until it became clear to obtain a PLA dichloromethane solution. Quickly stir the PLA dichloromethane solution on a magnetic stirrer, and slowly add n-hexane equivalent to dichloromethane dropwise with a dropping funnel. If precipitation occurs, the PLA solution needs to be prepared again. Still keep the clarification as the criterion, obtain the PLA dichloromethane / n-hexane solution; then add 3.0g hydroxyapatite nHAp to the PLA dichloromethane / n-hexane solution, stir rapidly on a magnetic stirrer for premixing, and then Mix further in the shaker until the nHAp is uniformly dispersed in the solution to obtain a methylene chloride / n-hexane solution of PLA / nHAp; add 2.0 g of decell...

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Abstract

The invention discloses a polylactic acid / hydroxyapatite / acellular amniotic membrane composite stent and a construction method thereof. The polylactic acid / hydroxyapatite / acellular amniotic membrane composite stent is characterized in that polylactic acid, hydroxyapatite / acellular and amniotic membrane are dissolved into dichloroethane and n-hexane by steps, and a non-solvent induced phase separation method is adopted to construct the composite stent which is of a composite pore structure using large pores to coat small pores. The polylactic acid / hydroxyapatite / acellular amniotic membrane composite stent has the advantages that by using the polylactic acid as a substrate, the mechanical strength and biodegradability of the stent are guaranteed; by compounding with the hydroxyapatite, whilethe bone inducing property and bone conduction property of the stent are improved, the acidifying of the metabolism environment is relieved; by compounding with the acellular amniotic membrane, the water-absorbing property, biocompatibility and identification property of the stent are improved, and the sticking and propagation of cells on the stent are promoted; the porosity is high, the pore communication property is good, the mechanical property is strong, the biocompatibility is good, the osteogenesis inducing activity is high, and the like; the good biology function on important seed cells and osteogenesis cells in bone tissue engineering is realized, and the polylactic acid / hydroxyapatite / acellular amniotic membrane composite stent can be used as an excellent biomedical material to be applied to the field of bone tissue engineering.

Description

technical field [0001] The invention belongs to the field of tissue engineering materials, in particular to a tissue engineering scaffold constructed by compounding polylactic acid, hydroxyapatite and decellularized amnion and a construction method thereof. Background technique [0002] Bone tissue engineering, as a new and efficient method for repairing bone tissue defects, has gradually attracted widespread attention in recent years. One of the key steps in the bone tissue engineering repair strategy is to prepare tissue engineering scaffolds that are nontoxic, metabolizable, biocompatible, osteoinductive, and have suitable mechanical strength. However, almost no single material can meet all the above requirements at the same time. Therefore, it is necessary to combine different types of materials with different properties to synergistically exert the advantages and functions of each material to meet the needs of bone repair and reconstruction. [0003] Polylactic acid (P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/36A61L27/56A61L27/58
CPCA61L27/3604A61L27/3687A61L27/46A61L27/56A61L27/58A61L2400/18A61L2430/02C08L67/04
Inventor 宋克东闫新宇卢延国李丽颖李文芳刘天庆
Owner DALIAN UNIV OF TECH
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