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Application of Bacillus amyloliquefaciens in the control of plant fungal diseases

A technology for plant fungal diseases and amylolytic spores, which is applied in the application field of Bacillus amyloliquefaciens in the prevention and treatment of plant fungal diseases, to achieve safe and stable biological control effects

Active Publication Date: 2020-04-03
广州市林业和园林科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the application of endophytic bacteria to control fungal diseases such as tree brown root disease, and the corresponding selection of bacteria and their antagonistic bacteria is also an important factor and index for the selection of endophytic bacteria for biocontrol

Method used

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  • Application of Bacillus amyloliquefaciens in the control of plant fungal diseases
  • Application of Bacillus amyloliquefaciens in the control of plant fungal diseases
  • Application of Bacillus amyloliquefaciens in the control of plant fungal diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Isolation and identification of Bacillus amyloliquefaciens strain BSY3

[0034] 1. Isolate strains from marigold plants collected in Guangzhou Baiyun Nursery. The specific isolation method is as follows:

[0035] (1) Single colony isolation

[0036] 1) Clean the collected fresh marigold leaf material with tap water;

[0037] 2) After rinsing once with sterile water, soak in 75% alcohol for 5 minutes, soak in 2% sodium hypochlorite solution for 2 minutes, and then rinse with sterile water 4 times, and spread the sterile water after the last rinse as a blank control ;

[0038] 3) Put the sterilized fresh plant material in a sterilized mortar, add a small amount of sterile water to grind it into a homogenate, and then dilute it into different gradients of the grinding solution and spread it on a flat plate;

[0039] 4) Place the plate in a 30°C incubator for culture, observe the growth of the colonies, then pick colonies of different shapes, purify and cultiva...

Embodiment 2

[0059] Example 2 Optimum culture condition optimization of Bacillus amyloliquefaciens strain BSY3

[0060] 1. The most suitable medium for strain growth

[0061] (1) Optimization method: Strain BSY3 was streaked and activated on LB medium (30°C), then inoculated into LB liquid medium, and activated at 280 rpm and 30°C. Inoculate the activated strain BSY3 with 0.5% inoculum in the shake flask fermentation basal medium YGN [yeast extract 10g (when different nitrogen sources), glucose 10 g (when different carbon sources), NaCl 5 g (when different inorganic salts ), dilute to 1 L, pH 7.0]. After inoculation, culture in a shaker at 30°C and 160 rpm for 2 to 5 days, and then centrifuge the fermentation broth of the above-mentioned different substances (carbon source, nitrogen source, and inorganic salt) at 12,000 rpm for 15 min, and the supernatant was filtered through a 0.22 μm microsphere. The original fermentation liquid is obtained after filtration by the porous filter membran...

Embodiment 3

[0075] Example 3 Detection of the antagonistic effect of Bacillus amyloliquefaciens strain BSY3 on common pathogenic fungi

[0076] 1. The BSY3 strain was co-cultured with several pathogenic fungi, and its antagonism to pathogenic fungi was detected.

[0077] Tested strains: Harmful Phylonotus xylinum ( P. noxius ), white butterfly anthracnose pathogen ( Colletotrichum sp.), canna blast pathogen ( Pyricularia cannaecola ), black pine leaf spot pathogen ( Pestalotiopsis sp.), the pathogen of banana wilt ( Fusarium oxysporum f. sp. cubes ) and Sclerotinia sclerotiorum pathogen ( Sclerotinia sclerotiorum ).

[0078] 2. Persistence culture detection method:

[0079] (1) Inoculate a 1% (v / v) ratio of the bacterial solution into a 150 mL Erlenmeyer flask filled with 40 mL LB medium, and incubate at 28°C and 200 rpm for 2.5 days;

[0080] (2) Centrifuge at 8000 rpm for 10 min to collect the supernatant, and then pass through a 0.22 μm filter membrane to obtain the cru...

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Abstract

The invention discloses the application of the amylobacter bacillus in the prevention and treatment of plant fungal diseases. The present invention has found that Bacillus amyloliquefaciens, especially the Bacillus amyloliquefaciens strain BSY3 isolated and screened by the inventor from marigolds, is effective against brown root bacteria, canna blast fungus, rape sclerotinia, white butterfly anthracnose bacteria, black pine Common plant fungi such as Leaf Spot and Banana Fusarium wilt have obvious antagonistic effects, and have significant biocontrol effects and potential application values ​​in the prevention and treatment of fungal diseases caused by these pathogens. Moreover, Bacillus amyloliquefaciens is an endophytic bacterium derived from plants. Compared with traditional chemical control methods, it has a safer biocontrol effect on fungal diseases; and it has been in plants for a long time and can establish harmonious coexistence with plants. , The survival relationship of stable development, compared with chemical agents, can play its biological control function more effectively and stably.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. More specifically, it relates to the application of Bacillus amyloliquefaciens in the prevention and treatment of plant fungal diseases. Background technique [0002] Tree fungal diseases are forest diseases caused by pathogenic fungi, such as brown root disease, which are caused by harmful Phylopores ( Phellinus noxius ) is a soil-borne disease that causes devastating damage to commercial crops, fruit trees, ancient and famous trees, and landscaping plants. It is known as "tree cancer". At present, prevention and control measures such as cutting down diseased trees, digging ditches to block the spread of pathogens, fumigation of diseased soil, and planting disease-free seedlings are mainly taken, but there is no ideal control agent and control method that can effectively control the occurrence of tree brown root disease. . In addition, although there are currently som...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/22A01P3/00C12N1/20C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 孙龙华黄华枝阮琳毕可可邓嘉茹张劲蔼吴超唐立鸿胡彦辉邓从双候亚会
Owner 广州市林业和园林科学研究院
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