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Methyl nutritional type bacillus B18, and granules and application thereof

A methylotrophic and Bacillus technology, applied in the field of microorganisms, can solve the problems of easy residue, human and animal safety, environmentally friendly biological control not involved, and large environmental pollution of chemical pesticides, etc., to achieve good antagonism, good control effect, The effect of low cost

Active Publication Date: 2018-03-06
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, soil disinfection, silvicultural measures, and chemical control are the main means of prevention and control of the disease, but chemical pesticides cause serious environmental pollution and are easy to remain; biological control that is safe for humans and animals and environmentally friendly has not involved

Method used

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  • Methyl nutritional type bacillus B18, and granules and application thereof
  • Methyl nutritional type bacillus B18, and granules and application thereof
  • Methyl nutritional type bacillus B18, and granules and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and Identification of Methylotrophic Bacillus B18

[0030] 1.1 Isolation of methylotrophic Bacillus B18

[0031] Healthy Phellodendron bark was collected from the area where Phellodendron rotten skin disease occurred in Yangtianwo Pharmacy, Dayi, Sichuan. Rinse the collected samples with sterile water, weigh 1.0g of tissue, soak in 70% alcohol for 1-2min, then disinfect with 1-3% sodium hypochlorite solution for 3-5min, wash several times with sterile water, absorb Apply 100 μL of washing liquid for the last time to NA plate (3g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar powder, 1000mL distilled water, pH 7.0, after mixing and dispensing, autoclave at 121°C for 30min), culture in the dark at 27°C 24h, used as a control for surface disinfection, to check whether the surface disinfection is thorough.

[0032] Cut the surface-sterilized bark tissue into pieces, put them into a sterile mortar, add sterilized quartz sand and 10mL of ster...

Embodiment 2

[0047] The making of embodiment 2 methylotrophic bacillus B18 granules

[0048] Nutritious succulent culture solution (beef extract 7.0g, peptone 10g, NaCL 5g, water 1000mL) is autoclaved, bottled according to the proportion of 100mL liquid in a 300mL triangular flask (oxygen control), and inoculated with B18 slant seeds in a sterile state , each bottle was inoculated with 2 slant seeds, the temperature was 27°C, the initial pH value was 7.0, and the culture was shaken at 120r / min for 48h to prepare B18 fermentation broth.

[0049] Liquid fermentation medium (glycerin 5g, dextrin 5g, soybean cake powder 5g, ammonium oxalate 10g, sodium chloride 2g, calcium carbonate 3g, water 1000mL) after autoclaving, under the control of ventilation, put it in 200mL liquid Proportional bottling in 500mL triangular flasks, inoculation of secondary liquid seeds of methylotrophic Bacillus in a sterile state with an inoculation volume of 10%, temperature at 27°C, initial pH value of 7.0, 120r / mi...

Embodiment 3

[0053] Embodiment 3 potted control effect experiment

[0054] The annual potted Eucommia ulmoides seedlings were selected as the experimental object, and the conidia of Pythium debaryanum (Pythium debaryanum) on the PDA slope (spore concentration of 2×10 6 cfu / mL) 50mL) 50mL inoculates the root system one week later, adopts the granule that embodiment 2 makes to adopt the spreading method to divide different dosages to treat Eucommia seedlings, every pot 25, 50, 75, 100, 125, 150g, every 7 days 1 time (total 3 times), and use sterile water as a control, repeat 3 times, and count the incidence of Eucommia ulmoides. There were 10 Eucommia seedlings per treatment.

[0055] After 30 days of inoculation with pathogenic bacteria, conduct disease investigation and statistics according to the disease grading standards in Table 3, and calculate the incidence rate, disease index and control effect.

[0056] Table 3 Grading standards for root rot disease of Eucommia ulmoides

[0057] ...

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Abstract

The invention discloses methyl nutritional type bacillus B18, and granules and application thereof. The strain was preserved into China General Microbiological Culture Collection Center on September 18, 2017, and the preservation number is CGMCC No.14641. The invention also provides methyl nutritional type bacillus B18 granules. The methyl nutritional type bacillus B18 granules comprise the following components in percentage by weight: 10 percent of seed fermenting liquid, 15 percent of a solid fermenting preparation, 15 percent of an adhesive and the balance of carrier, wherein the total amount is 100 percent. The methyl nutritional type bacillus B18 and the granules thereof have special effect on eucommia root rot, is suitable for commercial production, has high environmental compatibility and has the characteristics of sustainability and low cost.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a methylotrophic bacillus B18 strain and its granule and its application in the prevention and treatment of eucommia root rot. Background technique [0002] Eucommia ulmoides belongs to the Eucommia genus Eucommia perennial deciduous tree. There is only one genus and one species in this family. It is the only tertiary relic plant in my country. Eucommia has a cultivation history of nearly a thousand years and is extremely practical. It can be grown in 27 provinces from subtropical to temperate regions in my country, mainly in Sichuan, Yunnan, Guizhou, Hunan, Hubei, Shaanxi, Jiangsu and Zhejiang provinces. Eucommia ulmoides is listed as one of the demonstration varieties of Guizhou authentic Chinese medicinal materials GAP base. It is full of treasures, has a wide range of uses, and has great medicinal and economic value. Eucommia ulmoides is known as a magical tree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 李姝江崔玉婷朱天辉方馨玫黎肇家谯天敏韩珊
Owner SICHUAN AGRI UNIV
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