Novel dihydrogen bond deep-eutectic solvent and method for pretreating rice straws through combination of solvent and sodium carbonate

A deep eutectic solvent and pretreatment technology, applied in the field of chemical engineering, can solve the problems of high price, low toxicity and biodegradability, and achieve the effect of low cost, low cost and simple synthesis

Inactive Publication Date: 2018-03-06
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the "green aspect" of ionic liquids has recently been greatly questioned, their possible toxicity and very low biodegradability are currently reported in the literature, and they are expensive

Method used

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  • Novel dihydrogen bond deep-eutectic solvent and method for pretreating rice straws through combination of solvent and sodium carbonate
  • Novel dihydrogen bond deep-eutectic solvent and method for pretreating rice straws through combination of solvent and sodium carbonate
  • Novel dihydrogen bond deep-eutectic solvent and method for pretreating rice straws through combination of solvent and sodium carbonate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 The synthesis of double hydrogen bond deep eutectic solvent

[0015] Accurately weigh 100g of different molar ratios of choline chloride: formic acid: acetic acid (1:0.5:0.5~2:1.5:1.5) into a three-neck flask for mixing, and stir the reaction at 60°C for 2 hours. At this time, a uniform, clear and transparent of liquid. The liquid is transferred into a round-bottomed flask, and incompletely reacted formic acid and acetic acid are removed by distillation under reduced pressure, finally obtaining choline chloride of different proportions: formic acid: acetic acid deep eutectic solvent. 10 g of rice straw was weighed and added to the solvent, stirred at 140° C. for 2 h, then the mixture was centrifuged, the precipitate was washed with deionized water three times, and dried to a constant weight. Weigh 1g of the treated straw, add 10mL pH 4.8, 50mM citric acid-sodium citrate buffer, continue to add 50FPU cellulase ( 1500), 50° C. and 150 rpm for 24 h on a sha...

Embodiment 2

[0018] Example 2 Double hydrogen bond deep eutectic solvent pretreatment of rice straw

[0019] Measure 100g of choline chloride: formic acid: acetic acid deep eutectic solvent to a three-necked flask, weigh 5 to 20g of rice straw (the mass ratio of solvent to rice straw is 5:1 to 20:1) and add it to the solvent, Stir at 140°C for 0.5-3 hours, then centrifuge the mixture, wash the precipitate with deionized water three times, and dry to constant weight. Weigh 1g of the treated straw, add 10mL pH4.8, 50mM citric acid-sodium citrate buffer, continue to add 30-70FPU cellulase ( 1500), 50° C. and 150 rpm for 24 h on a shaker in a water bath. Glucose, xylose and arabinose in the hydrolyzate were determined by high performance liquid chromatography, as shown in Table 2. During the pretreatment process, with the increase of straw addition, the content of total sugar after pretreatment gradually increased. In view of the highest utilization efficiency of ionic liquid and straw, 10...

Embodiment 3

[0023] Example 3 Combined double hydrogen bond deep eutectic solvent and sodium carbonate pretreatment of rice straw

[0024] Accurately weigh 10 g of the rice straw pretreated by the double hydrogen bond deep eutectic solvent in Example 2, add 0-10% sodium carbonate solution, treat at 60-140°C for 0.5-3h, then centrifuge the mixture, and use The precipitate was washed three times with deionized water and dried to constant weight. Weigh 1g of the treated straw, add 10mL pH 4.8, 50mM citric acid-sodium citrate buffer, continue to add 50FPU cellulase ( 1500), 50° C. and 150 rpm for 24 h on a shaker in a water bath. Glucose, xylose and arabinose in the hydrolyzate were determined by high performance liquid chromatography, as shown in Table 3. The total sugar content of 0.5% sodium carbonate solution after pretreatment at 140°C for 1 hour has reached 43.13g / L. In view of the environmental protection of the pretreatment reagent, 0.5% sodium carbonate was selected as the combined ...

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Abstract

The invention discloses a novel dihydrogen bond deep-eutectic solvent and a method for pretreating rice straws through combination of the solvent and sodium carbonate; straw hydrolysate after enzymolysis can be used for clostridium fermentation to synthesize bio-butanol. The deep-eutectic solvent provided by the invention is the novel dihydrogen bond deep-eutectic solvent which is synthesized by stirring choline chloride, formic acid and acetic acid at a molar ratio of 1:1:1 at the temperature of 30 DEG C; the solvent can be used for reducing the contents of lignin and hemicellulose in a lignocellulose raw material, so that the accessibility of cellulose in the lignocellulose raw material. The solvent and the sodium carbonate are combined to pretreat the rice straws, and enzyme hydrolysisis performed on the pretreated rice straws with cellulose, so as to obtain the hydrolysate containing 4.28g / L of total reducing sugar; the yield of glucose is 80%; in addition, the hydrolysate has noobvious inhibiting effect on the growth of microorganisms such as clostridium and the like, and the yield of butanol is 13.73g.L<-1>. The dihydrogen bond deep-eutectic solvent is an environment-friendly solvent which is simple in synthesis and convenient to use and has good application and development prospects in pretreatment of the lignocellulose raw material.

Description

technical field [0001] The invention discloses a novel double hydrogen bond deep eutectic solvent and a method for pretreating rice straw and hydrolyzate to ferment butanol by using it in combination with sodium carbonate, and belongs to the field of chemical engineering. Background technique [0002] Today's world has an increasing demand for non-renewable energy sources such as oil, natural gas and coal. The oil crisis in the 1970s has aroused the general concern of countries all over the world on the problem of future energy shortage. Traditional coal, oil and natural gas are all non-renewable energy sources, and the development of remaining reserves is becoming more and more difficult. In addition, my country is a large agricultural country, and the raw materials of lignocellulose are quite abundant. Crop straw is rich in cellulose and hemicellulose and is a valuable renewable resource. However, a considerable amount of crop stalks are discarded or burned, which not o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/16C12R1/145
CPCC12P7/16C12P19/02C12P19/14C12P2201/00C12P2203/00Y02E50/10
Inventor 倪晔许国超邢婉茹董晋军
Owner JIANGNAN UNIV
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