Hemostatic anti-adhesion film and preparation method thereof
An anti-adhesion film and sodium hyaluronate technology, applied in the field of medical surgery, can solve the problems of cross-linking agent residue product biocompatibility research, good biocompatibility and tissue adhesion, and deposition in other organs of the human body , to prevent damage to the structure of collagen and polysaccharides, ensure biocompatibility, and excellent biocompatibility
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[0036] In the second aspect, the present invention provides a method for preparing a hemostatic and anti-adhesion film, comprising: uniformly mixing the freeze-dried collagen powder and sodium hyaluronate according to the above weight ratio to obtain a mixture; adding a cross-linking agent to the mixture for cross-linking joint reaction to obtain a blended solution; and drying the blended solution, pressing it into a membrane and sterilizing it.
[0037] Specifically, the preparation method of the present invention comprises the following steps:
[0038] In the first step, collagen is mixed with sodium hyaluronate.
[0039] As mentioned above, the collagen is preferably recombinant human collagen obtained by microbial fermentation, and the recombinant human collagen freeze-dried powder is obtained after filter sterilization and freeze-drying. As mentioned above, the sodium hyaluronate is preferably pharmaceutical grade sodium hyaluronate. When mixing, first dissolve pharmace...
Embodiment 1
[0053] (1) Dissolve 2.5 grams of pharmaceutical grade sodium hyaluronate with a molecular weight of 180Kda in purified water, and then add 10 grams of recombinant human collagen freeze-dried powder with a molecular weight of 70Kda, the concentration of recombinant human collagen is 0.8%, The concentration of sodium hyaluronate is 0.2%, stir evenly to obtain a mixture;
[0054] (2) Add glutaraldehyde to the above mixture for the first cross-linking reaction, the final concentration of glutaraldehyde is 0.03mol / L, the reaction temperature is 20°C, and the time is 4h; then add divinyl sulfone for the second cross-linking reaction Reaction, the final concentration of divinyl sulfone is 0.03mol / L, the reaction temperature is 20°C, the time is 6h, PBS is repeatedly washed to remove the remaining cross-linking agent, and a blended solution is obtained;
[0055] (3) Pack the above blended solution in a mold, pre-freeze, freeze-dry and press to form a film, then cut the film into 6cm×8...
Embodiment 2
[0057] (1) Dissolve 5 grams of pharmaceutical grade sodium hyaluronate with a molecular weight of 150Kda in purified water, then add 10 grams of recombinant human collagen freeze-dried powder with a molecular weight of 40Kda, the concentration of recombinant human collagen is 0.4%, The concentration of sodium hyaluronate is 0.2%, stir evenly to obtain a mixture;
[0058] (2) Add carbodiimide to the above mixture for the first cross-linking reaction, the final concentration of carbodiimide is 0.02mol / L, the reaction temperature is 4°C, and the time is 2h; then continue to add 1,4 butanediol di The glycidyl ether was subjected to the second cross-linking reaction, the final concentration of 1,4-butanediol diglycidyl ether was 0.05mol / L, the reaction temperature was 4°C, the time was 5h, and the remaining cross-linking agent was repeatedly washed with PBS to obtain a blend solution;
[0059] (3) Divide the above blended solution into molds, pre-freeze, freeze-dry and press to fo...
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