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Hemostatic anti-adhesion film and preparation method thereof

An anti-adhesion film and sodium hyaluronate technology, applied in the field of medical surgery, can solve the problems of cross-linking agent residue product biocompatibility research, good biocompatibility and tissue adhesion, and deposition in other organs of the human body , to prevent damage to the structure of collagen and polysaccharides, ensure biocompatibility, and excellent biocompatibility

Active Publication Date: 2021-03-30
SHAANXI HUIKANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The Chinese invention patent application with the application number 200910107412.4 discloses a water-soluble hemostatic material, the main component is oxidized regenerated cellulose ether, which has a remarkable hemostatic effect, but there is a problem of insufficient strength, and it is difficult to degrade in the body, and may be deposited in other parts of the human body after absorption. in the organ
[0006] The Chinese invention patent application with the application number 201310380614.2 discloses an anti-adhesion hemostatic film, the main component is dextran, which is prepared by electrospinning technology after cross-linking. The production process is relatively complicated, the cost is high, and there is no cross-linking agent for the product. Research on residues and product biocompatibility
[0007] The materials disclosed in the above documents all have certain deficiencies. They cannot simultaneously possess the properties of hemostasis, anti-adhesion, biodegradability, biocompatibility and good tissue adhesion, and cannot well meet the needs of the current surgical field.

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  • Hemostatic anti-adhesion film and preparation method thereof
  • Hemostatic anti-adhesion film and preparation method thereof

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preparation example Construction

[0036] In the second aspect, the present invention provides a method for preparing a hemostatic and anti-adhesion film, comprising: uniformly mixing the freeze-dried collagen powder and sodium hyaluronate according to the above weight ratio to obtain a mixture; adding a cross-linking agent to the mixture for cross-linking joint reaction to obtain a blended solution; and drying the blended solution, pressing it into a membrane and sterilizing it.

[0037] Specifically, the preparation method of the present invention comprises the following steps:

[0038] In the first step, collagen is mixed with sodium hyaluronate.

[0039] As mentioned above, the collagen is preferably recombinant human collagen obtained by microbial fermentation, and the recombinant human collagen freeze-dried powder is obtained after filter sterilization and freeze-drying. As mentioned above, the sodium hyaluronate is preferably pharmaceutical grade sodium hyaluronate. When mixing, first dissolve pharmace...

Embodiment 1

[0053] (1) Dissolve 2.5 grams of pharmaceutical grade sodium hyaluronate with a molecular weight of 180Kda in purified water, and then add 10 grams of recombinant human collagen freeze-dried powder with a molecular weight of 70Kda, the concentration of recombinant human collagen is 0.8%, The concentration of sodium hyaluronate is 0.2%, stir evenly to obtain a mixture;

[0054] (2) Add glutaraldehyde to the above mixture for the first cross-linking reaction, the final concentration of glutaraldehyde is 0.03mol / L, the reaction temperature is 20°C, and the time is 4h; then add divinyl sulfone for the second cross-linking reaction Reaction, the final concentration of divinyl sulfone is 0.03mol / L, the reaction temperature is 20°C, the time is 6h, PBS is repeatedly washed to remove the remaining cross-linking agent, and a blended solution is obtained;

[0055] (3) Pack the above blended solution in a mold, pre-freeze, freeze-dry and press to form a film, then cut the film into 6cm×8...

Embodiment 2

[0057] (1) Dissolve 5 grams of pharmaceutical grade sodium hyaluronate with a molecular weight of 150Kda in purified water, then add 10 grams of recombinant human collagen freeze-dried powder with a molecular weight of 40Kda, the concentration of recombinant human collagen is 0.4%, The concentration of sodium hyaluronate is 0.2%, stir evenly to obtain a mixture;

[0058] (2) Add carbodiimide to the above mixture for the first cross-linking reaction, the final concentration of carbodiimide is 0.02mol / L, the reaction temperature is 4°C, and the time is 2h; then continue to add 1,4 butanediol di The glycidyl ether was subjected to the second cross-linking reaction, the final concentration of 1,4-butanediol diglycidyl ether was 0.05mol / L, the reaction temperature was 4°C, the time was 5h, and the remaining cross-linking agent was repeatedly washed with PBS to obtain a blend solution;

[0059] (3) Divide the above blended solution into molds, pre-freeze, freeze-dry and press to fo...

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Abstract

The invention provides an anti-adhesion hemostatic membrane which comprises collagen and sodium hyaluronate and a preparation method of the anti-adhesion hemostatic membrane. The preparation method comprises the following steps: freeze-dried collagen powder and sodium hyaluronate are mixed uniformly, and a mixture is obtained; a crosslinking agent is added to the mixture, a crosslinking reaction is performed, and a blended solution is obtained; the blended solution is dried and pressed into the membrane, and the membrane is sterilized. The anti-adhesion hemostatic membrane has the anti-adhesion and hemostatic effects simultaneously, is free of immunogenicity, has no risk of animal derived infectious disease, is high in water absorption and hemostatic amino acid sequence content, and can becompletely degraded in vivo.

Description

technical field [0001] The invention belongs to the technical field of medical surgery, in particular, the invention relates to a biodegradable hemostatic and anti-adhesion film and a preparation method thereof. Background technique [0002] In the medical field, hemostatic materials and products are widely used in clinical practice. Especially in surgical departments, a considerable number of traumatic deaths are caused by excessive acute bleeding. Therefore, rapid hemostasis and effective control of blood loss are the key to saving lives. , in many surgical procedures, the control of bleeding points of various wounds is also an important factor to reduce complications and improve the success of surgery. In addition to bleeding control in surgery, another issue worthy of attention is postoperative adhesion. As one of the serious postoperative complications, postoperative adhesion brings great pain and suffering to patients. Therefore a kind of product with superior hemosta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/04A61L24/00A61L31/04A61L31/14A61L31/16
CPCA61L24/0015A61L24/0042A61L24/043A61L31/041A61L31/148A61L31/16A61L2300/236A61L2300/252A61L2400/04C08L89/00C08L5/08
Inventor 何越
Owner SHAANXI HUIKANG BIO TECH CO LTD
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