Extraction and purification method of polysaccharide from Polygonatum kingianum
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A purification method, the technology of Polygonatum yunnanensis, applied in the field of purification, can solve the problems of complex operation, high cost, low repeatability, etc., and achieve the effect of improving economic benefits
Inactive Publication Date: 2018-03-23
DALI UNIV
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At present, the methods of deproteinization mainly include proteolysis method, sevage method, trichloroacetic acid method, etc., which are complicated to operate and use organic solvents; the methods of removing pigment from plant polysaccharides include activated carbon decolorization, cellulose anion exchange resin decolorization, etc., most of which are effective for polysaccharides. Destructive effect; commonly used methods for removing small molecules include ultrafiltration, gel filtration, etc., with high cost and low repeatability
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[0023] Step 1, degreasing treatment of Polygonatum Polysaccharides
[0024] Accurately weigh an appropriate amount of dry Rhizoma Polygonatum medicinal material coarse powder, carry out reflux degreasing for 2 hours according to m (medicinal material): m (95% ethanol) = 1:2, filter to obtain slag, evaporate the solvent, and set aside.
[0025] Step 2. Orthogonal test of polysaccharide extraction from Polygonatum Polygonatum
[0026] Select decoction time, liquid-to-material ratio (the mass ratio of used water to medicinal materials), decoction times as investigation factors, carry out L9(3 4 ), the level design of each factor is shown in Table 1.
[0027] Table 1 Factors and levels of an orthogonal design
[0028]
[0029] Accurately weigh 9 parts of Polygonatum Polysaccharides crude polysaccharide after degreasing, every part of 10g, press L9 (3 4 ) conditions given in the table to extract, the combined filtrate, the filtrate was concentrated under reduced pressure at 6...
Embodiment 1
[0041] S1. Take 200 g of Rhizoma Polygonatum powder passed through a No. 3 sieve, add 400 g of 95% ethanol for reflux degreasing for 2 hours, filter to obtain slag, evaporate the solvent, and set aside.
[0042] S2. Add appropriate amount of water to the degreased Polygonatum Polygonatum powder according to the liquid-to-solid ratio of 10:1, and extract 3 times for 40 minutes each time. Combine the filtrates, concentrate under reduced pressure at 60°C to 1:1.5 (the amount of the original medicinal material: the amount of the medicinal solution), add 95% ethanol, stir evenly, carry out alcohol precipitation at room temperature with a final ethanol concentration of 70%, and collect the precipitate after 24 hours; The supernatant after alcohol precipitation was concentrated under reduced pressure to 1.0 times the volume of the original medicinal material, then 95% ethanol was added, and alcohol precipitation was carried out at room temperature with a final concentration of ethanol...
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Abstract
The invention discloses an extraction and purification method of polysaccharide from Polygonatum kingianum, comprising the following steps: degreasing crude powder of a Polygonatum kingianum medicinalmaterial, carrying out water extraction for three times (40 min for each time), mixing filtrates, carrying out reduced pressure concentration at 60 DEG C to 1: 1.5, and carrying out alcohol precipitation for two times; mixing precipitates, and freeze-drying to obtain crude polysaccharide from Polygonatum kingianum; preparing a 0.3-0.5 g/mL solution by the use of the crude polysaccharide from Polygonatum kingianum, and carrying out depigmentation by loading the solution into D-101 macroporous resin; removing protein in the crude polysaccharide from Polygonatum kingianum by an isoelectric pointmethod; and carrying out dialysis by placing the protein-removed crude polysaccharide solution into a dialysis bag. By the method of the invention, polysaccharide in Polygonatum kingianum can be extracted effectively, impurities such as pigment, protein, micromolecular substances and the like in polysaccharide can be more thoroughly removed, and the structure of polysaccharide will not be damaged. In addition, use amount of an organic reagent is reduced, and polysaccharide purity is raised. The invention is an innovation of a purification method of polysaccharide from Polygonatum kingianum.
Description
technical field [0001] The invention relates to the technical field of purification, in particular to a method for extracting and purifying Polygonatum Polysaccharides. Background technique [0002] Polygonatum kingianum Coll.et Hemsl is a perennial herb of Liliaceae Polygonatum kingianum Coll.et Hemsl. It has the effects of invigorating qi and nourishing yin, invigorating the spleen, moistening the lungs and nourishing the kidney. For spleen and stomach qi deficiency, body fatigue and fatigue, insufficient stomach yin, dry mouth, lack of food, lung deficiency and dry cough embolism. It is mainly produced in Yunnan, commonly known as "Rhubarb Rhizoma", and has better quality. It is an important authentic medicinal material in Yunnan. [0003] The main components of Polygonatum Polygonatum are polysaccharides, saponins, flavonoids, vitamins, and trace elements. Modern phytochemical and pharmacological experiments have proved that Polygonatum polysaccharide is one of the ma...
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