A kind of biological fungicide and preparation method thereof

A kind of antifungal agent and biological technology, applied in the field of antibacterial, can solve the problems of expensive equipment, cumbersome operation, drug residue and other problems of physical control technology, and achieve the effect of fast reproduction speed, simple operation and high feasibility

Active Publication Date: 2020-05-12
YANGZHOU UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two methods have great disadvantages. The equipment of physical prevention technology is expensive and the operation is cumbersome, and people are also worried that radiation will be harmful to the human body; problems such as drug residues caused by chemical antiseptic technology are becoming more and more worrying.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of biological fungicide and preparation method thereof
  • A kind of biological fungicide and preparation method thereof
  • A kind of biological fungicide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of preparation method of biological fungicide of the present invention, comprises the steps:

[0041] (1) Preparation of B.amyloliquefaciens F1 fermentation broth

[0042]The B. amyloliquefaciens F1 strain was inoculated into a test tube containing 5 mL of nutrient broth at a 2% inoculation amount, and cultured on a shaker at 30° C. to obtain a B. amyloliquefaciens F1 seed solution. Aspirate B.amyloliquefaciens F1 seed liquid, add 2% inoculum amount to an Erlenmeyer flask with 40% fermentation medium loading, culture at 30°C on a shaking table for 48 hours, and obtain B.amyloliquefaciens F1 fermentation liquid.

[0043] (2) Preparation of B.amyloliquefaciens F1 crude antibacterial protein

[0044] First, take 1 L of the above-mentioned B. amyloliquefaciens F1 fermentation broth and perform ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 15 kDa to obtain an ultrafiltration concentrate. Then slowly add ammonium sulfate to the u...

Embodiment 2

[0049] A kind of preparation method of biological fungicide of the present invention, comprises the steps:

[0050] (1) Preparation of B.amyloliquefaciens F1 fermentation broth

[0051] The B. amyloliquefaciens F1 strain was inoculated into a test tube containing 5 mL of nutrient broth at a 2% inoculation amount, and cultured on a shaker at 30° C. to obtain a B. amyloliquefaciens F1 seed liquid. Aspirate B.amyloliquefaciens F1 seed liquid, add 2% inoculum amount to an Erlenmeyer flask with 20% fermentation medium loading, and culture on a shaker at 32°C for 72 hours to obtain B.amyloliquefaciens F1 fermentation liquid.

[0052] (2) Preparation of B.amyloliquefaciens F1 crude antibacterial protein

[0053] First, take 1 L of the above-mentioned B. amyloliquefaciens F1 fermentation broth and perform ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 15 kDa to obtain an ultrafiltration concentrate. Then ammonium sulfate was slowly added to the u...

Embodiment 3

[0060] A kind of preparation method of biological fungicide of the present invention, comprises the steps:

[0061] (1) Preparation of B.amyloliquefaciens F1 fermentation broth

[0062] The B. amyloliquefaciens F1 strain was inoculated into a test tube containing 5 mL of nutrient broth at a 2% inoculation amount, and cultured on a shaker at 30° C. to obtain a B. amyloliquefaciens F1 seed solution. Aspirate B.amyloliquefaciens F1 seed solution, add 2% inoculum amount to an Erlenmeyer flask with 30% loading capacity of fermentation medium, and culture on a shaker at 37°C for 70 hours to obtain B.amyloliquefaciens F1 fermentation solution.

[0063] (2) Preparation of B.amyloliquefaciens F1 crude antibacterial protein

[0064] First, take 1 L of the above-mentioned B. amyloliquefaciens F1 fermentation broth and perform ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 8 kDa to obtain an ultrafiltration concentrate. Then ammonium sulfate was slow...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a biological mildewproof agent and a preparation method thereof. The biological mildewproof agent comprises the following raw materials in terms of 100 parts of water by weight: 2 to 8 parts of antibacterial protein or a crude antibacterial protein product, 0.5 to 3 parts of a film forming agent, 0.5 to 5 parts of a plasticizer and 0.5 to 4 parts of a cross-linking agent. The preparation method comprises the following steps: adding water into the film forming agent, completely dissolving the film forming agent, then adding the antibacterial protein, the plasticizer andthe cross-linking agent, and carrying out uniform mixing so as to obtain a biological mildewproof solution. According to the invention, the crude antibacterial protein product is a fermentation brothof B.amyloliquefaciens F1 and is obtained by ultrafiltration and ammonium sulfate fractional precipitation, while the antibacterial protein is prepared from the above-mentioned crude antibacterial protein product through further separation means like dialysis, ion exchange and gel chromatography. The biological mildewproof solution obtained by adding a main antibacterial substance namely the antibacterial protein inhibits the growth of Aspergillus flavus, has characteristics like safety and high efficiency, and solves the problems of high price and complex operation for physical preservation and a problem of pesticide residue brought by chemical preservation. The biological mildewproof agent provided by the invention has the advantages of economy, high efficiency, convenient use, and greatapplication prospects in the fields of agriculture and food preservation.

Description

technical field [0001] The invention relates to the field of antibacterial technology, in particular to a preparation method of a biological antifungal agent. Background technique [0002] Aspergillus flavus belongs to the half-knowledge fungus, the colony grows rapidly, the surface is yellow-green, and the back is slightly reddish brown. Thale growth usually presents many branched hyphae, forming mycelium. Once the mycelium is formed, it secretes degradative enzymes or proteins to break down complex nutrients. Individual hyphae are usually invisible to the naked eye, but the thick hyphae mats produced by the conidia can be easily seen. Aflatoxin, a secondary metabolite produced by Aspergillus flavus, belongs to a large class of mycotoxins and is widely found in various grains (corn, rice), oil and oil crops (peanut oil, peanuts, nuts), fruits and vegetables, milk and In meat, it has teratogenic and carcinogenic hazards. [0003] There are physical methods, chemical meth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/22A01N37/46A01P1/00A01P3/00C12P21/00C07K1/36C07K1/34C07K1/18C07K1/14C12R1/07
CPCA01N37/46A01N63/10C07K1/14C07K1/18C07K1/34C07K1/36C12P21/00
Inventor 饶胜其张秋艳杨振泉胡源尹永祺方维明
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap