Biological mildewproof agent and preparation method thereof

An antifungal and biological technology, applied in the field of antibacterial, can solve the problems of expensive equipment, cumbersome operation, drug residues and other problems of physical control technology, and achieve the effects of fast reproduction, easy operation and high feasibility

Active Publication Date: 2018-04-06
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two methods have great disadvantages. The equipment of physical prevention technology is expensive and the operation is cumbersome, and people are also worried that radiation will be harmful to the human body; problems such as drug residues caused by chemical antiseptic technology are becoming more and more worrying.

Method used

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  • Biological mildewproof agent and preparation method thereof
  • Biological mildewproof agent and preparation method thereof
  • Biological mildewproof agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation method of a kind of biological antifungal agent of the present invention comprises the following steps:

[0041] (1) Preparation of B. amyloliquefaciens F1 fermentation broth

[0042]The B. amyloliquefaciens F1 strain was inoculated into a test tube containing 5 mL of nutrient broth at 2% inoculum, and the B. amyloliquefaciens F1 seed liquid was obtained by shaking at 30°C. The B. amyloliquefaciens F1 seed liquid was sucked and added to a conical flask with a loading of 40% fermentation medium at 2% inoculum, and cultured at 30° C. for 48 hours on a shaker to obtain a B. amyloliquefaciens F1 fermentation broth.

[0043] (2) Preparation of crude B. amyloliquefaciens F1 antimicrobial protein

[0044] First, take 1L of the above-mentioned B. amyloliquefaciens F1 fermentation broth, and carry out ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 15kDa to obtain an ultrafiltration concentrate. Then, ammonium sulfate was slo...

Embodiment 2

[0049] The preparation method of a kind of biological antifungal agent of the present invention comprises the following steps:

[0050] (1) Preparation of B. amyloliquefaciens F1 fermentation broth

[0051] The B. amyloliquefaciens F1 strain was inoculated into a test tube containing 5 mL of nutrient broth at 2% inoculum, and the B. amyloliquefaciens F1 seed liquid was obtained by shaking at 30°C. Aspirate B. amyloliquefaciens F1 seed liquid, add 2% inoculum to an Erlenmeyer flask with 20% fermentation medium, and culture at 32°C for 72 hours on a shaker to obtain B. amyloliquefaciens F1 fermentation broth.

[0052] (2) Preparation of crude B. amyloliquefaciens F1 antimicrobial protein

[0053] First, take 1L of the above-mentioned B. amyloliquefaciens F1 fermentation broth, and carry out ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 15kDa to obtain an ultrafiltration concentrate. Then, ammonium sulfate was slowly added to the ultrafiltr...

Embodiment 3

[0060] The preparation method of a kind of biological antifungal agent of the present invention comprises the following steps:

[0061] (1) Preparation of B. amyloliquefaciens F1 fermentation broth

[0062] The B. amyloliquefaciens F1 strain was inoculated into a test tube containing 5 mL of nutrient broth at 2% inoculum, and the B. amyloliquefaciens F1 seed liquid was obtained by shaking at 30°C. The B. amyloliquefaciens F1 seed liquid was sucked, added to a conical flask with a loading of 30% fermentation medium at 2% inoculum, and cultured at 37° C. for 70 hours on a shaker to obtain a B. amyloliquefaciens F1 fermentation broth.

[0063] (2) Preparation of crude B. amyloliquefaciens F1 antimicrobial protein

[0064] First, take 1 L of the above-mentioned B. amyloliquefaciens F1 fermentation broth, and carry out ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 8 kDa to obtain an ultrafiltration concentrate. Then, ammonium sulfate was slow...

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PUM

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Abstract

The invention discloses a biological mildewproof agent and a preparation method thereof. The biological mildewproof agent comprises the following raw materials in terms of 100 parts of water by weight: 2 to 8 parts of antibacterial protein or a crude antibacterial protein product, 0.5 to 3 parts of a film forming agent, 0.5 to 5 parts of a plasticizer and 0.5 to 4 parts of a cross-linking agent. The preparation method comprises the following steps: adding water into the film forming agent, completely dissolving the film forming agent, then adding the antibacterial protein, the plasticizer andthe cross-linking agent, and carrying out uniform mixing so as to obtain a biological mildewproof solution. According to the invention, the crude antibacterial protein product is a fermentation brothof B.amyloliquefaciens F1 and is obtained by ultrafiltration and ammonium sulfate fractional precipitation, while the antibacterial protein is prepared from the above-mentioned crude antibacterial protein product through further separation means like dialysis, ion exchange and gel chromatography. The biological mildewproof solution obtained by adding a main antibacterial substance namely the antibacterial protein inhibits the growth of Aspergillus flavus, has characteristics like safety and high efficiency, and solves the problems of high price and complex operation for physical preservation and a problem of pesticide residue brought by chemical preservation. The biological mildewproof agent provided by the invention has the advantages of economy, high efficiency, convenient use, and greatapplication prospects in the fields of agriculture and food preservation.

Description

technical field [0001] The invention relates to the technical field of antibacterial, in particular to a preparation method of a biological antifungal agent. Background technique [0002] Aspergillus flavus is a deficient fungus, the colony grows rapidly, the surface is yellow-green, and the back is slightly reddish-brown. Bacterial growth usually appears many branched hyphae, forming mycelium. Degradative enzymes or proteins are secreted to break down complex nutrients once the mycelium is formed. Monobranched hyphae are usually not visible to the naked eye, but the thick mats of hyphae produced by conidia can be easily seen. Aflatoxins, the secondary metabolites produced by Aspergillus flavus, belong to a large category of mycotoxins and are widely found in various cereals (corn, rice), oil and oil crops (peanut oil, peanuts, nuts), fruits and vegetables, milk and In meat, it has teratogenic and carcinogenic hazards. [0003] There are physical methods, chemical method...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N37/46A01P1/00A01P3/00C12P21/00C07K1/36C07K1/34C07K1/18C07K1/14C12R1/07
CPCA01N37/46A01N63/10C07K1/14C07K1/18C07K1/34C07K1/36C12P21/00
Inventor 饶胜其张秋艳杨振泉胡源尹永祺方维明
Owner YANGZHOU UNIV
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