Silk fibroin-PLGA composite material having ordered micro-groove structures, and preparation method and application thereof
A technology of silk fibroin and composite materials, which is applied in the field of biomedical polymer materials, can solve the problems of poor cell responsiveness and insufficient mechanical properties, and achieves simple and easy methods, high degradation behavior, and good biocompatibility Effect
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Embodiment 1
[0031] Weigh 10 g of anhydrous sodium carbonate, dissolve it in 2 L of ultrapure water, and prepare 5 g / L of Na 2 CO 3 aqueous solution. Put 20 g silkworm raw silk in it, heat and boil for 30 min. Take out the silk, wash it with a large amount of ultrapure water, and tear the silk sufficiently to wash away the sericin therebetween. Replace with new sodium carbonate solution, repeat the boiling and cleaning process. The degummed silk was placed in a blast drying oven at 60°C overnight.
[0032] Prepare a calcium chloride / ultrapure water / ethanol ternary solvent with a molar ratio of 1:8:2, and first mix CaCl 2 Add it into ultrapure water, heat to 80 °C, and add ethanol after it is completely dissolved. The dried silk was put into the ternary solvent in blocks according to a certain proportion, the ratio of silk to solvent was 10% (w / v), dissolved at 80 °C and stirred for 40 min.
[0033] After the silk fibroin solution was cooled to room temperature, it was poured into a d...
Embodiment 2
[0036] Weigh 10 g of anhydrous sodium carbonate, dissolve it in 2 L of ultrapure water, and prepare 5 g / L of Na 2 CO 3aqueous solution. Put 20 g silkworm raw silk in it, heat and boil for 30 min. Take out the silk, wash it with a large amount of ultrapure water, and tear the silk sufficiently to wash away the sericin therebetween. Replace with new sodium carbonate solution, repeat the boiling and cleaning process. The degummed silk was placed in a blast drying oven at 60°C overnight.
[0037] Prepare a calcium chloride / ultrapure water / ethanol ternary solvent with a molar ratio of 1:8:2, and first mix CaCl 2 Add it into ultrapure water, heat to 80 °C, and add ethanol after it is completely dissolved. The dried silk was put into the ternary solvent in blocks according to a certain proportion, the ratio of silk to solvent was 10% (w / v), dissolved at 80 °C and stirred for 40 min.
[0038] After the silk fibroin solution was cooled to room temperature, it was poured into a di...
Embodiment 3
[0041] Weigh 10 g of anhydrous sodium carbonate, dissolve it in 2 L of ultrapure water, and prepare 5 g / L of Na 2 CO 3 aqueous solution. Put 20 g silkworm raw silk in it, heat and boil for 30 min. Take out the silk, wash it with a large amount of ultrapure water, and tear the silk sufficiently to wash away the sericin therebetween. Replace with new sodium carbonate solution, repeat the boiling and cleaning process. The degummed silk was placed in a blast drying oven at 60°C overnight.
[0042] Prepare a calcium chloride / ultrapure water / ethanol ternary solvent with a molar ratio of 1:8:2, and first mix CaCl 2 Add it into ultrapure water, heat to 80 °C, and add ethanol after it is completely dissolved. The dried silk was put into the ternary solvent in blocks according to a certain proportion, the ratio of silk to solvent was 10% (w / v), dissolved at 80 °C and stirred for 40 min.
[0043] After the silk fibroin solution was cooled to room temperature, it was poured into a d...
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