Actinomucor elegans strain used for producing lipase from vegetable oil and fat deodorizing distillates

A technology of Mucor radiatae and deodorized distillate is applied in the field of biological fermentation engineering, which can solve the problems of difficulty in separating sterols and vitamin E, environmental pollution, and high production costs, and achieves good promotion and application significance, significant economic benefits, and production technology. easy effect

Active Publication Date: 2018-04-10
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But also there are some weak points in prior art, main reason is: one, sterol, vitamin E and free fatty acid, glyceride combine together, and all are fat-soluble compound, the physicochemical property difference between them is little, Therefore, it is very difficult to separate sterols and vitamin E from them, which undoubtedly increases the difficulty and cost of extraction; secondly, as a by-product, the content of sterols and vitamin E in the deodorized distillate is actually very little, and the content of different vegetable oils The composition of deodorized distillates is different, and the content of vitamin E and sterols is also different. For example, in the deodorized distillates of rice bran oil and cottonseed oil, the average content of...

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  • Actinomucor elegans strain used for producing lipase from vegetable oil and fat deodorizing distillates
  • Actinomucor elegans strain used for producing lipase from vegetable oil and fat deodorizing distillates

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Embodiment 1

[0026] This embodiment is just Mucor actinosa ( Actinomucor elegans , accession number: GDMCC No. 60214) is briefly introduced as follows.

[0027] (1) Strain enrichment:

[0028] The screening source samples collected near Grain and Oil Co., Ltd. (or oil factory) were added to the high-temperature sterilized enrichment medium, and cultured in a constant temperature incubator at 25 °C for 96 h to obtain the enriched mixed bacterial strain.

[0029] The composition of the enrichment medium is as follows ( / mL): 20 g of cottonseed oil deodorized distillate, 5 g of yeast extract, 5 g of sodium chloride, 2 g of potassium dihydrogen phosphate, and 18 g of agar.

[0030] (2) Selective cultivation

[0031] Dilute the mixed bacteria in step (1) in a serial gradient, inoculate them on a selective plate medium (selective medium) with vegetable oil deodorized distillate as the only carbon source, and cultivate them in a constant temperature incubator at 25°C After 96 h, the microbial ...

Embodiment 2

[0044] Utilize Mucor actinosa gc-1 obtained from screening in Example 1 ( Actinomucor elegans ) strains are cultured in the enzyme-producing medium, and their production performance is specifically determined;

[0045] The enzyme production medium includes the following components ( / mL): 50 g of vegetable oil deodorized distillate, and 2 g of yeast extract. The culture conditions are as follows: culture in a shaker for 120 h at 28°C and 150 rpm.

[0046] The enzyme activity of lipase is determined by titration method. The technical principle is: lipase belongs to carboxyl ester hydrolase, and within a certain temperature and pH range, it can gradually hydrolyze triglyceride into glycerol and fatty acid, and the released The fatty acid can be neutralized and titrated with standard alkali solution, the reaction end point is indicated by a pH meter or phenol peptide indicator solution, and the enzyme activity is calculated according to the amount of iodine consumed (the reactio...

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Abstract

The invention belongs to the technical field of biological fermentation engineering, and more specifically relates to actinomucor elegans strain used for producing lipase from vegetable oil and fat deodorizing distillates. The actinomucor elegans strain belongs to gc-1 actinomucor elegans, and the preservation number is GDMCC No.60214. The bacteria strain is capable of taking vegetable oil and fatdeodorizing distillates as a single carbon source, can be used for fermentation production of lipase taking the vegetable oil and fat deodorizing distillates as raw materials; the highest enzyme activity is 10.89U/ml, and the application prospect is promising. The related raw materials are cheap; the preparation technology is simple and convenient; no environment pollution is caused; the economical benefits are excellent; and the economical value and the popularization application meaning are excellent.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and specifically relates to a patent application for a mucormyces radiata strain that uses vegetable oil deodorized distillates to produce lipase. Background technique [0002] Deodorization distillate is a by-product collected during steam distillation of vegetable oil deodorization process or physical refining process. This substance has the appearance and consistency similar to acid residue, so it is often attributed to scum oil, mainly tocopherol, A complex fatty acid mixture of sterols, sterol esters, fatty acid glycerides, carbohydrates and other impurities. The content of each component varies greatly depending on the type of vegetable oil and the production process. At present, many domestic vegetable oil deodorization distillates are treated as waste by manufacturers, which not only causes a huge waste of raw materials, but also causes environmental pollution....

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/20C12R1/785
CPCC12N9/20C12Y301/01003C12N1/145C12R2001/785
Inventor 张海燕王刚边涛
Owner HENAN UNIVERSITY
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