Silica fluorescent immunolabeled probe based on fluorescence resonance energy transfer as well as preparation method and application of silica fluorescent immunolabelled probe

A fluorescence resonance energy and silicon dioxide technology, which is applied in the field of biological analysis and immune analysis, can solve the problems of slow carrier metabolism, toxic and side effects of organisms, etc.
CN107976538APending Publication Date: 2018-05-01ANHUI NORMAL UNIV
6 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ANHUI NORMAL UNIV
Publication Date
2018-05-01

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a silica fluorescent immunolabeled probe based on fluorescence resonance energy transfer as well as a preparation method and application of the silica fluorescent immunolabelledprobe. The preparation method comprises the steps of using mesoporous silica nanoparticles as matrix, and constructing a fluorescence resonance energy transfer system (RB-BODIPY-FRET) by doping rhodamine B and BODIPY. The fluorescent labeling probe is coupled by using a sodium periodate oxidation antibody, so that a reliable basis is provided for the super-sensitive detection and immunoassay of nano-drug carriers. Compared with the prior art, the silica fluorescent immunolabeling probe is prepared by means of codoping of fluorescent dyes, i.e., the BODIPY and the rhodamine B, and fluorescenceresonance energy transfer occurs between the two fluorescent dyes, so that the fluorescence intensity is increased, and quenching is reduced; the fluorescent labeled probe is coupled by using the sodium periodate oxidation antibody, so that the coupling efficiency is improved, and the influence of a cross-linking agent on the activity of the antibody is eliminated.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the fields of biological analysis and immunoanalysis, and in particular relates to a silica fluorescent immunolabeling probe based on fluorescence resonance energy transfer, a preparation method and an application. Background technique

[0002] Polysuccinimide (PSI), N-(3-aminopropyl) imidazole (NAPI) and oleylamine (OAm) react together to obtain PSI-OAm-NAPI amphiphilic polymeric nanoparticles (MFAP), which is a kind of polymer Nano drug carrier. Polymer emulsion delivery systems have been widely used to encapsulate drugs, and many polymer nano-drug delivery systems have been approved in clinical applications or are being used in clinical trials for the treatment of cancer. The metabolism after entering the organism is inseparable from the health of the organism. Excessive drug dosage may cause slow metabolism of the carrier and cause toxic side effects on the organism. Therefore, the quantitative determination of the drug c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More