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Method for performing mixed fermentation on lactobacillus acidophilus and clostridium butyricum as well as fermentation equipment thereof

A technology of Lactobacillus acidophilus and Clostridium butyricum, applied in the biological field, can solve the problems that Clostridium butyricum is easy to contaminate miscellaneous bacteria, reduce the utilization value of raw materials, and the production level is not high, so as to release the growth and reproduction ability of live bacteria, inhibit The growth of miscellaneous bacteria and the effect of strong practicality

Inactive Publication Date: 2018-05-08
湖北华扬科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Lactobacillus acidophilus is generally obtained through liquid fermentation, which has strict requirements on equipment and control, and is easy to die during high-temperature drying. In production, the freeze-drying process is generally used, and the cost is high
Clostridium butyricum can be obtained through liquid fermentation or solid state fermentation, but the current domestic production level is generally not high and the cost is relatively high
The production of Clostridium butyricum by solid-state fermentation is easy to contaminate miscellaneous bacteria and reduce the utilization value of raw materials

Method used

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  • Method for performing mixed fermentation on lactobacillus acidophilus and clostridium butyricum as well as fermentation equipment thereof
  • Method for performing mixed fermentation on lactobacillus acidophilus and clostridium butyricum as well as fermentation equipment thereof
  • Method for performing mixed fermentation on lactobacillus acidophilus and clostridium butyricum as well as fermentation equipment thereof

Examples

Experimental program
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Effect test

no. 1 example

[0038] Please refer to the attached Figure 1~2 , the present invention provides a fermentation device 10, which includes a container body 100, and the container body 100 is provided with a one-way gas outlet channel.

[0039] Further, in this embodiment, the container body 100 is a cylindrical structure, which can be made of stainless steel, glass or porcelain. The diameter of the container body 100 can be 300-1000 mm, the height is 300-1000 mm, and the bottom and circumference are sealed.

[0040] The main body of the container 100 is used to accommodate the fermentation raw materials for the fermentation process. The one-way air outlet channel is provided on the main body of the container 100 to ventilate and discharge the hydrogen generated during the fermentation of Clostridium butyricum during the fermentation process, and at the same time relieve the effect of hydrogen on butyric acid. Metabolic inhibition produced by Clostridia. Clostridium butyricum is a strict anaer...

no. 2 example

[0046] This embodiment provides a mixed fermentation method of Lactobacillus acidophilus and Clostridium butyricum.

[0047] Mix 3 parts of yeast extract, 2 parts of brown sugar, 1 part of soybean meal powder, and 94 parts of water, adjust the initial pH to 7.5, sterilize at 121°C for 30 minutes, and cool to 37°C to obtain Lactobacillus acidophilus liquid medium. Take a Lactobacillus acidophilus strain preserved in a glycerol tube, and connect the strain to the Lactobacillus acidophilus culture medium at an inoculation amount of 0.1%, and then amplify and expand the culture step by step, which is the Lactobacillus acidophilus bacterial liquid. The number of live bacteria of Lactobacillus acidophilus is 220 million CFU / g.

[0048] Mix 2 parts of glucose, 1.5 parts of peptone, 1.5 parts of yeast extract powder, 0.1 part of ammonium sulfate, 0.1 part of sodium carbonate, 0.3 part of corn steep liquor, 0.05 part of manganese sulfate, 0.05 part of magnesium sulfate, and 95 parts of...

no. 3 example

[0054] The principle of the mixed fermentation method of Lactobacillus acidophilus and Clostridium butyricum provided in this embodiment is the same as that provided in the second embodiment, the difference is that the parameters are different, and the difference is as follows:

[0055] Mix 2 parts of yeast extract, 1 part of brown sugar, 0.5 part of soybean meal powder, and 92 parts of water, adjust the initial pH to 7, sterilize at 120°C for 35 minutes, and cool to 36°C to obtain Lactobacillus acidophilus liquid medium. Take the Lactobacillus acidophilus bacterial strain preserved in a glycerol tube, transfer the bacterial strain to the Lactobacillus acidophilus culture medium according to the inoculum amount of 0.08%, and enlarge and expand it step by step to obtain the Lactobacillus acidophilus bacterial liquid.

[0056] Mix 1 part of glucose, 1 part of peptone, 1 part of yeast extract powder, 0.05 part of ammonium sulfate, 0.05 part of sodium carbonate, 0.2 part of corn st...

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Abstract

The invention relates to the field of biology and provides a method for performing mixed fermentation on lactobacillus acidophilus and clostridium butyricum. The method comprises the following steps:inoculating the lactobacillus acidophilus on an unsterilized solid culture medium to perform fermentation, and then inoculating clostridium butyricum liquid to perform fermentation. The synergistic inhibition effect on infectious microbes by the lactobacillus acidophilus and the clostridium butyricum can be utilized, the growth of the infectious microbes can be effectively inhibited and the infectious microbes in the production process can be effectively controlled; furthermore, under the condition of not sterilizing the raw materials, the lactobacillus acidophilus and the clostridium butyricum are fermented, so that the cost is reduced and the practicability is high. In addition, the invention also provides fermentation equipment. The fermentation equipment can be applied to solid fermentation production of anaerobic bacteria or facultative anaerobic bacteria such as the clostridium butyricum and the lactobacillus acidophilus; and the fermentation equipment discharges volatile metabolic products such as hydrogen and carbon dioxide to the external air through a gas outlet pipe, relieves metabolic inhibition, and can release viable bacteria to improve the growth and reproduction capacity and increase the number of the fermented viable bacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a mixed fermentation method of Lactobacillus acidophilus and Clostridium butyricum and equipment thereof. Background technique [0002] Lactobacillus acidophilus is generally obtained through liquid fermentation, which requires strict equipment and control, and is easy to die during high-temperature drying. In production, freeze-drying is generally used, which is expensive. Clostridium butyricum can be obtained by either liquid fermentation or solid state fermentation, but the current domestic production level is generally not high and the cost is relatively high. The production of Clostridium butyricum by solid-state fermentation is likely to contaminate miscellaneous bacteria and reduce the utilization value of raw materials. Contents of the invention [0003] The object of the present invention is to provide a production method of mixed fermentation of Lactobacillus acidophilus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12M1/00C12R1/145C12R1/23
CPCC12M23/38C12M29/20C12N1/20
Inventor 张东晓詹志春吴学军周樱凌华云刘文悦张成杰
Owner 湖北华扬科技发展有限公司
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