Method for removing antibiotic resistance genes through ionizing irradiation

A technology of antibiotic resistance and ionizing radiation, applied in the field of waste treatment, can solve the problems of land occupation, high moisture content of antibiotic residues, and low calorific value

Inactive Publication Date: 2018-05-18
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotic residues have high moisture content and low calorific value, and additional fuel is required during the incineration process, resulting in high processing costs
Landfilling the fungal residue takes up a lot of land, and the lea

Method used

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  • Method for removing antibiotic resistance genes through ionizing irradiation
  • Method for removing antibiotic resistance genes through ionizing irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Get the solid-liquid mixture of erythromycin slag (the water content is 93%, the total suspended solids content is 72g / L, wherein the proportion of organic solids is 80%) to detect the concentration of erythromycin resistance gene and the active substance erythromycin The amount of prime A. Then put the solid-liquid mixture of the bacteria residue into the irradiation container, and use Co 60 Gamma rays (radiation activity 3.6×10 14 Bq), irradiated near the central channel, the dose rate is about 240Gy / min. The absorbed dose was controlled to be 5kGy and 10kGy by adjusting the irradiation time. After the irradiation, the concentration of erythromycin resistance gene and the concentration of residual active substance erythromycin A in the bacteria residue were detected under different absorbed doses.

[0027] The residual erythromycin in the bacterial residue was detected by high performance liquid chromatography. First use organic solvent to extract it from the bact...

Embodiment 2

[0033] Get the erythromycin thiocyanate bacterium residue solid-liquid mixture (water content is 92%, total suspended solids content is 75g / L, wherein the proportion of organic solids is 77%) to detect the concentration and activity of erythromycin resistance gene The amount of substance erythromycin A. It is then placed in an irradiation container with Co 60 Gamma rays (radiation activity 3.6×10 14 Bq), irradiated near the central channel, the dose rate is about 240Gy / min. The absorbed radiation dose was controlled to be 10kGy, 20kGy and 30kGy by adjusting the irradiation time. After the irradiation, the concentration of erythromycin A remaining in the residue of erythromycin thiocyanate and the amount of resistance gene were detected under different absorbed doses.

[0034] The residual erythromycin A in the bacteria residue was first extracted with organic solvent, and then detected by liquid chromatography. The specific detection method is the same as in Example 1.

...

Embodiment 3

[0038] Take penicillin slag solid-liquid mixture (water content is 88%, total suspended solids content is 116g / L, wherein the proportion of organic solid is 87%) to detect the concentration of penicillin resistance gene and the amount of penicillin. Then put the solid-liquid mixture of the bacteria residue into the irradiation container, and use Co 60 Gamma rays (radiation activity 3.6×10 14 Bq), irradiated near the central channel, the dose rate is about 240Gy / min. The absorbed radiation dose was controlled to be 5kGy and 10kGy by adjusting the irradiation time. After the irradiation, the residual penicillin concentration in the penicillin residue under different absorbed doses was detected and the qualitative detection of the resistance gene was carried out.

[0039] For the residual penicillin in the bacteria residue, it was first extracted with an organic solvent, and then detected by liquid chromatography. Wherein, the liquid chromatograph used is a high-performance li...

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Abstract

The invention discloses a method for removing antibiotic resistance genes through ionizing irradiation. Antibiotic mushroom dregs are treated with an ionizing irradiation technology (including gamma-rays and high-power electron beams generated by an electronic accelerator) to damage DNA of microbial cells, so that the resistance genes are effectively removed, and residual antibiotics can be decomposed simultaneously. The method is efficient and wide in application range; radiation can be performed at the normal temperature, no chemical reagents or only a few chemical reagents are required to be added, and no secondary pollution can be caused. The method can be applied to removal of the resistance genes in the antibiotic mushroom dregs, can be applied to removal of the resistance genes in water, soil and sludge and has wide application prospects in the environmental field.

Description

technical field [0001] This article belongs to the field of waste treatment, in particular, this article relates to a method for removing the resistance gene in the hazardous solid waste of the pharmaceutical industry-antibiotic bacteria residue. Background technique [0002] my country is the world's largest producer and user of antibiotics. According to statistics, in 2013, the output of antibiotics in my country was 248,000 tons, and the consumption was 162,000 tons. A large amount of solid waste-antibiotic residue is produced in the fermentation production process of antibiotic drugs. Based on the calculation that 1 ton of antibiotics produces 8 to 10 tons of wet fungal residue, the annual output of fungal residue is as high as 2 million tons or more. The main components of the fungus residue are bacteria, unused medium, metabolites of the fermentation process, and residual antibiotics and antibiotic resistance genes (Antibiotic Resistance Genes). Although the mushroo...

Claims

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Application Information

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IPC IPC(8): C02F1/30C02F11/00B09B3/00C05F11/00C10L5/48A23K20/195C02F101/30
CPCA23K20/195C10L5/48C02F1/305C02F11/00C05F11/00B09B3/00C02F2101/30B09B3/0075Y02E50/30C02F1/307C02F2103/343B09B5/00C10L9/00C10L2290/36C10L2270/04C05F17/10C05F17/30Y02W30/40Y02P20/145A23K10/00C02F11/004C05F5/008B01J19/081
Inventor 王建龙初里冰
Owner TSINGHUA UNIV
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