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Sargassum henslouianum ethyl acetate extract separating component E, preparation method and application thereof in marine fouling organism control

A technology of ethyl acetate extraction and Sargassum Heinzii, which is applied in botany equipment and methods, chemicals for biological control, applications, etc., to achieve the effects of large promotion potential, high safety, and wide sources

Active Publication Date: 2018-05-22
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on whether Sargassum henzii contains active ingredients against larvae attachment and spore germination of marine fouling organisms and whether it can be applied to the field of marine fouling biocontrol

Method used

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  • Sargassum henslouianum ethyl acetate extract separating component E, preparation method and application thereof in marine fouling organism control
  • Sargassum henslouianum ethyl acetate extract separating component E, preparation method and application thereof in marine fouling organism control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Sargassum Heinzki ethyl acetate extract separation component E is extracted from Sargassum heinzii, and its separation and extraction steps include:

[0023] (1) Sargassum henslowianum C.Agardh was extracted three times with a 95% ethanol aqueous solution by volume fraction, and the extracts were combined to obtain an ethanol extract, concentrated by vacuum rotary evaporation at 45° C., and ethanol was removed to obtain a paste extract;

[0024] (2) The paste extract is resuspended with water at a ratio of 6 ml per gram, first extracted 3 times with an equal volume of petroleum ether, discarding the petroleum ether extraction components, and extracting the remaining water layer 3 times with an equal volume of ethyl acetate , the combined ethyl acetate extract was concentrated and dried to obtain the ethyl acetate extract;

[0025] (3) Carry out chromatographic separation of the ethyl acetate extract in an atmospheric silica gel column (silica gel 8000g, column length 18...

Embodiment 2

[0028] Experimental group: Dissolve component E of the ethyl acetate extract of Sargassum henzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of fraction E of the ethyl acetate extract of Sargassum Heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then 13ml of seawater was added.

[0029] Control group: add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), and distribute it evenly on the bottom of the petri dish, and then add 13ml of seawater after volatilization is complete.

[0030] Blank group: add 13ml of seawater into ...

Embodiment 3

[0036] Experimental group: Dissolve component E of the ethyl acetate extract of Sargassum henzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of Fraction E isolated from the ethyl acetate extract of Sargassum Heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then 13ml of seawater was added.

[0037] Control group: Add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), so that the solvent is evenly distributed on the bottom of the petri dish, and after the solvent is completely evaporated, add 13ml of seawater.

[0038] Blank ...

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Abstract

The invention discloses a sargassum henslouianum ethyl acetate extract separating component E, a preparation method and application thereof in marine fouling organism control. When low dosage of sargassum henslouianum ethyl acetate extract separating component E is covered on a solid surface, the sargassum henslouianum ethyl acetate extract separating component E has a remarkable effect on inhibiting barnacle and mussel larvae settlement so as to be used for preparing a marine fouling organism control agent. The sargassum henslouianum ethyl acetate extract separating component E is extracted from sargassum henslouianum, is a natural organic compound, cannot pollute a water environment, cannot be enriched in organism caused by transferring through a food chain, is environmentally-friendly and high in safety, can effectively inhibit marine organism settlement, does not contain heavy metal elements such as copper and tin at the same time, has a favorable social benefit from the perspective of environmental protection, is wide in sources, simple and convenient to separate and prepare, suitable for mass production, and large in popularization potential, and has a favorable application prospect in marine fouling organism control.

Description

Technical field: [0001] The invention belongs to the field of natural products, and in particular relates to the separation component E of ethyl acetate extract of Sargassum henzii and its preparation method and application in marine fouling biological control. Background technique: [0002] Marine fouling organisms refer to all kinds of organisms that fix or inhabit underwater parts of ships and artificial facilities and have adverse effects on human economic activities. The metal corrosion process causes corrosion and perforation; increases the dynamic load effect, causing man-made facilities to drift, unbalance or even overturn; competes with breeding objects for attachment substrates and bait, hinders their growth and development, and reduces the quality of aquatic products. Therefore, in the process of developing the marine economic industry, marine fouling biological control is always an indispensable and important part. [0003] Marine fouling organisms are composed ...

Claims

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Application Information

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IPC IPC(8): A01N65/03A01P17/00
CPCA01N65/03
Inventor 曹文浩吴慧严涛卢虹玉林明晴章超桦韩帅帅
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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