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Application of polymalic acid chelated salts as trace element supplement

A polymalic acid and trace element technology, applied in application, microorganism-based methods, microorganisms, etc., can solve the problems of high gastrointestinal irritation, slow growth, low bioavailability, etc., and achieve improved bioavailability and accelerated absorption and utilization. , the effect of high bioavailability

Inactive Publication Date: 2018-05-22
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In agriculture, lack of iron, zinc and other elements, plants are prone to leaf yellowing, slow growth and other problems
[0004] Iron or zinc supplements currently on the market are mainly in the form of inorganic salts (ferric sulfate, zinc sulfate, etc.) Poor resistance, gastrointestinal irritation and other problems; iron supplements used in agriculture, mainly in the form of ferrous sulfate, EDTA-chelated iron, etc., have problems such as easy oxidation, poor stability, and damage to soil quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the preparation method of polymalate chelated iron

[0025] The preparation method of polymalate chelated iron, concrete method is as follows:

[0026] (1) Strain seed culture: inoculate a single colony of Aureobasidium pullulans (Aureobasidium pullulans) cultured by streaking in a 500 mL shaker flask with a liquid content of 50 mL, and cultivate for 200 rpm at a culture temperature of 25 ° C. After 72 hours, the seed culture solution was obtained; the components of the medium during the cultivation process were as follows: glucose 70g / L, ammonium nitrate 4g / L, KH 2 PO 4 0.01g / L, ZnSO 4 0.1g / L, MgSO 4 0.1g / L, corn steep liquor 0.05g / L and CaCO 3 15g / L;

[0027] (2) Calcium polymalate fermentation process: Inoculate the seed culture solution of step (1) into a 300L fermenter with a liquid capacity of 220L, and cultivate it at a culture temperature of 25°C, a rotation speed of 200-400rpm, and an aeration ratio of 1:1.2. 156 hours, the fermentation p...

Embodiment 2

[0029] Embodiment 2, the application of polymalate chelated iron

[0030] Polymalate chelated iron was made into a water-soluble nutritional supplement at a concentration of 50 mg / L, and EDTA chelated iron was used as a contrast, and the economic crops of cabbage were sprayed; two months later, detection was carried out, and the free iron content in the leaves was compared with EDTA- Iron has increased by 1.4 times, and the growth of Chinese cabbage is better than that of the control group.

Embodiment 3

[0031] Embodiment 3, the preparation method of polymalic acid chelated zinc

[0032] Prepare the calcium polymalate product according to the method of Example 1, then filter the polymalic acid fermentation broth with plate and frame filtration or membrane separation filtration (ultrafiltration membrane or inorganic ceramic membrane), remove the fermentation thalline, and obtain the calcium polymalate containing After the fermented clear liquid was concentrated, according to the volume of the fermented concentrated liquid, an ethanol solution with a volume fraction of 95% equivalent to 3 times the volume of the fermented concentrated liquid was added, and frozen at 4° C. for 24 hours to obtain a calcium polymalate precipitate. According to the calcium-zinc molar ratio of 1:1.5, polycalcium malate and zinc sulfate were mixed and dissolved, and under the protection of nitrogen, the initial pH was 5.0, the reaction temperature was 37°C, and the reaction time was 64 minutes to obtai...

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PUM

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Abstract

The invention relates to application of polymalic acid chelated salts as trace element supplements, and the trace element supplements can be used to production of plants and animals. By utilizing thecharacteristics of high water solubility, easy degradation and the likes of the polymalic acid, the polymalic acid chelated salts are prepared into the trace element supplements; compared with traditional supplements, water solubility, stability, bioavailability and the likes of the product have been greatly improved; after the product is sprayed on citrus yellow leaves, the leaves can quickly turn to green, after the product is sprayed on Chinese cabbages, the content of free iron in the leaves is improved, and after the product is fed to livestock and poultry animals, absorption of trace elements can be effectively promoted, therefore, the polymalic acid chelated salts have a good application prospect in the fields of agriculture, livestock and poultry breeding, etc.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to the application of polymalic acid chelate salt as a trace element supplement in agriculture and livestock breeding. Background technique [0002] Polymalic acid (PMA) is produced by fermentation of Aureobasidium pullulans, and is polymerized in vivo with L-malic acid as the only monomer. It is a completely biodegradable polymer material. As an aliphatic polyester, PMLA has the advantages of high biocompatibility, biodegradability, bioabsorbability, safety and non-toxicity. [0003] Trace elements such as iron, zinc, magnesium, etc., play an irreplaceable role in the human body, animals and plants. In the human body, iron is an important component of hemoglobin. Its existence enables hemoglobin to normally complete the delivery of oxygen to cells and the discharge of carbon dioxide; zinc deficiency can cause a variety of diseases, poor growth and development, short stature, and...

Claims

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Application Information

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IPC IPC(8): C05D9/02C05G3/00A23K20/20A23K50/40C12P7/62C12R1/645
CPCA23K20/30A23K50/40C05D9/02C05G3/00C12P7/625
Inventor 邹祥韩冷郭涛李天夫陈力
Owner SOUTHWEST UNIVERSITY
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