Application of polymalic acid chelated salts as trace element supplement

A polymalic acid and trace element technology, applied in application, microorganism-based methods, microorganisms, etc., can solve the problems of high gastrointestinal irritation, slow growth, low bioavailability, etc., and achieve improved bioavailability and accelerated absorption and utilization. , the effect of high bioavailability

Inactive Publication Date: 2018-05-22
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In agriculture, lack of iron, zinc and other elements, plants are prone to leaf yellowing, slow growth and other problems
[0004] Iron or zinc supplements currently on the market are mainly in the form of inorganic salts (ferric sulfate, zinc sulfate, et

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Example 1. Preparation method of polymalic acid chelated iron

[0025] The preparation method of polymalic acid chelated iron is as follows:

[0026] (1) Strain seed culture: A single colony of Aureobasidium pullulans streaked culture was inoculated into a 500 mL shake flask with a liquid volume of 50 mL, and the culture time was at 25°C and 200 rpm. After 72 hours, the seed culture solution was obtained; the components of the culture medium were as follows: glucose 70g / L, ammonium nitrate 4g / L, KH 2 PO 4 0.01g / L, ZnSO 4 0.1g / L, MgSO 4 0.1g / L, corn steep liquor 0.05g / L and CaCO 3 15g / L;

[0027] (2) Calcium polymalate fermentation process: inoculate the seed culture solution of step (1) in a 300L fermenter with a liquid volume of 220L, culture at a culture temperature of 25°C, a rotation speed of 200-400rpm, and aeration ratio of 1:1.2 156 hours, the fermentation process uses CaCO 3 Control the pH of the fermentation broth to around 6.0 to form a polymalate calcium salt pr...

Example Embodiment

[0029] Example 2. Application of polymalic acid chelated iron

[0030] The polymalic acid chelated iron was made into a water-soluble nutritional supplement at a concentration of 50mg / L. EDTA chelated iron was used as a control and sprayed on cabbage cash crops; two months later, the test showed that the free iron content in the leaves was compared with EDTA- Iron increased by 1.4 times, and the growth of cabbage was better than the control group.

Example Embodiment

[0031] Example 3 Preparation method of polymalic acid chelated zinc

[0032] The polymalic acid calcium salt product was prepared according to the method of Example 1, and then the polymalic acid fermentation broth was filtered by plate and frame filtration or membrane separation filtration (ultrafiltration membrane or inorganic ceramic membrane) to remove the fermented cells to obtain calcium polymalate After the fermentation liquid is concentrated, according to the volume of the fermentation liquid, a 95% ethanol solution with a volume fraction of 3 times the volume of the fermentation liquid is added and frozen at 4°C for 24 hours to obtain a calcium polymalate precipitate. According to the calcium-zinc molar ratio of 1:1.5, the calcium polymalate and zinc sulfate are mixed and dissolved, and under the protection of nitrogen, the initial pH is 5.0, the reaction temperature is 37°C, and the reaction time is 64 minutes to prepare zinc polymalate chelate. The zinc content in the ...

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PUM

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Abstract

The invention relates to application of polymalic acid chelated salts as trace element supplements, and the trace element supplements can be used to production of plants and animals. By utilizing thecharacteristics of high water solubility, easy degradation and the likes of the polymalic acid, the polymalic acid chelated salts are prepared into the trace element supplements; compared with traditional supplements, water solubility, stability, bioavailability and the likes of the product have been greatly improved; after the product is sprayed on citrus yellow leaves, the leaves can quickly turn to green, after the product is sprayed on Chinese cabbages, the content of free iron in the leaves is improved, and after the product is fed to livestock and poultry animals, absorption of trace elements can be effectively promoted, therefore, the polymalic acid chelated salts have a good application prospect in the fields of agriculture, livestock and poultry breeding, etc.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to the application of polymalic acid chelate salt as a trace element supplement in agriculture and livestock breeding. Background technique [0002] Polymalic acid (PMA) is produced by fermentation of Aureobasidium pullulans, and is polymerized in vivo with L-malic acid as the only monomer. It is a completely biodegradable polymer material. As an aliphatic polyester, PMLA has the advantages of high biocompatibility, biodegradability, bioabsorbability, safety and non-toxicity. [0003] Trace elements such as iron, zinc, magnesium, etc., play an irreplaceable role in the human body, animals and plants. In the human body, iron is an important component of hemoglobin. Its existence enables hemoglobin to normally complete the delivery of oxygen to cells and the discharge of carbon dioxide; zinc deficiency can cause a variety of diseases, poor growth and development, short stature, and...

Claims

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Application Information

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IPC IPC(8): C05D9/02C05G3/00A23K20/20A23K50/40C12P7/62C12R1/645
CPCA23K20/30A23K50/40C05D9/02C05G3/00C12P7/625
Inventor 邹祥韩冷郭涛李天夫陈力
Owner SOUTHWEST UNIVERSITY
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