Active polypeptide extracted from hippophae rhamnoides fruits

Abstract

The invention discloses active polypeptide extracted from hippophae rhamnoides fruits. The molecular weight of the active polypeptide is between 3-10KD. An extraction method of the active polypeptidecomprises the following steps of pretreating raw materials, defatting seed powder, performing enzymolysis for the first time, performing enzymolysis for the second time, and performing purification; and the extraction method comprises the specific steps of after hippophae rhamnoides fruit seeds are defatted, performing preliminary enzymolysis with alkali protease, then performing enzymolysis for the second time with pepsin under the condition of alternate spaced auxiliary enzymolysis of bifrequency ultrasonic waves and microwave radiation, and purifying enzymatic hydrolysate through an ultra filtration membrane so as to obtain the active polypeptide. The active polypeptide and the extraction method thereof disclosed by the invention have the beneficial effects that (S)-1-amino-2-hydroxymethyl indoline added in the enzymolysis reaction process can generate a dimerization reaction and a terpolymerization under strong acid environment, polymerization products can generate electrophilic effects with the pepsin, and the protein structure of the pepsin is prevented from changing, so that the activity of the pepsin is prevented from reducing, and an enzymolysis reaction is accelerated.

Description

technical field [0001] The invention relates to the field of preparation of active polypeptides, in particular to an active polypeptide extracted from sea buckthorn. technical background [0002] Seabuckthorn is a deciduous shrub, which is characterized by drought tolerance, wind and sand resistance, and can survive on saline-alkali land, so it is widely used in water and soil conservation. Seabuckthorn is grown in large quantities in northwest China for desert greening. The content of vitamin C in seabuckthorn fruit is high, known as the king of vitamin C. Seabuckthorn is a general name for plants and their fruits. Distributed in North China, Northwest, Southwest and other places in China, Seabuckthorn is a medicinal and edible plant. The roots, stems, leaves, flowers, and fruits of seabuckthorn, especially the fruit of seabuckthorn, are rich in nutrients and biologically active substances, and can be widely used in many fields of the national economy such as food, medici...

AI Technical Summary

Problems solved by technology

[0003] There are many methods for extracting seabuckthorn peptides. The existing technology, such as the Chinese invention patent with the authorized public number CN 103421873 B, discloses a preparation method of seabuckthorn seed polypeptide with antibacterial effect. In the inventive method, seabuckthorn seed dregs are first After soaking treatment, it is hydrolyzed step by step with tannase, phytase, acid protease, and papain successively, and the polypeptide hydrolyzate is left to stand overnight at low temperature, and then macromolecular impurities are removed by low-temperature high-speed centrifugation, and the centrifuged supernatant is concentrated and dried Finally, the seabuckthorn seed antibacterial polypeptide was obtained. The method for preparing the seabuckthorn seed antibacterial polypeptide in this invention is too cumbersome, and the preparation time is too long, which greatly increases the time cost, and the efficiency of the antibacterial polypeptide is low.