Active polypeptide extracted from hippophae rhamnoides fruits

A technology of active peptides and seabuckthorn, which is applied to the functions of food ingredients, food science, and protein-containing food ingredients, etc., can solve the problems of low efficiency of antibacterial peptides, increased time cost, and long preparation time, so as to improve scientific and technological content and save energy. Effect of reduction of cost, prevention activity

Inactive Publication Date: 2018-05-25
海盐县凌特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many methods for extracting seabuckthorn peptides. The existing technology, such as the Chinese invention patent with the authorized public number CN 103421873 B, discloses a preparation method of seabuckthorn seed polypeptide with antibacterial effect. In the inventive method, seabuckthorn seed dregs are first After soaking treatment, it is hydrolyzed step by step with tannase, phytase, acid protease, and papain successively, and the polypeptide hydrolyzate is left to sta...

Method used

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Examples

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Embodiment 1

[0017] An active polypeptide extracted from seabuckthorn. The molecular weight of the active polypeptide is between 3-10KD. The active polypeptide has strong anti-oxidation ability and can be used as an anti-oxidation active ingredient.

[0018] The method for extracting active polypeptide from seabuckthorn comprises the following steps:

[0019] 1) Select seabuckthorn seeds, crush them and dry them at 55°C for 24 hours, crush them with a pulverizer and sieve them with a mesh number of 200-mesh to obtain seabuckthorn seed powder; 2) Place seabuckthorn seed powder in supercritical CO 2 In the extraction kettle, when the extraction temperature is 48°C, the extraction pressure is 28MPa, CO 2 Extract for 2.5 hours under the condition of a flow rate of 25L / h. After the extraction, take out the meal from the kettle, crush, sieve, and dry to obtain the defatted seabuckthorn seed powder; Dissolve in distilled water, adjust the temperature to 36°C, pH to 8.4, add 3000U / g alkaline prot...

Embodiment 2

[0021] An active polypeptide extracted from seabuckthorn, the extraction method comprises the following steps:

[0022] 1) Raw material pretreatment: select clean and healthy seabuckthorn seeds that have been peeled off and have no deterioration, no diseases and insect pests, put them in an oven after crushing, dry at 60°C for 36 hours, crush them with a grinder and sieve them. The mesh size of the sieve is 300 meshes to obtain seabuckthorn seed powder; select healthy seabuckthorn seeds to prevent active substances from being polluted by moldy products, crush and sieve to remove impurities in the seeds;

[0023] 2) Degreasing of seed powder: put seabuckthorn seed powder in supercritical CO 2 In the extraction kettle, when the extraction temperature is 52°C, the extraction pressure is 32MPa, CO 2 Extract for 3.0 hours under the condition of a flow rate of 30L / h. After the extraction, the meal is taken out from the kettle, and after crushing, sieving, and drying, the defatted s...

Embodiment 3

[0028] An active polypeptide extracted from seabuckthorn. The molecular weight of the active polypeptide is between 3-10KD. The active polypeptide has strong anti-oxidation ability and can be used as an anti-oxidation active ingredient.

[0029] The method for extracting the active polypeptide from seabuckthorn includes: raw material pretreatment, seed powder degreasing, primary enzymatic hydrolysis, secondary enzymatic hydrolysis, and purification, specifically including the following steps:

[0030] Raw material pretreatment: select clean and healthy seabuckthorn seeds that have been peeled off without deterioration, pests and diseases, crushed and placed in an oven, dried at 58°C for 24 hours, crushed with a grinder and sieved with a mesh of 240 mesh , to obtain seabuckthorn seed powder; select healthy seabuckthorn seeds to prevent active substances from being polluted by moldy products, and crush and sieve to remove impurities in the seeds;

[0031] Defatting of seed meal:...

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Abstract

The invention discloses active polypeptide extracted from hippophae rhamnoides fruits. The molecular weight of the active polypeptide is between 3-10KD. An extraction method of the active polypeptidecomprises the following steps of pretreating raw materials, defatting seed powder, performing enzymolysis for the first time, performing enzymolysis for the second time, and performing purification; and the extraction method comprises the specific steps of after hippophae rhamnoides fruit seeds are defatted, performing preliminary enzymolysis with alkali protease, then performing enzymolysis for the second time with pepsin under the condition of alternate spaced auxiliary enzymolysis of bifrequency ultrasonic waves and microwave radiation, and purifying enzymatic hydrolysate through an ultra filtration membrane so as to obtain the active polypeptide. The active polypeptide and the extraction method thereof disclosed by the invention have the beneficial effects that (S)-1-amino-2-hydroxymethyl indoline added in the enzymolysis reaction process can generate a dimerization reaction and a terpolymerization under strong acid environment, polymerization products can generate electrophilic effects with the pepsin, and the protein structure of the pepsin is prevented from changing, so that the activity of the pepsin is prevented from reducing, and an enzymolysis reaction is accelerated.

Description

technical field [0001] The invention relates to the field of preparation of active polypeptides, in particular to an active polypeptide extracted from sea buckthorn. technical background [0002] Seabuckthorn is a deciduous shrub, which is characterized by drought tolerance, wind and sand resistance, and can survive on saline-alkali land, so it is widely used in water and soil conservation. Seabuckthorn is grown in large quantities in northwest China for desert greening. The content of vitamin C in seabuckthorn fruit is high, known as the king of vitamin C. Seabuckthorn is a general name for plants and their fruits. Distributed in North China, Northwest, Southwest and other places in China, Seabuckthorn is a medicinal and edible plant. The roots, stems, leaves, flowers, and fruits of seabuckthorn, especially the fruit of seabuckthorn, are rich in nutrients and biologically active substances, and can be widely used in many fields of the national economy such as food, medici...

Claims

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Application Information

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IPC IPC(8): A23L33/18
CPCA23L33/18A23V2002/00A23V2200/30A23V2250/55
Inventor 董珊珊
Owner 海盐县凌特生物科技有限公司
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