Beta-cypermethrin degrading bacterium and application thereof

A technology of cypermethrin degradation and cypermethrin, which is applied in the direction of bacteria, isolated microorganisms, microorganisms, etc., can solve the problems that Bacillus licheniformis can efficiently degrade β-cypermethrin, etc.

Inactive Publication Date: 2018-05-29
SICHUAN UNIV
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Problems solved by technology

At present, the reported pyrethroid pesticide degrading bacteria include Bacillus, Pseudomonas, Alcaligenes, Aspergillus niger, Acidomonas,

Method used

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  • Beta-cypermethrin degrading bacterium and application thereof

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Embodiment Construction

[0011] The following examples illustrate the present invention, but the present invention is not limited to the following examples.

[0012] 1. Strain screening method

[0013] (1) Luria-Bertani medium (LB) prepared tryptone 10g, yeast extract 5.0g, sodium chloride 10g, Tween-802g, deionized water 1000mL, pH 7.0, sterilized at 121°C for 15min.

[0014] (2) Bacterial samples were collected from tea gardens where β-cypermethrin was applied for a long time, and about 200 g of soil samples were randomly collected.

[0015] (3) Acclimatization 5.0 g of bacterial sample was weighed, placed in 30 mL of LB medium with a concentration of β-cypermethrin of 100 μg / mL, and cultured with shaking at 30° C. and 180 r / min for 120 h. Then, according to the inoculum amount of 5.0% (v / v), the culture solution was transferred into 30 mL of LB medium with a concentration of β-cypermethrin of 200 μg / mL, and cultured with shaking at 30° C. and 180 r / min for 120 h. Repeat this operation, and subcul...

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Abstract

The invention discloses a Beta-cypermethrin degrading bacterium. The bacterial strain is bacillus licheniformis B-1; the GenBank accession number of 16S rDNA is HQ009796. The strain is from soil of atea garden, and is obtained through Beta-cypermethrin concentration gradient domestication, preliminary screening, separation and purification and secondary screening. The bacterial colony edge of thestrain is reddish color; the center is white; the surface is dry and has drapes; the edge is irregular; the single thallus is in a rod shape and is gram positive; the spore is elliptical and is mesic; the sporocyst is slightly inflated; glucose, arabinose, xylose and mannite are positive; gelatin and starch can be hydrolyzed; phenylalanine dehydrogenase and yolk lecithin enzyme are negative; theanaerobic growth can be realized. Under the proper conditions, the degrading rate of the strain on the Beta-cypermethrin with the concentration being 16.5mu g/mL in a culture medium is 94.12 percent.

Description

technical field [0001] The invention provides a β-cypermethrin degrading bacterium and its application, belonging to the field of biotechnology. Background technique [0002] Pyrethroid pesticides are a class of synthetic toxic or low-toxic biomimetic insecticides, which will be one of the most widely used and difficult to replace pesticide types in the world in the next few decades. At present, it is widely used to control leaf-eating or fruit-eating pests of fruits and vegetables, tea leaves, grains, etc., and its application area has accounted for more than 30% of the total application area of ​​pesticides, and it is still on the rise. β-Cypermethrin is a commonly used type of pyrethroid pesticides. It is insoluble in water and its stability period can reach more than two years at room temperature. β-cypermethrin has strong toxicity to some non-target organisms such as fish, shrimp, crab and other aquatic organisms, and is also potentially harmful to mammals. Therefore, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A23L5/20B09C1/10C12R1/10
CPCA23L5/28B09C1/10C12N1/02C12N1/20C12N1/205C12R2001/10
Inventor 贾冬英蒋肇样姚开赵甲元迟原龙
Owner SICHUAN UNIV
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