Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methylotrophic bacillus and preparation method and application of inoculum containing the microorganism

A methylotrophic and microbial agent technology, applied in the biological field, can solve problems such as abnormal fruit expansion, food safety, and drug residues, and achieve the effects of avoiding pesticide residues and food safety, promoting growth and development, and accelerating synthesis and transformation

Active Publication Date: 2021-03-30
DALIAN UNIV OF TECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the strawberry wilt occurs, it will cause a decrease in the seed setting rate, abnormal fruit expansion, a decrease in quality, and a substantial decrease in yield, which will seriously affect the economic benefits and sustainable development of the strawberry industry.
In the past, chemical fungicides were used to control strawberry wilt, but the large-scale use of chemical fungicides is likely to cause problems such as environmental pollution, ecological imbalance, drug residues, and food safety. Therefore, low-cost, high-efficiency, environmentally friendly and drug-free Biological methods to prevent and control strawberry wilt have attracted much attention

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methylotrophic bacillus and preparation method and application of inoculum containing the microorganism
  • Methylotrophic bacillus and preparation method and application of inoculum containing the microorganism
  • Methylotrophic bacillus and preparation method and application of inoculum containing the microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation, screening and identification of methylotrophic Bacillus Z-1

[0031] The bacterial strain of the present invention is isolated from the soil of the strawberry planting area of ​​Dalian Jinzhou National Agricultural Science and Technology Park.

[0032] 1. Isolation and screening of methylotrophic Bacillus Z-1

[0033] (1) Weigh 10 g of the collected soil sample into a Erlenmeyer flask filled with 90 mL of PBS buffer solution, and add glass beads (to help the sample evenly disperse in PBS buffer solution), shake on a shaker at 30°C and 160 rpm 30min, leave standstill 10min, prepare soil bacteria suspension (i.e. 10 -1 Soil dilution); then carry out 10-fold serial dilution with PBS buffer solution to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 The soil suspension was diluted, and 100 μL of the soil bacterial suspension with different concentration gradients were taken respectively, spread evenly on the broth agar plate (NA), and cultivated...

Embodiment 2

[0046] Embodiment 2: the preparation of microbial bacterial agent

[0047] (1) Preparation of seed solution: transfer the methylotrophic Bacillus Z-1 strain stored on the NA slant to the NA plate, place it in a constant temperature incubator at 30°C for 24 hours, and pick it with a sterilized inoculation loop. Take a single colony and inoculate it into the seed liquid medium, and culture it on a shaking table at 30°C for 14 hours to obtain the seed liquid. The formula of the seed liquid culture medium is: peptone 1.6% (m / V), yeast powder 1.0% (m / V), sodium chloride 0.5% (m / V), pH value 7.0-7.2.

[0048] (2) Preparation of fermented liquid: Inoculate the cultured seed liquid into a large amount of fermentation medium at a ratio of 5.0% (volume ratio), culture at 30°C for 48 hours to obtain the fermented liquid, and count the bacteria on the plate for the cultured fermented liquid , the number of viable bacteria reached 1.0×10 9 CFU / mL or more for use. The formula of mass fer...

Embodiment 3

[0050] Embodiment 3: microbial bacterial agent is used for preventing and treating strawberry wilt

[0051] Select the Fengxiang strawberry variety as the experimental object. After the strawberry seeds start to germinate, they are sown into the seedling tray. 7 spores / mL spore solution of Fusarium wilt fungus for 20 minutes, and then transplanted into plastic pots filled with strawberry planting soil. Dilute the microbial agent with irrigation water to a viable count of 1ⅹ10 6 CFU / mL~1ⅹ10 7 CFU / mL, according to 1m 3 / mu, 3m 3 / mu and 5m 3 The amount per mu was irrigated in the soil near the root of the strawberry plant, and the irrigation with clear water was used as a blank control, and 3 repetitions were established for each treatment. The interval of irrigation was 15 days, and the biocontrol experiment lasted for 2 months, and the incidence rate and biological control effect were observed.

[0052] The incidence and control effects are shown in the table below:

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a methylotrophic Bacillus Z‑1 (Bacillus methylotrophicus Z‑1) with a preservation number of CGMCC NO.15004, and a microbial agent containing the methylotrophic Bacillus Z‑1, and also provides The application of the methylotrophic bacillus or microbial agent in the prevention and treatment of strawberry wilt is described. The methylotrophic bacillus and the microbial agent thereof provided by the invention have significant antagonism to the strawberry wilt, can prevent the occurrence of the strawberry wilt, and the control efficiency is over 80%. The use of the strain and its microbial agent can reduce the application of chemical fertilizers and pesticides in the strawberry production process, avoiding soil compaction, organic matter decline, environmental pollution, ecological imbalance, drug residues, food safety and other problems caused by excessive use of chemical fertilizers and pesticides , with good ecological and social benefits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a novel methylotrophic Bacillus Z-1 strain with antagonistic effect on strawberry wilt pathogens and a preparation method and application of a microbial agent containing the strain. Background technique [0002] Methylotrophic Bacillus Z-1 (Bacillus methylotrophicus Z-1) in taxonomy belongs to Cellular organisms, Bacteria, Firmicutes, Bacilli, Bacillus Bacillales, Bacillaceae, and Bacillus are widely distributed in soil and other natural environments, and are one of the dominant species in soil and plant microecology. In recent years, with the continuous deepening of the research on methylotrophic bacillus, it has been found that methylotrophic bacillus has various biological activities such as insecticidal, antibacterial, and biodegradable, and is considered to be the most biological control in the genus Bacillus. One of the strains with application value. [0003] Strawberry (Fra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 徐永平周通李晓宇陈岩王丽丽张喆王思佳
Owner DALIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com