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Human placenta mesenchymal stem cell suspension protection agent

A technology of stem cells and human placenta, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of rapid decline in cell activity, lack of nutrition and buffer capacity, and easy aggregation of cells to achieve maintenance The effect of stem cell activity, large-scale production, and uniform cell shape

Active Publication Date: 2018-06-22
GENESIS STEMCELL REGENERATIVE MEDICINE ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physiological saline or glucose injection is used as the infusion medium of cells. Due to its lack of nutrition and buffer capacity, the cell activity declines very quickly, and the cells are easy to aggregate, so there is a safety hazard in intravenous infusion.
Plasma can maintain cell activity well, but plasma contains a large number of unknown components, allogeneic infusion has potential safety hazards, and homologous infusion is extremely costly

Method used

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  • Human placenta mesenchymal stem cell suspension protection agent
  • Human placenta mesenchymal stem cell suspension protection agent
  • Human placenta mesenchymal stem cell suspension protection agent

Examples

Experimental program
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Effect test

preparation example Construction

[0020] (1) Preparation of the base liquid: Dilute and mix the dextran sodium chloride injection and the NaCl solution for injection at a ratio of 1:1 to complete the preparation of the base liquid. The concentration of the dextran sodium chloride injection is 6%. The concentration of NaCl solution is 0.9%;

[0021] (2) Addition of ingredients: add human serum albumin, trehalose and vitamin C to the above base liquid in sequence, the amount of human serum albumin is 2-10%, the amount of trehalose is 0.001-0.005%, vitamin The amount of C added is 0.01-0.05%, fully oscillated and mixed, the oscillating time is 30 s-1 min, and the oscillating speed is 1820 rpm, and the human placental mesenchymal stem cell suspension protective agent is prepared;

[0022] (3) Storage: Place the prepared human placental mesenchymal stem cell suspension protective agent in a 500 ml sterile medium bottle and store it at 4°C.

Embodiment 1

[0024] Preparation of placental stem cells

[0025] (1) Collection of human placenta tissue: the placenta was taken from a full-term healthy pregnant woman whose HBV antigen, anti-HCV antibody, anti-HIV antibody, anti-Treponema pallidum antibody, ALT, mycoplasma and other test items were all negative, and the placenta was removed from the operating table Afterwards, immersed in a special placenta preservation solution, maintained at 2-8°C for transportation, and separated within 24 hours;

[0026] (2) Tissue disinfection and washing: Take the placenta out of the preservation solution, place it flat on a sterile tray with the umbilical cord facing upward, cut off the umbilical cord, peel off the amniotic membrane, cut the chorionic tissue 0-0.3 cm thick under the amniotic membrane, and put Put it into a sterile petri dish, peel off the decidua basalis tissue, cut off the terminal villi clusters, and wash twice with 0.9% normal saline;

[0027] (3) Enzyme digestion: put the cho...

Embodiment 2

[0037] A kind of human placenta mesenchymal stem cell suspension protection agent

[0038]Take 6% low-molecular-weight dextran sodium chloride injection and 0.9% NaCl solution for injection, dilute and mix them 1:1, then use it as the base solution, add human serum albumin, trehalose, vitamin C, and human serum albumin into the base solution The volume ratio of trehalose is 2%, the mass percentage of trehalose is 0.001%, and the mass percentage of vitamin C is 0.01%. After fully mixing, the cell protection agent is prepared, and the prepared cell protection agent is placed in a 500 ml sterile medium bottle. Store at 4°C.

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Abstract

The invention discloses a human placenta mesenchymal stem cell suspension protection agent, which is prepared from the following ingredients including human serum albumin, mycose, vitamin C, dextran sodium chloride injection and NaCl solution for injection. The preparation of the human placenta mesenchymal stem cell suspension protection agent is completed through the steps of basic solution preparation, ingredient addition and storage. The activity, the dryness performance and the uniformity of the placenta mesenchymal stem cells can be effectively maintained; the ingredients are specific; the different source animal serum is not contained; the safety is high; in addition, the storage method provided by the invention can effectively maintain the stem cell activity; high efficiency and time saving are realized; the scaled production is facilitated; experiments show that after the human placenta mesenchymal stem cells are stored in the cell storage agent for 48h at 4 to 8 DEG C; the cellular morphology is uniform; the survival rate is still maintained at a value being 90 percent or higher; the mesenchymal stem cell specific surface mark CD73, CD105 and CD90 expression rate is higherthan 95 percent.

Description

technical field [0001] The invention relates to a stem cell suspension protection solution, in particular to a human placenta mesenchymal stem cell suspension protection agent. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of primitive cells with self-renewal ability and multilineage differentiation potential under suitable microenvironment. It is first found in bone marrow and subsequently isolated in fat, bone, muscle, lung, liver, pancreas, and in umbilical cord, cord blood, and placental tissue. Because of its strong proliferation ability, strong differentiation ability, low immunogenicity and immune regulation function, its clinical application value has attracted more and more attention. [0003] The chorion is derived from the placental tissue of pregnant women after childbirth and is the main component of the placenta. The chorionic layer tissue is rich in a large number of mesenchymal stem cells and vascular endothelial cells. Studies hav...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 谢再东王军霞潘春雷殷鉴强刘定生
Owner GENESIS STEMCELL REGENERATIVE MEDICINE ENG CO LTD
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