Human placenta mesenchymal stem cell suspension protection agent

A technology of stem cells and human placenta, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of rapid decline in cell activity, lack of nutrition and buffer capacity, and easy aggregation of cells to achieve maintenance The effect of stem cell activity, large-scale production, and uniform cell shape
CN108184818AActive Publication Date: 2018-06-22GENESIS STEMCELL REGENERATIVE MEDICINE ENG CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GENESIS STEMCELL REGENERATIVE MEDICINE ENG CO LTD
Publication Date
2018-06-22

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Abstract

The invention discloses a human placenta mesenchymal stem cell suspension protection agent, which is prepared from the following ingredients including human serum albumin, mycose, vitamin C, dextran sodium chloride injection and NaCl solution for injection. The preparation of the human placenta mesenchymal stem cell suspension protection agent is completed through the steps of basic solution preparation, ingredient addition and storage. The activity, the dryness performance and the uniformity of the placenta mesenchymal stem cells can be effectively maintained; the ingredients are specific; the different source animal serum is not contained; the safety is high; in addition, the storage method provided by the invention can effectively maintain the stem cell activity; high efficiency and time saving are realized; the scaled production is facilitated; experiments show that after the human placenta mesenchymal stem cells are stored in the cell storage agent for 48h at 4 to 8 DEG C; the cellular morphology is uniform; the survival rate is still maintained at a value being 90 percent or higher; the mesenchymal stem cell specific surface mark CD73, CD105 and CD90 expression rate is higherthan 95 percent.
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Description

technical field

[0001] The invention relates to a stem cell suspension protection solution, in particular to a human placenta mesenchymal stem cell suspension protection agent. Background technique

[0002] Mesenchymal stem cells (MSCs) are a type of primitive cells with self-renewal ability and multilineage differentiation potential under suitable microenvironment. It is first found in bone marrow and subsequently isolated in fat, bone, muscle, lung, liver, pancreas, and in umbilical cord, cord blood, and placental tissue. Because of its strong proliferation ability, strong differentiation ability, low immunogenicity and immune regulation function, its clinical application value has attracted more and more attention.

[0003] The chorion is derived from the placental tissue of pregnant women after childbirth and is the main component of the placenta. The chorionic layer tissue is rich in a large number of mesenchymal stem cells and vascular endothelial cells. Studies hav...

Claims

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