Desilicication-digestion two-stage type bioleaching method for radioactive elements in red mud
A radioactive element and biological leaching technology, applied in the removal of solid waste, etc., can solve problems such as reducing the efficiency of biological leaching, and achieve the effect of reducing costs, saving processing costs, and saving high-temperature sterilization costs.
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[0041] The preparation method of Bacillus mucilaginosus activation solution: inoculate Bacillus mucilaginosus in the sterilized activation culture medium and culture at 30°C for 3 days. Activation medium components (all by weight): sucrose 2%, yeast extract 0.2%, MgCl 2 0.02%, CaCO 3 0.01%, Fe 2 (SO 4 ) 3 0.005%, the solvent is deionized water.
[0042] The sterilization conditions of the desiliconization medium and the activation solution of Bacillus glaciens were sterilization at 121°C for 20 minutes.
[0043] (4). The mycelium pellets obtained by the membrane filtration method in step (2) are added to the desilicated medium treated in step (3), so that the organic acid produced by the physical contact of the mycelial pellets and the metabolism of the bacteria The radioactive elements in silicate minerals such as zircon can be leached into the leaching solution. The added amount of mycelium pellets is 1 to 5% of the weight of the sterilized desilication medium (without silica...
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[0046] 1. Mix the red mud and water uniformly to make a slurry, enrich and separate the silicate minerals mainly zircon in the red mud by flotation. The composition of the flotation agent (all by weight): 0.24% of oleic acid, 0.28% of oxidized paraffin soap, and 0.32% of rosin oil.
[0047] 2. Prepare the leaching medium, the composition of the leaching medium (all by weight): 20% sucrose, 0.1% yeast extract, 0.1% peptone, and deionized water as the solvent. Place 1L of leaching medium in a 5L beaker, and sterilize in an autoclave at 121°C for 20 minutes. After the leaching medium is cooled, 10 mL of Aspergillus niger spore suspension is inoculated. Place the culture medium on a shaker and incubate at 32°C and 120 rpm for 72 hours. After the cultivation is completed, filter the medium containing the mycelial pellets in a vacuum filter to obtain the mycelial pellets and the sterilized culture solution, which are respectively placed in the freezer for later use.
[0048] 3. Prepar...
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