A combined degradation method of zearalenone and ochratoxin a in a solution

A technology for zearalenone and ochratoxin, which is applied in the preparation of test samples, instruments, measuring devices, etc., to achieve the effects of remarkable effect, efficient degradation and simple operation

Active Publication Date: 2020-10-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention aims at the simultaneous existence of ZEN and OTA in food mycotoxin pollution, and proposes to use electron beam irradiation to jointly degrade ZEN in the solution by optimizing the parameters of toxin standards in different solvents, different concentration ratios and different irradiation doses. and a theoretical approach to OTA

Method used

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  • A combined degradation method of zearalenone and ochratoxin a in a solution
  • A combined degradation method of zearalenone and ochratoxin a in a solution
  • A combined degradation method of zearalenone and ochratoxin a in a solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1, the influence of different concentration ratios on the irradiation effect of ZEN and OTA mixed solution

[0021] At room temperature, a certain amount of ZEN and OTA standard stock solutions were mixed, blown to dryness with nitrogen, and redissolved in acetonitrile to obtain ZEN / OTA concentration ratios of 5.0 / 0.1, 2.0 / 0.2, 1.0 / 0.5 and 0.5 / 1.0μg / mL working solution is irradiated with 9kGy electron beam.

[0022] Result analysis:

[0023] Such as figure 1 As shown, under different concentration ratios, both ZEN and OTA in the working solution have achieved significant degradation effects. The OTA degradation rate is over 90%, and the ZEN degradation rate is also higher than 65%. The concentration ratio is 1.0 / 0.5μg / mL When , the comprehensive degradation effect of ZEN and OTA in the working solution was slightly higher than that of other groups.

Embodiment 2

[0024] Embodiment 2, the influence of different solvents on the irradiation effect of ZEN and OTA mixed solution

[0025] At room temperature, a certain amount of ZEN and OTA standard stock solutions were mixed, blown to dryness with nitrogen, and redissolved in acetonitrile and methanol respectively to obtain a working solution with a ZEN / OTA concentration ratio of 1.0 / 0.5 μg / mL, using 9 kGy electron beam irradiation treatment.

[0026] Result analysis:

[0027] Such as figure 2 As shown, ZEN and OTA in different solvent working solutions have achieved significant degradation results, and in acetonitrile solvent, the degradation rates of OTA and ZEN are as high as 90.61% and 74.67%. The reason is that methanol is a hole-scavenging agent, which can capture hydroxyl radicals and hydrated electrons, and has a certain inhibitory effect on the degradation of toxins. Acetonitrile solvents are irradiated at this dose to produce a certain amount of free radicals, which participate...

Embodiment 3

[0028] Embodiment 3, the impact of different irradiation doses on the irradiation effect of ZEN and OTA mixed solution

[0029] At room temperature, take a certain amount of ZEN and OTA standard stock solutions and mix them respectively. After nitrogen blowing to dryness, use acetonitrile to redissolve to obtain a working solution with a ZEN / OTA concentration ratio of 1.0 / 0.5 μg / mL. Use 3-12 kGy Electron beam irradiation treatment.

[0030] Result analysis:

[0031] Such as image 3 As shown, at a dose of 3kGy, the degradation rates of ZEN and OTA are basically the same, and as the irradiation dose increases, the degradation rate of OTA is gradually higher than that of ZEN. 86.70%. The reason is that the molecular structure of OTA contains relatively more polar sites, which is easy to be degraded by electron beam irradiation. After 9kGy, the degradation rate of ZEN shows a certain increase. The reason is that at a higher irradiation dose, acetonitrile produces free radicals...

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Abstract

The invention discloses a combined degradation method of zearalenone and ochratoxin A in a solution, and belongs to the technical field of the degradation of fungaltoxin in food and the environmentalprotection. The invention is the novel technology in the fungaltoxin combined subduction loop in the crops of grain and the like, the feature is that an electronic beam produced by an electronic accelerator is used for performing radiation degradation on the mixed solution of the ZEN and the OTA. The experimental process verifies that the effect of degrading the ZEN and OTA in the mixed solution by the electronic beam is obvious; the concentrations of the ZEN and the OTA in the mixed solution is 1.0 and 0.5 microgram / mL, the radiation degradation effect is the most obvious, the degradation rate of the ZEN and OTA in the acetonitrile solution at 12kGy is 86.70% and 93.35%. That means that the electronic beam radiation changes or eliminates the toxicity sites in the ZEN and OTA molecules, and the aim of efficient degradation is achieved.

Description

technical field [0001] The invention relates to a method for simultaneously degrading zearalenone and ochratoxin A in a solution by electron beam irradiation, and belongs to the technical field of degrading mycotoxins in food and environmental protection. Background technique [0002] Zearalenone (Zearalenone, ZEN) and ochratoxin A (Ochratoxin A, OTA) are toxic secondary metabolites produced by Fusarium, Aspergillus, and Penicillium, respectively. A large number of human and animal safety accidents and economic and environmental losses. In literature reports on mycotoxin contamination of food crops such as corn, barley, and wheat, ZEN and OTA mostly co-exist. Research data show that both ZEN and OTA have strong immunotoxicity. In addition, ZEN also has certain genotoxicity, reproductive toxicity and cytotoxicity. OTA also has strong kidney and liver toxicity, which can cause teratogenicity, carcinogenesis and Mutagenic. [0003] There are many existing ZEN and OTA degrada...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/44G01N30/02
CPCG01N1/44G01N30/02
Inventor 罗小虎陈正行李克王韧王莉李亚男杜志红潘丽红
Owner JIANGNAN UNIV
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