Method for separating and purifying algae species of euglenophyta

A technology for separation and purification of euglena liquid, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and separation of microorganisms. It can solve the problems of easy-to-contaminate bacteria and achieve the effect of facilitating and maintaining stable traits

Active Publication Date: 2018-07-13
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the long screening time of euglena species and the problem that it is very easy to infect bacteria in the process of subculture and large-scale cultivation, the present invention provides a low-cost and easy-to-operate algae purification procedure by using measures such as acidification and light induction, so that To achieve the purpose of quickly obtaining pure species of euglena and adapting to large-scale euglena production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Natural water samples. Sampling in the field shows that Euglena cells grow in large numbers in a certain water area, which is the dominant population. A 500 mL sample was taken from the water.

[0037] (1) Return to the laboratory, add the water sample to the sterile triangular flask, and insert the nutrient salt, the formula is: ammonium nitrate 1g / L, potassium dihydrogen phosphate 0.2g / L, magnesium sulfate 0.1g / L, calcium chloride 0.1g / L, sodium citrate 1g / L, VB 1 10μg / L, VB 12 0.1μg / L, ferric chloride 0.588mg / L, manganese chloride 0.108mg / L, zinc sulfate 0.078mg / L, cobalt chloride 0.012mg / L, sodium molybdate 0.0075mg / L; adjust the above with 1M HCl The pH of the algae solution is 3.5, the light intensity is 3000lx, the light is 12h / d, and the CO 2 5% mixed gas (0.3vvm) was used for cultivation, the cultivation temperature was 25±2°C, and the cultivation time was 3 days.

[0038] (2) Take samples for microscopic examination and preliminarily estimate the concen...

Embodiment 2

[0046] Natural water samples. Sampling in the field shows that there are euglena cells in a certain water area, and euglena cells, some green algae and diatoms are the dominant algal strains. A 500 mL sample was taken from the water.

[0047] (1) Return to the laboratory, add the water sample to the sterile Erlenmeyer flask, and insert nutrient salts: ammonium nitrate 1g / L, potassium dihydrogen phosphate 0.2g / L, magnesium sulfate 0.1g / L, calcium chloride 0.1g / L L, sodium citrate 1g / L, VB 1 10μg / L, VB 12 0.1μg / L, ferric chloride 0.588mg / L, manganese chloride 0.108mg / L, zinc sulfate 0.078mg / L, cobalt chloride 0.012mg / L, sodium molybdate 0.0075mg / L; adjust the above with 1M HCl The pH of the algae solution is 3.5, the light intensity is 3000lx, the light is 12h / d, and the CO 2 5% mixed gas (0.3vvm) was used for cultivation, the cultivation temperature was 25±2°C, and the cultivation time was 3 days. Sampling and microscopic inspection found that there were still many green...

Embodiment 3

[0055] Natural water samples. Sampling in the field shows that there are euglena cells in a certain water area, but the dominant algae species are green algae and diatoms. A 500 mL sample was taken from the water.

[0056] (1) Return to the laboratory, add the water sample to the sterile Erlenmeyer flask, and insert nutrient salts: ammonium nitrate 1g / L, potassium dihydrogen phosphate 0.2g / L, magnesium sulfate 0.1g / L, calcium chloride 0.1g / L L, sodium citrate 1g / L, VB 1 10μg / L, VB 12 0.1μg / L, ferric chloride 0.588mg / L, manganese chloride 0.108mg / L, zinc sulfate 0.078mg / L, cobalt chloride 0.012mg / L, sodium molybdate 0.0075mg / L; adjust the above with 1M HCl The pH of the algae solution is 3.5, the light intensity is 3000lx, the light is 12h / d, and the CO 2 5% mixed gas (0.3vvm) was used for cultivation, the cultivation temperature was 25±2°C, and the cultivation time was 3 days. Sampling and microscopic inspection found that there were still many green algae and diatoms, ...

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Abstract

The invention discloses a method for separating and purifying algae species of euglenophyta. The method comprises the following steps: selecting a water sample containing euglenophyta cells or an euglenophyta algae solution containing infectious microbes, adding a certain amount of inorganic culture medium suitable for growth of the euglenophyta, then regulating the pH (Potential of Hydrogen) of the algae solution to 3 to 4, and culturing for 2 to 7 days under the light intensity which is 3000 to 30000 lx; then, diluting the algae solution, putting the diluted algae solution in a specific container, applying illumination of which the light intensity is 2000 to 5000 lx to certain parts of a culture device for a certain time, sucking a certain amount of algae solution in an illumination part, and inoculating into a culture porous plate for culturing. Through the way, higher-purity algae species can be obtained within a short time, the growth period is shortened, and the success probability of large-scale growth is increased.

Description

technical field [0001] The invention relates to the technical field of purification of microalgae species, in particular to a method for separating and purifying euglena species. Background technique [0002] Euglena lives in fresh water and often forms green algae blooms in lakes and ditches, especially in waters rich in organic matter. It forms a green water film on the water surface, which forms or disperses with changes in external light and other conditions. Euglena has no cell wall, has a long flagella, and moves through flagella and cell twisting. There are chloroplasts in the cells, which can rely on photosynthesis for autotrophy, and can also use organic matter (such as glucose, glutamic acid, malic acid, pyruvic acid) , lactic acid, and ethanol) are heterotrophic, and phototrophic and heterotrophic are usually carried out simultaneously in nature. Euglena is long rod-shaped, generally 10-20 μm in width, and up to 100 μm in length; it has one eye point, which is mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/02C12N1/12
Inventor 王兆伟彭小伟王六伟
Owner NINGBO FUTIAN BIOTECH
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