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Method used for measuring chitosan content through dual-wavelength resonance Rayleigh scattering

A technology of resonance Rayleigh scattering and chitosan, which is applied in the field of macromolecular detection, can solve problems such as difficult quantification and cumbersome operation techniques, and achieve the effects of simple operation, strong anti-interference ability, and cheap reagents

Active Publication Date: 2018-07-13
GUANGDONG PHARMA UNIV
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Problems solved by technology

[0006] In order to overcome the technical deficiencies in the prior art that the content of chitosan in the finished product is difficult to quantify and the operation technology is cumbersome, the invention provides a method for measuring the content of chitosan by a dual-wavelength resonance Rayleigh scattering method. , Brilliant blue dye combines with chitosan to form an ion association that can significantly enhance the resonance Rayleigh scattering signal in the system, and two characteristic peaks appear at 345nm and 445nm

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  • Method used for measuring chitosan content through dual-wavelength resonance Rayleigh scattering
  • Method used for measuring chitosan content through dual-wavelength resonance Rayleigh scattering
  • Method used for measuring chitosan content through dual-wavelength resonance Rayleigh scattering

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Embodiment

[0058] A kind of method that dual-wavelength resonance Rayleigh scattering method measures chitosan content, it comprises the steps:

[0059] 1) Draw the standard curve of the chitosan concentration of ΔI and different molecular weights

[0060] Add 1.0mL chitosan standard solution with a certain concentration gradient to the 10mL colorimetric tube, 1.0mL pH=3.0 citric acid-sodium hydrogen phosphate buffer solution, 1.0mL concentration is 1.0×10 -3 mol / L bright blue solution, use triple distilled water to make up to the scale, and shake well, put it at room temperature for 10 minutes, take 1ml of the mixed solution and add it to a quartz cuvette, and put it on the F-2500 fluorescence spectrophotometer. lambda ex =λ em Carry out synchronous scanning, record respectively the resonance scattering intensity value I of each detection system containing chitosan at the maximum resonance Rayleigh scattering wavelength λ=345nm place system 1 , where the resonance scattering intensit...

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Abstract

The invention relates to a method used for measuring chitosan content through dual-wavelength resonance Rayleigh scattering, and belongs to the field of large molecule detection. The method is capableof avoiding technical defects in the prior art that quantitative detection of the content of chitosan of finished products is difficult to realize, and operation technology is complex. According to the method, at certain conditions, brilliant blue dye is combined with chitosan so as to form an ionic associate capable of enhancing resonance Rayleigh scattering signals in a system, and two characteristic peaks at 345nm and 445nm are observed. Under preset conditions, when the concentration of chitosan ranges from 0.005 to 1.8<mu>g / ml, the linear relationship of the concentration of chitosan with resonance Rayleigh scattering strength is excellent, so that the dual-wavelength resonance Rayleigh scattering method used for measuring chitosan content is established. The dual-wavelength resonance Rayleigh scattering is influenced by the molecular weight of chitosan; the adopted reagents are cheap; the sensitivity, the reproducibility, and the anti-interference capacity are high, and operation is convenient.

Description

technical field [0001] The invention relates to a method for measuring chitosan content by a dual-wavelength resonance Rayleigh scattering method, belonging to the field of macromolecule detection. Background technique [0002] Chitin, also known as chitin, is a polysaccharide linked by N-acetyl-2-amino-2-deoxy-D-glucose in the form of β-1,4 glycosidic bonds. It widely exists in the shells of crustaceans such as shrimps, crabs and insects, the cells of lower plant fungi and algae in nature, and the cell walls of higher plants. The annual biosynthesis of chitin is as high as 100 billion tons, and its stock is second only to cellulose in nature. [0003] Chitosan (chitosan, CTS) is a substance produced by the deacetylation reaction of the linear amino polysaccharide chitin. There are many amino groups, hydroxyl groups and deacetylation groups distributed on its molecular chain, and its molecular weight ranges from hundreds of thousands to several million. Various intramolecu...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/643G01N2021/6421
Inventor 白研苏政权
Owner GUANGDONG PHARMA UNIV
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