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Application of bs1-ct protein in regulation of plant cell wall xylan deacetylation

A plant cell, sugar acetylation technology, applied in the application field of BS1-CT protein in regulating plant cell wall xylan deacetylation reaction, can solve the problems of affecting the fermentation process and increasing the cost of ethanol, and achieve important economic value and reduce The effect of production costs

Active Publication Date: 2020-06-09
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the process of converting biomass resources into bioenergy ethanol, microbial fermentation is required, and the acetyl groups released from plant cell wall polysaccharides lead to environmental acidification and affect the fermentation process, while optimizing conditions increases the cost of ethanol production

Method used

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  • Application of bs1-ct protein in regulation of plant cell wall xylan deacetylation
  • Application of bs1-ct protein in regulation of plant cell wall xylan deacetylation
  • Application of bs1-ct protein in regulation of plant cell wall xylan deacetylation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1, the acquisition of rice brittle sheath mutant and BS1-CT protein

[0081] 1. Obtaining rice brittle sheath mutant and its BS1 protein sequence analysis

[0082] 1. Phenotype of rice brittle sheath mutant

[0083] The rice brittle sheath mutant brittle sheath1 (abbreviated as bs1 or mutant bs1) is a spontaneous mutation material of the japonica rice variety "Jinjinqing". Compared with the japonica rice variety "Jinjinqing", the mutant bs1 showed the following main manifestations: (1) the leaf sheath became brittle (the mechanical strength of the leaf sheath decreased significantly, and the xylem vessel structure was abnormal); (2) the plant became shorter.

[0084] 2. Sequence analysis of BS1 protein in rice brittle sheath mutant

[0085] It was shown by sequencing that compared with the wild-type rice plant (Jinjinqing), only the first base of the second intron of the BS1 gene was mutated from G to A from the 5' end of the brittle sheath mutant of rice, ...

Embodiment 2

[0098] Embodiment 2, the preparation of BS1-CT protein

[0099] 1. Construction of recombinant plasmids

[0100] 1. Extract the total RNA of the japonica rice variety "Jinjinqing" and reverse transcribe it into cDNA.

[0101]2. Using the cDNA extracted in step 1 as a template, perform PCR amplification with a primer pair composed of F2 and R2, recover the PCR amplification product, and connect it to the T vector.

[0102] F2: 5'-TCTCGAGAAGAGAGAGGCTGAAGCAGAGGGGAAGGTGAACGGGA-3';

[0103] R2: 5'-TTCTAGACCTGAAGATTGGAAGATCGGTTGG-3'.

[0104] 3. Digest the T vector in step 2 with restriction endonucleases XhoI and XbaI, and recover the digested product.

[0105] 4. Digest the pPICZαC vector with restriction endonucleases XhoI and XbaI to recover a vector backbone of about 3600 bp.

[0106] 5. Ligate the digested product of step 3 with the vector backbone of step 4 to obtain the recombinant plasmid pPICZαC-BS1-CT. According to the sequencing results, the structure of the recombi...

Embodiment 3

[0115] Example 3. Application of fusion protein BS1-CT-His in regulating xylan deacetylation reaction in vitro

[0116] 1. Substrate specificity of fusion protein BS1-CT-His to acetylated monosaccharides

[0117] 1. Acetylated monosaccharide samples

[0118] Acetylated monosaccharide samples are as follows: 1,2,3,4,6-5-O-acetyl-β-D-glucose (Glc) (Beijing Kaisenlai Pharmaceutical Technology Co., Ltd., 604-69-3); 1 ,2,3,4,6-5-O-acetyl-β-D-galactopyranose (Gal) (Beijing Kaisenlai Pharmaceutical Technology Co., Ltd., 4163-60-4); 1,2,3 ,5-4-O-acetyl-α-L-arabinofuranose (Ara) (Tianjin Xiensi Biochemical Technology Co., Ltd., 79120-81-3); 1,2,3,4,6-5-O -Acetyl-β-D-mannopyranose (Man) (Beijing Kaisenlai Pharmaceutical Technology Co., Ltd., 4026-35-1); Methyl 2,3,4-3-O-acetyl-β-D - Xylose (Xyl) (Beijing Kaisenlai Pharmaceutical Technology Co., Ltd., 13007-37-9) (abbreviated as triacetylmethylxylose).

[0119] 2. Determination of the activity of the fusion protein BS1-CT-His on acet...

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Abstract

The invention discloses the application of BS1-CT protein in regulating the deacetylation reaction of plant cell wall xylan. The BS1-CT protein is the protein of the following a) or b) or c): a) the amino acid sequence is the protein shown at position 25-382 of SEQ ID NO: 1; b) the N of the protein shown at position 25-382 of SEQ ID NO: 1 A fusion protein obtained by linking a tag at the terminal and / or C-terminal; c) a protein obtained by substituting and / or deleting and / or adding one or several amino acid residues to the amino acid sequence shown at positions 25-382 of SEQ ID NO: 1. It is proved by experiments that the BS1-CT protein of the present invention has the function of catalyzing the deacetylation reaction of plant cell wall xylan, can be used to regulate the acetylation modification level of plant cell wall xylan, and will play a role in the process of converting biomass resources into energy. It plays an important role in reducing the production cost of bioenergy and has important economic value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of BS1-CT protein in regulating the deacetylation reaction of plant cell wall xylan. Background technique [0002] O-acetylation modification is a common modification form in plant cell wall polysaccharides. With the exception of cellulose, β-1,3-1,4-linked glucans and some glycoproteins, O-acetylation modifications are present on almost all other cell wall polysaccharides. [0003] O-acetylation modification may affect the cell wall structure by affecting the physiological and biochemical properties of cell wall polysaccharides. Studies have found that acetylation affects the interaction of polysaccharides with polar molecules. The pattern of acetylation modification on xylan affects the way xylan binds to cellulose, which in turn affects the structure of the cell wall. The degree of O-acetylation modification of plant cell wall polysaccharides varies ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8246C07K14/00C12N9/18C12N15/82
Inventor 周奕华张保才张兰军
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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