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A preparation method of graphene oxide/nano hydroxyapatite composite silica gel modified porous scaffold material

A nano-hydroxyapatite and porous scaffold technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of poor binding of gelatin and graphene oxide, poor mechanical properties of scaffolds, complicated preparation process, etc. Achieve good in vitro biomineralization performance, increase biological performance, cytotoxicity and non-toxicity

Active Publication Date: 2020-04-07
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some researchers used gelatin cross-linking modification to prepare porous scaffold materials. Although scaffold materials with better biological properties can be obtained, gelatin and graphene oxide are not well bonded, resulting in poor mechanical properties of the scaffold. Poor degradability in the medium, and the preparation process is more complicated, and the practicability is not high

Method used

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  • A preparation method of graphene oxide/nano hydroxyapatite composite silica gel modified porous scaffold material
  • A preparation method of graphene oxide/nano hydroxyapatite composite silica gel modified porous scaffold material
  • A preparation method of graphene oxide/nano hydroxyapatite composite silica gel modified porous scaffold material

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Embodiment 1

[0030] A preparation method of graphene oxide / nano hydroxyapatite composite silica gel modified porous scaffold material, specifically comprising the following steps:

[0031] (1) Add 1.25g of calcium nitrate to a 200mL beaker of distilled water to make a 0.038mol / L calcium nitrate solution, place it on a magnetic stirrer and stir continuously, and the speed of the magnetic stirrer is 250 rpm , to completely dissolve the raw materials; then heat in a water bath at a heating rate of 3°C / min and control the solution temperature to 37°C, then add 0.282g of graphene oxide powder, continue stirring to disperse evenly, and obtain solution A;

[0032] (2) Use a graduated cylinder to measure ammonia water and add it to deionized water to make a concentration of 0.025mol / L ammonia solution. Use ammonia solution to adjust the pH of solution A to 10; add diammonium hydrogen phosphate to 100mL of distilled water to make 0.0455mol / L of diammonium hydrogen phosphate solution, stirred to di...

Embodiment 2

[0039] A preparation method of graphene oxide / nano hydroxyapatite composite silica gel modified porous scaffold material, the specific operations are as follows:

[0040](1) Add 1.47g of calcium nitrate to a 200mL beaker of distilled water to make a 0.045mol / L calcium nitrate solution, place it on a magnetic stirrer and stir continuously, and the speed of the magnetic stirrer is 250 rpm , the raw material is completely dissolved; then heated in a water bath with a heating rate of 4°C / min and controlled solution temperature is 37°C, then add 0.282g graphene oxide powder, continue to stir to make it evenly dispersed, and obtain solution A;

[0041] (2) Use a graduated cylinder to measure ammonia water and add it to deionized water to make a concentration of 0.025mol / L ammonia solution. Use ammonia solution to adjust the pH of solution A to 9.5; add diammonium hydrogen phosphate to 100mL of distilled water to make 0.054mol / L of diammonium hydrogen phosphate solution, stirred to ...

Embodiment 3

[0047] A preparation method of graphene oxide / nano hydroxyapatite composite silica gel modified porous scaffold material, the specific operations are as follows:

[0048] (1) Add 2.448g of calcium sulfate to a 200mL distilled water beaker to make it into a 0.045mol / L calcium sulfate solution, place it on a magnetic stirrer and stir continuously, and the speed of the magnetic stirrer is 300 rpm , to completely dissolve the raw material; then heat in a water bath at a heating rate of 5°C / min and control the solution temperature to 30°C, then add 0.897g of graphene oxide powder, continue stirring to disperse evenly, and obtain solution A;

[0049] (2) Use a measuring cylinder to measure ammonia water and add it to deionized water to make a concentration of 0.030mol / L ammonia solution. Use ammonia solution to adjust the pH of solution A to 10.5; add diammonium hydrogen phosphate to 100mL of distilled water to make 0.054mol / L of diammonium hydrogen phosphate solution, stirred to d...

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Abstract

The invention discloses a preparation method of a graphene oxide / nano hydroxyapatite composite silica gel modified porous scaffold material, and belongs to the technical field of biomaterials. The preparation method comprises the following steps of firstly, using graphene oxide, a calcium source and a phosphorus source as raw materials, preparing a graphene oxide / nano hydroxyapatite composite powder material through an ion simultaneous titration method; afterwards, adding silica gel and a pore-forming agent, extrusion-forming through a mold to obtain a scaffold material, subsequently, placinga scaffold into alcohol to soak, and sintering in an inert-atmosphere furnace, so as to finally obtain the porous scaffold material. The prepared porous scaffold material has ingredients and a porousstructure which are approach similar to those of the bone of a human body, has the pore sizes distributed from 300mum to 800mum, the porosity of 30 to 70 percent and the compressive strength reaching0.9Mpa to 4.2Mpa, is good in biodegradability and osteogenesis performance in simulated body fluid and further,and has no cytotoxicity, and the biodegradability and osteogenesis performance in simulated body fluid are good.

Description

technical field [0001] The invention relates to a preparation method of a graphene oxide / nano hydroxyapatite composite silica gel modified porous support material, which belongs to the technical application field of biomedical materials. Background technique [0002] As the main component of the human skeletal system, bone is responsible for important functions such as support, load bearing and movement. However, bone defects caused by diseases, trauma, aging, sports trauma and other reasons have become one of the clinical problems to be solved urgently. Tissue engineering materials are widely used as a good biological implant material. This scaffold material is implanted in the defect site. While the material is gradually degraded, the bone cells planted in the porous scaffold continue to proliferate, thereby achieving repair. The purpose of bone tissue defect. The ideal bone tissue scaffold material should meet the following points: (1) have good biocompatibility, the de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/08A61L27/02A61L27/50A61L27/56
CPCA61L27/025A61L27/08A61L27/12A61L27/50A61L27/56A61L2430/02
Inventor 陈庆华杨泽斌刘继涛颜廷亭
Owner KUNMING UNIV OF SCI & TECH