Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting antioxidant peptide from peony seed meal

A technology of peony seed meal and antioxidant peptides, which is applied in the field of extracting antioxidant peptides, can solve the problem of low activity of antioxidant peptides, achieve the effects of improving the level of comprehensive development and utilization, good market prospects, and high yield

Inactive Publication Date: 2018-07-31
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since there is not a simple linear relationship between the degree of hydrolysis and antioxidant capacity, the activity of antioxidant peptides prepared according to the degree of proteolysis is not very high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting antioxidant peptide from peony seed meal
  • Method for extracting antioxidant peptide from peony seed meal
  • Method for extracting antioxidant peptide from peony seed meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Freezing the peony seed meal at -20°C, thawing in microwave, degreasing, drying and grinding into powder to obtain peony seed meal powder for later use;

[0028] (2) Add peony seed meal powder to a mixed solution of deionized water and polyethylene glycol in a mass ratio of 1:25 by volume (the volume ratio of deionized water to polyethylene glycol is 1:0.1) and stir evenly to obtain a slurry Add 1.5% lactitol and 1% citric acid to the total weight of the slurry, mix well, let it stand for 15 minutes, then add 1mol / L NaOH solution to adjust the pH value to 11.0, 180 W ultrasonic 2.5 h, centrifuge The supernatant was then adjusted to pH 3.80 with 1 mol / L HCl solution, centrifuged to remove the supernatant to retain the precipitate, and the precipitate was placed in a vacuum freeze dryer to freeze-dry, and the freeze-dried powder was stored at -20°C for later use;

[0029] (3) Weigh 7 g of freeze-dried powder and dissolve it in 1000 mL of deionized water, add 0.35 g of...

Embodiment 2

[0032] (1) Freezing the peony seed meal at -20°C, thawing in microwave, degreasing, drying and grinding into powder to obtain peony seed meal powder for later use;

[0033] (2) Add peony seed meal powder to a mixed solution of deionized water and polyethylene glycol in a mass ratio of 1:25 by volume (the volume ratio of deionized water to polyethylene glycol is 1:0.2) and stir evenly to obtain a slurry Add 2% lactitol and 0.8% citric acid to the total weight of the slurry, mix well, let stand for 15 minutes, then add 1mol / L NaOH solution to adjust the pH value to 11.0, 180 W ultrasonic 2.5 h, centrifuge The supernatant was then adjusted to pH 3.80 with 1 mol / L HCl solution, centrifuged to remove the supernatant to retain the precipitate, and the precipitate was placed in a vacuum freeze dryer to freeze-dry, and the freeze-dried powder was stored at -20°C for later use;

[0034] (3) Weigh 7 g of the freeze-dried powder and dissolve it in 1000 mL of deionized water, add 0.56 g o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting antioxidant peptide from peony seed meal and belongs to the technical field of agriculture product processing. The method is realized by adopting the following specific steps: carrying out freezing and microwave thawing on the peony seed meal in sequence, degreasing, drying and grinding into powder; adding a solvent, lactitol and citric acid into the peony seed meal, allowing standing still and then adjusting the pH value to 11.0; carrying out ultrasonic treatment and centrifuging, and taking supernatant; then adjusting the pH value to 3.80, centrifuging, precipitating and freeze-drying to obtain lyophilized powder; weighing the lyophilized powder and dissolving the lyophilized powder into deionized water, adding beeswax and preheating to obtain a mixed solution; adjusting the pH value of the mixed solution to 8.0, adding protease, carrying out enzymolysis and enzyme denaturalixation, collecting the supernatant and then carrying out freeze-drying, thus obtaining a finished product. According to the method for extracting the antioxidant peptide from the peony seed meal, disclosed by the invention, the peony seed meal is utilized as araw material, and the hydrolysis degree and the antioxidant resistance are taken into full consideration, so that an optimum technology for polypeptide with higher antioxidant activity, which is prepared by utilizing peony seed meal protein, is obtained and provides a theoretical basis for deep processing, development and utilization of the peony seed meal; in addition, the preparation process issimple and the market prospect is good.

Description

technical field [0001] The invention relates to a method for extracting antioxidant peptides from peony seeds, belonging to the technical field of agricultural product processing. Background technique [0002] Bioactive peptides are peptides between proteins and amino acids, which have desirable biological activities, including antioxidant, hypotensive, antithrombotic, hypolipidemic, antimicrobial and anti-inflammatory effects. Antioxidant peptides are composed of 5-16 amino acid residues. Compared with other biomolecules, they have extremely strong biological activity and diversity, more significant antioxidant properties, and higher safety. The methods currently used to prepare antioxidant peptides mainly include artificial synthesis, enzymatic hydrolysis and biological fermentation. In the process of preparing biologically active peptides, enzymatic hydrolysis is the fastest, safest and most easy-to-control technology. It can simultaneously Improving the functional and b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K1/14A61P39/06
CPCC07K1/145C12P21/06
Inventor 孙金月阎震郭溆刘超程安玮王青王新坤
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com