Method for extracting antioxidant peptide from peony seed meal

A technology of peony seed meal and antioxidant peptides, which is applied in the field of extracting antioxidant peptides, can solve the problem of low activity of antioxidant peptides, achieve the effects of improving the level of comprehensive development and utilization, good market prospects, and high yield

Inactive Publication Date: 2018-07-31
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since there is not a simple linear relationship between the degree of hydrolysis and antioxidant capacit

Method used

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  • Method for extracting antioxidant peptide from peony seed meal
  • Method for extracting antioxidant peptide from peony seed meal
  • Method for extracting antioxidant peptide from peony seed meal

Examples

Experimental program
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Example Embodiment

[0026] Example 1

[0027] (1) Freeze the peony seed meal at -20°C, thawed it in a microwave, defatted, dry and grind it into a powder to obtain a peony seed meal for use;

[0028] (2) Add the peony seed meal powder to the mixed solution of deionized water and polyethylene glycol (the volume ratio of deionized water and polyethylene glycol is 1:0.1) according to the volume ratio of 1:25 mass ratio, and then the slurry will be obtained. Add 1.5% of the total weight of the slurry and 1% of citric acid. After mixing, let it stand for 15 minutes, then add 1mol / L NaOH solution to adjust the pH to 11.0, ultrasound at 180 W for 2.5 h, and centrifuge to take The supernatant is then adjusted to pH 3.80 with 1mol / L HCl solution, centrifuged to remove the supernatant to save the precipitate, the precipitate is placed in a vacuum freeze dryer to freeze-dry, and the freeze-dried powder is stored at -20°C for later use;

[0029] (3) Weigh 7g of the freeze-dried powder and dissolve it in 1000mL of ...

Example Embodiment

[0031] Example 2

[0032] (1) Freeze the peony seed meal at -20°C, thawed it in a microwave, defatted, dry and grind it into a powder to obtain a peony seed meal for use;

[0033] (2) Add the peony seed meal powder to the mixed solution of deionized water and polyethylene glycol (the volume ratio of deionized water and polyethylene glycol is 1:0.2) according to the volume ratio of 1:25 mass ratio, and then the slurry will be obtained. Add 2% of the total weight of the slurry and 0.8% citric acid. After mixing evenly, let it stand for 15 minutes, then add 1mol / L NaOH solution to adjust the pH to 11.0, ultrasound at 180 W for 2.5 h, and centrifuge to take The supernatant is then adjusted to pH 3.80 with 1mol / L HCl solution, centrifuged to remove the supernatant to save the precipitate, the precipitate is placed in a vacuum freeze dryer to freeze-dry, and the freeze-dried powder is stored at -20°C for later use;

[0034] (3) Weigh 7g of freeze-dried powder and dissolve it in 1000mL of ...

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Abstract

The invention relates to a method for extracting antioxidant peptide from peony seed meal and belongs to the technical field of agriculture product processing. The method is realized by adopting the following specific steps: carrying out freezing and microwave thawing on the peony seed meal in sequence, degreasing, drying and grinding into powder; adding a solvent, lactitol and citric acid into the peony seed meal, allowing standing still and then adjusting the pH value to 11.0; carrying out ultrasonic treatment and centrifuging, and taking supernatant; then adjusting the pH value to 3.80, centrifuging, precipitating and freeze-drying to obtain lyophilized powder; weighing the lyophilized powder and dissolving the lyophilized powder into deionized water, adding beeswax and preheating to obtain a mixed solution; adjusting the pH value of the mixed solution to 8.0, adding protease, carrying out enzymolysis and enzyme denaturalixation, collecting the supernatant and then carrying out freeze-drying, thus obtaining a finished product. According to the method for extracting the antioxidant peptide from the peony seed meal, disclosed by the invention, the peony seed meal is utilized as araw material, and the hydrolysis degree and the antioxidant resistance are taken into full consideration, so that an optimum technology for polypeptide with higher antioxidant activity, which is prepared by utilizing peony seed meal protein, is obtained and provides a theoretical basis for deep processing, development and utilization of the peony seed meal; in addition, the preparation process issimple and the market prospect is good.

Description

technical field [0001] The invention relates to a method for extracting antioxidant peptides from peony seeds, belonging to the technical field of agricultural product processing. Background technique [0002] Bioactive peptides are peptides between proteins and amino acids, which have desirable biological activities, including antioxidant, hypotensive, antithrombotic, hypolipidemic, antimicrobial and anti-inflammatory effects. Antioxidant peptides are composed of 5-16 amino acid residues. Compared with other biomolecules, they have extremely strong biological activity and diversity, more significant antioxidant properties, and higher safety. The methods currently used to prepare antioxidant peptides mainly include artificial synthesis, enzymatic hydrolysis and biological fermentation. In the process of preparing biologically active peptides, enzymatic hydrolysis is the fastest, safest and most easy-to-control technology. It can simultaneously Improving the functional and b...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14A61P39/06
CPCC07K1/145C12P21/06
Inventor 孙金月阎震郭溆刘超程安玮王青王新坤
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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