Method for extracting antioxidant peptide from peony seed meal
A technology of peony seed meal and antioxidant peptides, which is applied in the field of extracting antioxidant peptides, can solve the problem of low activity of antioxidant peptides, achieve the effects of improving the level of comprehensive development and utilization, good market prospects, and high yield
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[0026] Example 1
[0027] (1) Freeze the peony seed meal at -20°C, thawed it in a microwave, defatted, dry and grind it into a powder to obtain a peony seed meal for use;
[0028] (2) Add the peony seed meal powder to the mixed solution of deionized water and polyethylene glycol (the volume ratio of deionized water and polyethylene glycol is 1:0.1) according to the volume ratio of 1:25 mass ratio, and then the slurry will be obtained. Add 1.5% of the total weight of the slurry and 1% of citric acid. After mixing, let it stand for 15 minutes, then add 1mol / L NaOH solution to adjust the pH to 11.0, ultrasound at 180 W for 2.5 h, and centrifuge to take The supernatant is then adjusted to pH 3.80 with 1mol / L HCl solution, centrifuged to remove the supernatant to save the precipitate, the precipitate is placed in a vacuum freeze dryer to freeze-dry, and the freeze-dried powder is stored at -20°C for later use;
[0029] (3) Weigh 7g of the freeze-dried powder and dissolve it in 1000mL of ...
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[0031] Example 2
[0032] (1) Freeze the peony seed meal at -20°C, thawed it in a microwave, defatted, dry and grind it into a powder to obtain a peony seed meal for use;
[0033] (2) Add the peony seed meal powder to the mixed solution of deionized water and polyethylene glycol (the volume ratio of deionized water and polyethylene glycol is 1:0.2) according to the volume ratio of 1:25 mass ratio, and then the slurry will be obtained. Add 2% of the total weight of the slurry and 0.8% citric acid. After mixing evenly, let it stand for 15 minutes, then add 1mol / L NaOH solution to adjust the pH to 11.0, ultrasound at 180 W for 2.5 h, and centrifuge to take The supernatant is then adjusted to pH 3.80 with 1mol / L HCl solution, centrifuged to remove the supernatant to save the precipitate, the precipitate is placed in a vacuum freeze dryer to freeze-dry, and the freeze-dried powder is stored at -20°C for later use;
[0034] (3) Weigh 7g of freeze-dried powder and dissolve it in 1000mL of ...
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