Naphthoquinone compounds, preparation thereof and application to hypoglycemic product preparation

A compound and hypoglycemic technology are applied in the field of natural medicinal chemistry to achieve the effects of good hypoglycemic activity, novel structure and good medicinal and dietary effects

Inactive Publication Date: 2018-09-07
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on the functional effects of water chestnut mixed extracts, but few reports on single components. A single active component is iso

Method used

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  • Naphthoquinone compounds, preparation thereof and application to hypoglycemic product preparation
  • Naphthoquinone compounds, preparation thereof and application to hypoglycemic product preparation
  • Naphthoquinone compounds, preparation thereof and application to hypoglycemic product preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Grind dried water chestnuts (10.0 kilograms) into small pieces (0.8 centimeters), put them in a 92% ethanol solution and soak them for 16 hours, then carry out heat reflux extraction for 3 times, each time for 3 hours, the extracts are combined and Filtrate, and dry the filtrate at low temperature (53° C.) until it has no alcohol smell, and obtain the total extract of water chestnuts (1.05 kg);

[0045] (2) Suspend the total water chestnut extract in water (V / V=1:1), knead and dissolve (i.e. fully dissolve), let stand for 24 hours, filter to obtain the supernatant; Ester and n-butanol are extracted, and the solvent is evaporated to dryness to obtain cyclohexane extract (46.5 grams), ethyl acetate extract (126.2 grams) and n-butanol extract (258.6 grams);

[0046] (3) The extract obtained in step (2) is subjected to an activity screening experiment for inhibiting α-glucosidase (see the activity screening part of Example 4 for details), and the results show that the e...

Embodiment 2

[0055] (1) Crush the dried water chestnuts into small pieces (0.5 cm), place them in a 95% ethanol solution and soak them for 18 hours, then carry out heat reflux extraction for 3 times, each time for 2 hours, the extracts are combined and filtered, and the filtrate is low-temperature (55° C.) drying to no alcohol smell to obtain the total extract of water chestnuts;

[0056] (2) Suspend the total water chestnut extract in water (V / V=1:1), knead and dissolve (i.e. fully dissolve), let stand for 22 hours, filter to obtain the supernatant; Ester, n-butanol are extracted, and the solvent is evaporated to dryness to obtain cyclohexane extract, ethyl acetate extract and n-butanol extract respectively;

[0057] (3) Dissolve the ethyl acetate extract prepared in step (2) with petroleum ether, and carry out silica gel column chromatography (wet column, 100-200 mesh); use petroleum ether-ethyl acetate elution system according to petroleum ether The volume ratio of ether-ethyl acetate ...

Embodiment 3

[0062] (1) Crush the dried water chestnuts into small pieces (1.0 cm), put them in a 90% ethanol solution and soak them for 12 hours, then carry out heat reflux extraction for 3 times, each time for 2.5 hours, the extracts are combined and filtered, and the filtrate Dry at low temperature (50°C) until there is no alcohol smell to obtain the total water chestnut extract;

[0063] (2) Suspend the total water chestnut extract in water (V / V=1:1), knead and dissolve (i.e. fully dissolve), let stand for 20h, filter to obtain the supernatant; Ester, n-butanol are extracted, and the solvent is evaporated to dryness to obtain cyclohexane extract, ethyl acetate extract and n-butanol extract respectively;

[0064] (3) Dissolve the ethyl acetate extract prepared in step (2) with petroleum ether, and carry out silica gel column chromatography (wet column, 100-200 mesh); use petroleum ether-ethyl acetate elution system according to petroleum ether The volume ratio of ether-ethyl acetate is...

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Abstract

The invention belongs to the field of natural pharmaceutical chemistry and particularly relates to naphthoquinone compounds, preparation thereof and an application to hypoglycemic product preparation.The chemical structural formula of the naphthoquinone compounds is shown as formula I in the description. Preparation comprises the following steps: Eleocharis dulcis is ground and then subjected toheat reflux extraction, and total Eleocharis dulcis extract is obtained; extraction is performed with cyclohexane and ethyl acetate sequentially, an extract is separated and purified by a silica gel chromatographic column, a Sephadex LH-20 gel column, a Toyopearl HW-40 column and a semi-preparative liquid phase C18 column, and the naphthoquinone compounds are obtained. The naphthoquinone compoundsare novel in structure and have better hypoglycemic activity which is higher than that of positive control acarbose, and an important material basis is laid for development of hypoglycemic drugs withremarkable curative effect and few toxic and side effects from medicinal and edible plants in future.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a naphthoquinone compound and its preparation and application in the preparation of hypoglycemic products. Background technique [0002] Water chestnut (Heleocharis dulcis), commonly known as horseshoe and ground chestnut, is a perennial shallow water herb of Cyperaceae. As a kind of food with both medicine and food, it has tender meat, juicy and sweet taste, crisp and refreshing. It has the reputation of "Underground Sydney" since ancient times and is deeply loved by people. Every 100g of fresh water chestnuts contains 68g of water, 21.8g of carbohydrates, 1.5g of protein, 0.1g of fat, 0.5g of crude fiber, 5mg of calcium, 68mg of phosphorus, 0.5mg of iron, 0.01mg of carotene, and vitamin BB 1 0.04mg, vitamin B 2 0.02mg, Vitamin C 3mg, Niacin 0.4mg, etc. "Compendium of Materia Medica" records that water chestnuts can reduce fire, nourish the lungs and co...

Claims

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Application Information

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IPC IPC(8): C07D493/04A61P3/10A23L33/105
CPCA23L33/105A23V2002/00A61P3/10C07D493/04A23V2200/328A23V2250/21
Inventor 马勤阁魏荣锐
Owner NANYANG NORMAL UNIV
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