Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for separating and purifying Haematococcus pluvialis species

A technology for the separation and purification of Haematococcus pluvialis, which is applied in the field of purification of microalgae species, can solve the problems of difficult cultivation of single algae cells, influence on algae growth, induction of mutations, etc., and achieve stable properties, enhanced resistance, and low cost Effect

Active Publication Date: 2022-06-07
NINGBO FUTIAN BIOTECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently reported methods for purifying algae species mainly include picking algae cells under a microscope and adding antibiotics to the algae liquid to eliminate bacteria. The former is complicated to operate and has high technical requirements for operators, requiring certain experience in algae classification. Large and difficult to survive; the latter directly adds antibiotics to the algae liquid, although it can effectively inhibit the bacteria in the algae liquid, but the inability to completely eliminate bacteria will also affect the growth of algae and even induce mutations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Natural water samples. In the field sampling, Haematococcus pluvialis cells are the dominant species in a certain water area, and 500 mL of samples were sampled from the water area.

[0035] (1) Return to the laboratory, transfer the water sample to sterilized sterile water, and add the following nutrients: magnesium sulfate 0.02g / L, calcium chloride 0.01g / L, sodium citrate 0.1g / L , VB 1 10μg / L, VB 120.1μg / L, ferric chloride 0.588mg / L, manganese chloride 0.108mg / L, zinc sulfate 0.078mg / L, cobalt chloride 0.012mg / L, sodium molybdate 0.0075mg / L; using a light intensity of 5000lx, 24h / d light, pass through with CO 2 1% mixed gas (0.2vvm) was used for cultivation, the cultivation temperature was 23±2°C, and the cultivation time was 24h; after the cultivation, let stand for 1h, carefully remove the supernatant, and collect about 10mL of the algal liquid at the bottom of the cultivation container.

[0036] (2) Transfer the collected algal liquid into sterilized 15‰ sodium...

Embodiment 2

[0044] Natural water samples. Sampling in the field, there are Haematococcus pluvialis cells in a certain water area, Haematococcus pluvialis cells, some green algae and diatoms are all dominant algae strains, and 500mL is sampled from this water area. (1) Return to the laboratory, transfer the water sample to sterilized sterile water, and add the following nutrients: magnesium sulfate 0.02g / L, calcium chloride 0.01g / L, sodium citrate 0.1g / L , VB 1 10μg / L, VB 12 0.1μg / L, ferric chloride 0.588mg / L, manganese chloride 0.108mg / L, zinc sulfate 0.078mg / L, cobalt chloride 0.012mg / L, sodium molybdate 0.0075mg / L, using a light intensity of 5000lx, 24h / d light, pass through with CO 2 1% mixed gas (0.2vvm) was cultured, the culture temperature was 23±2°C, and the culture time was 48h; after the culture, let stand for 30min, carefully remove the supernatant, and collect about 10mL of algal fluid at the bottom of the culture vessel.

[0045] (2) Transfer the collected algal liquid int...

Embodiment 3

[0053] Natural water samples. When sampling in the field, it was found that there are Haematococcus pluvialis cells in a certain water area, but the dominant algae species are other green algae and diatoms. 500mL was sampled from the waters.

[0054] (1) Return to the laboratory, transfer the water sample to sterilized sterile water, and add the following nutrients: magnesium sulfate 0.02g / L, calcium chloride 0.01g / L, sodium citrate 0.1g / L , VB 1 10μg / L, VB 12 0.1μg / L, ferric chloride 0.588mg / L, manganese chloride 0.108mg / L, zinc sulfate 0.078mg / L, cobalt chloride 0.012mg / L, sodium molybdate 0.0075mg / L; using a light intensity of 5000lx, 24h / d light, pass through with CO 2 1% mixed gas (0.2vvm) was cultivated, the cultivation temperature was 23 ± 2 °C, and the cultivation time was 72h; after the cultivation, let stand for 15min, carefully remove the supernatant, and collect about 10mL of the algal liquid at the bottom of the cultivation container.

[0055] (2) Transfer th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
densityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for separating and purifying Haematococcus pluvialis algae species, which comprises selecting a water sample containing Haematococcus pluvialls cells or a single species of Haematococcus pluvialls algae liquid containing miscellaneous bacteria, adding suitable The inorganic medium for the growth of Haematococcus, after the induction culture treatment, let it stand still, and collect the cells at the bottom of the culture container; redissolve the collected algae cells in the prepared sterile sodium chloride solution, after standing still, remove the upper Collect the bottom cells in the supernatant; dissolve the bottom cells in the medium containing antibiotics for a certain period of time, let it stand, and settle; remove the supernatant, redissolve in sterile medium, and dilute to a certain concentration; dilute the diluted The solution is inserted into the culture well plate for cultivation; the purified algae strains are selected through microscopic examination and fungal detection. Through the method provided by the invention, algal species with higher purity can be obtained in a short time, the growth period is shortened, and the success probability of large-scale growth is improved.

Description

technical field [0001] The invention relates to the technical field of purification of microalgae algal species, in particular to a method for separating and purifying Haematococcus pluvialis algal species. Background technique [0002] Haematococcus pluvialis belongs to Chlorophyta, Chlorophyta, Volvox, Haematococcus, and is a kind of freshwater diflagellate unicellular green algae. Four typical cell forms appear throughout the life cycle: small worms, large flagellated worms, non-motile colloids, and large red cells with hard cell walls—Haematocysts. In a clean environment with sufficient nutrients, large parasites predominate; once the environment deteriorates, they transform into gelatinous bodies and later into erythrocysts. After the formation of red cysts, the cell density increases sharply (our study found that its density can be as high as 1.04-1.08g / cm3, while the remaining cell density is usually not higher than 1.02g / cm3), and the resistance to external adverse ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/02C12N1/12
Inventor 王兆伟宋佳巍王闪
Owner NINGBO FUTIAN BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com