Active nano-particles for degrading organophosphorus, as well as preparation method and applications of active nano-particles
A technology for degrading activity and nanoparticles, applied in biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, etc., can solve the problems of cumbersome operation and high cost, and achieve high recycling rate, low production cost, Good effect of repeated use
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0037] Example 1
[0038] 1.1Extraction of Cupriavidus necator genomic DNA
[0039] (1) Cultivate 5mL of actinomycete culture, take 1mL of culture and centrifuge at 12000rpm for 2min;
[0040] (2) Add 567 μL of TE buffer to the precipitate and resuspend it by repeated pipetting. Add 30 μL of 20 mg / mL lysozyme, water bath at 37 °C for 1 h;
[0041] (3) Add 300 μL of 10% SDS and 30 μL of 20 mg / mL proteinase K, mix well, and water bath at 37°C for 1 h;
[0042] (4) Add 100 μL 5M NaCl, mix well, then add 80 μL CTAB / NaCl solution, mix well, and take a 65-degree water bath for 10 minutes;
[0043] (5) adding equal volume of phenol / chloroform respectively, and extracting the chloroform solution once;
[0044] (6) Add 0.6 times the volume of isopropanol, mix gently, centrifuge at 12,000 rpm for 5 min, discard the supernatant, wash twice with 70% ethanol, and dry in an ultra-clean typhoon;
[0045] (7) Add 50 μL of TE buffer and dissolve the DNA overnight at 4°C.
[0046] 1.2 Amp...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap