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A method for joint fermentation of food waste and waste oil biodiesel by-product crude glycerol to produce lactic acid

A technology of food waste and biodiesel, applied in the direction of fermentation, etc., can solve the problems of food consumption, high cost, and aggravation of food and feed resources, and achieve the effects of improved utilization rate, low cost, good economic benefits and environmental benefits

Active Publication Date: 2021-08-03
汕头市利利新能源科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, 90% of lactic acid at home and abroad is fermented by microorganisms, mainly concentrated in the production of lactic acid from the hydrolyzed sugar of food crops (such as barley, starch, corn, potatoes, etc.), which is costly and large-scale industrial production of lactic acid as Polylactic acid synthetic raw materials will consume a large amount of food, which will aggravate the tension of food and feed resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The food waste is sorted to select inorganic components, and the remaining food waste is crushed and homogenized for use. The waste oil biodiesel crude glycerin is absorbed by bagasse (in a sugar factory, crushed into 40 meshes) at 50°C to 55°C for 24 hours, and then used after press filtration. The mass ratio of waste oil biodiesel crude glycerin to biomass is 0.05. Each gram of mixed hydrolytic enzyme liquid includes: glucoamylase 100U, adding amount 0.002g, (specification 50000U / g); cellulase 100U, adding amount 0.002g, (specification 50000U / g); amylase 100U, adding amount 0.02g , (specification 5000U / g); lipase 50U, adding amount 0.005g, (specification 10000U / g); protease 50U, adding amount 0.001g, (specification 50000U / g); pectinase 10U, adding amount 0.001g, ( Specification 10000U / g); the balance is sterile water.

[0026] The formula of MRS medium is: glucose 20g, peptone 10g, yeast powder 5g, beef extract 10g, sodium acetate 5g, dipotassium hydrogen phosphate 2...

Embodiment 2

[0048] The food waste is sorted to select inorganic components, and the remaining food waste is crushed and homogenized for use. Waste oil biodiesel crude glycerol is absorbed by peanut shells (crushed into 40 meshes) at 55°C to 60°C for 24 hours, and then used after press filtration. The mass ratio of waste oil biodiesel crude glycerol to biomass is 0.08. Each gram of mixed hydrolytic enzyme liquid includes: glucoamylase 130U, adding amount 0.0026g, (specification 50000U / g); cellulase 75U, adding amount 0.0015g, (specification 50000U / g); amylase 130U, adding amount 0.026g , (specification 5000U / g); lipase 75U, adding amount 0.0075g, (specification 10000U / g); protease 30U, adding amount 0.0006g, (specification 50000U / g); pectinase 30U, adding amount 0.003g, ( Specification 10000U / g); the balance is sterile water.

[0049] The formula of MRS medium is: glucose 20g, peptone 10g, yeast powder 5g, beef extract 10g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, diammonium ...

Embodiment 3

[0056] The food waste is sorted to select inorganic components, and the remaining food waste is crushed and homogenized for use. The waste oil biodiesel crude glycerol is absorbed by the mixture of corncob and straw at 60℃~65℃ for 24 hours, and then it is ready to be used after pressing. The mass ratio of waste oil biodiesel crude glycerin to biomass is 0.1, and the mass ratio of corncob to straw 1:1. Each gram of mixed hydrolytic enzyme solution includes: 160U of glucoamylase, 100U of cellulase, 160U of amylase, 100U of lipase, 50U of protease, 50U of pectinase, and the balance is sterile water.

[0057] The formula of MRS medium is: glucose 20g, peptone 10g, yeast powder 5g, beef extract 10g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, diammonium hydrogen citrate 2g, Tween 80 1mL, MgSO 4 .7H 2 O 0.58g, MnSO 4 .H 2 Add 0.25g of O into water, then distill the volume to 1000mL with distilled water, adjust the pH to 6.2, and sterilize at 121°C for 20min.

[0058] ...

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Abstract

The invention provides a method for producing lactic acid by joint fermentation of kitchen waste and waste oil biodiesel by-product crude glycerol. The method comprises the following steps: adding mixed hydrolase to the food waste culture medium for hydrolysis, inoculating the seed liquid into the hydrolyzed food waste culture medium, and adding the crude glycerol of waste oil and biodiesel which has been preliminarily treated by biomass, Anaerobic or micro-aerobic fermentation, at the same time, the crude glycerol of waste oil and biodiesel that has been preliminarily treated by biomass is fed in, and the concentration of crude glycerol in the fermentation system is controlled to be 10-80g / L, pH is 5.0-8.0, and glycerol and lactic acid are measured during the fermentation process. The content of the fermented liquid obtained after fermentation was filtered to obtain the lactic acid liquid. The invention obtains a new way of lactic acid fermentation with lower cost and wider source of raw materials, and has good economic and environmental benefits, which broadens the selection range of raw materials for lactic acid fermentation, and is wastes such as kitchen waste and waste glycerin. resource utilization provides a new way.

Description

Technical field: [0001] The invention belongs to the technical field of lactic acid fermentation, and in particular relates to a method for producing lactic acid by joint fermentation of kitchen waste and crude glycerol, a by-product of waste oil biodiesel. Background technique: [0002] Lactic acid, namely 2-hydroxypropionic acid, has become one of the three most recognized organic acids in the world since it was discovered by Swedish chemist Scheele from yogurt in 1780. As important chemical raw materials, lactic acid and its derivatives have been widely used in food, pharmaceutical, textile, leather and other chemical industries. In recent years, due to the increasingly serious "white pollution", biodegradable materials have become more and more popular. Polylactic acid products are one of the biodegradable plastics that have been studied more and have better performance. Its products have broad application prospects in agriculture, life, clothing and medical industries....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56
CPCC12P7/56C12P2201/00
Inventor 李惠文苗长林杨玲梅罗文李志兵王治元付俊鹰吕鹏梅
Owner 汕头市利利新能源科技有限公司
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