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Bacteroides fragilis capable of relieving endotoxin infection as well as application thereof

A technology of Bacteroides fragilis and microbial strains, applied in the field of microbiology, can solve the problems of unconcerned strains, change intestinal permeability, etc., and achieve the effects of low immunogenicity, increased concentration, and wide application prospects

Active Publication Date: 2018-10-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no patent document reporting the relieving effect of B. fragilis on endotoxin infection, especially the way in which this type of strain changes intestinal permeability.

Method used

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  • Bacteroides fragilis capable of relieving endotoxin infection as well as application thereof
  • Bacteroides fragilis capable of relieving endotoxin infection as well as application thereof
  • Bacteroides fragilis capable of relieving endotoxin infection as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Cultivation and preservation of Bacteroides fragilis CCFM1020

[0048] 1. Preparation of culture medium: Prepare brain-heart infusion BHI medium (such as the product of Qingdao Haibo Biotechnology Co., Ltd.), dissolve it in distilled water, and add cysteine ​​hydrochloride 1g / L, hemin 0.01g / L, vitamin K 10.002g / L, mix evenly, then adjust the pH to 7.0, and sterilize at 115-121°C for 15-20min to obtain the culture medium.

[0049] 2. Cultivation method: Bacteroides fragilis CCFM1020 strains are inoculated according to the inoculation amount of 2-4% based on the weight of the above-mentioned culture medium, and anaerobically cultured at 37° C. for 12-18 hours, reaching a stable period.

[0050] 3. Preparation of protective agent: Weigh 1 g / L of cysteine ​​hydrochloride and 200 g / L of glycerin, dissolve them in distilled water evenly, and sterilize at 115-121° C. for 15-20 minutes to obtain the protective agent.

[0051] 4. Preservation method: After cleaning t...

Embodiment 2

[0052] Example 2: Tolerance dose experiment of Bacteroides fragilis CCFM1020 administered to mice

[0053] Get 10 healthy female C57 mice of the age of 6-8 weeks, and the Bacteroides fragilis CCFM1020 cryopreservation agent (10 10 CFU / ml), observed for 5 days, and recorded the body weight and death status of the mice.

[0054] The results are shown in Table 1, and the feeding concentration was 10 9 CFU of Bacteroides fragilis CCFM1020 had no significant effect on the mice, with weight gain and no death. The appearance of the mice had no obvious pathological symptoms.

[0055] Table 1: Feeding 10 9 Effect of CFU of Bacteroides fragilis CCFM1020 on body weight of mice

[0056]

Embodiment 3

[0057] Example 3: Immune tolerance experiment of mice fed with Bacteroides fragilis CCFM1020

[0058] Twenty healthy female C57 mice aged 6-8 weeks were randomly divided into two groups: the negative control group and the B. fragilis CCFM1020 intervention group, with 10 mice in each group. The mice in the Bacteroides fragilis CCFM1020 intervention group were given 0.1ml of the above-mentioned Bacteroides fragilis CCFM1020 cryopreservation agent once every 24 hours (10 10 CFU / ml), the mice in the control group were fed with 0.1ml of the protective agent of the above-mentioned cryopreservation agent every 24 hours, and all the mice were sacrificed after continuous feeding for 5 days. Technology Co., Ltd.) after lysing and homogenizing, the concentration of immune factors was determined using an ELISA kit (product of Nanjing Senbega Biotechnology Co., Ltd.).

[0059] The experimental results are attached figure 1 As shown, the contents of immune factors TNF-α, IL-6, IL-10 and C...

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Abstract

The invention discloses bacteroides fragilis capable of relieving endotoxin infection as well as application thereof, and belongs to the technical field of microorganisms. The bacteroides fragilis CCFM1020 has low immunogenicity, and can reduce the content of proinflammatory factors in blood of a host infected with endotoxin, increase the concentration of anti-inflammatory factors, up-regulate thenumber of Foxp3+ regulatory T cells and stabilize composition of intestinal flora. The bacteroides fragilis CCFM1020 is a medicinal composition for relieving endotoxin infection and has a wide application prospect.

Description

technical field [0001] The invention relates to a Bacteroides fragilis for alleviating endotoxin infection and application thereof, belonging to the technical field of microorganisms. Background technique [0002] Endotoxin, also known as lipopolysaccharide, is an important component of the cell wall of Gram-negative bacteria. Endotoxin has a stable structure and is mainly composed of three parts: polysaccharide O antigen, core polysaccharide and lipid A (lipid A). Lipid A is a lipidated glucosamine disaccharide that is pyrogenic and a toxic component of Gram-negative bacterial endotoxins. Under normal circumstances, endotoxins are only released when the bacteria die and dissolve or the bacterial cells are destroyed by artificial methods, and the structure of endotoxins is stable. biological activity. [0003] The intestinal lumen of a normal body contains a large number of intestinal bacteria, and the intestinal mucosa can selectively absorb nutrients and prevent the ent...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A61K35/741A61P39/02A61P1/00C12R1/01
CPCA61P1/00A61P39/02C12N1/04C12N1/20A61K2035/115C12R2001/01C12N1/205A61P31/04A61K35/74A61K9/19A61K9/2009A61K9/2018A61K9/2054A61K9/2059A61K9/48A61K9/16A61K35/741
Inventor 翟齐啸陈卫谭惠子陆文伟田丰伟赵建新张灏
Owner JIANGNAN UNIV
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