Bacteroides fragilis capable of relieving endotoxin infection as well as application thereof

A technology of Bacteroides fragilis and microbial strains, applied in the field of microbiology, can solve the problems of unconcerned strains, change intestinal permeability, etc., and achieve the effects of low immunogenicity, increased concentration, and wide application prospects

Active Publication Date: 2018-10-02
JIANGNAN UNIV
View PDF9 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no patent document reporting the relieving effect of B. fragilis on en

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacteroides fragilis capable of relieving endotoxin infection as well as application thereof
  • Bacteroides fragilis capable of relieving endotoxin infection as well as application thereof
  • Bacteroides fragilis capable of relieving endotoxin infection as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1: Cultivation and storage of Bacteroides fragilis CCFM1020

[0048] 1. Preparation of culture medium: Prepare brain heart infusion BHI culture medium (such as the product of Qingdao Haibo Biotechnology Co., Ltd.), dissolve it in distilled water, and add 1g / L of cysteine ​​hydrochloride and hemin 0.01g / L, vitamin K10.002g / L, mix well, then adjust the pH to 7.0, and sterilize at 115-121°C for 15-20 minutes to obtain the medium.

[0049] 2. Cultivation method: Bacteroides fragilis CCFM1020 strain is inoculated according to the inoculation amount of 2-4% based on the weight of the above-mentioned medium, and cultivated anaerobic at 37°C for 12-18h, which can reach a stable period.

[0050] 3. Preparation of the protective agent: Weigh 1 g / L of cysteine ​​hydrochloride and 200 g / L of glycerin, uniformly dissolve them in distilled water, and sterilize at 115-121°C for 15-20 minutes to obtain the protective agent.

[0051] 4. Preservation method: Wash the stable bacterial liq...

Example Embodiment

[0052] Example 2: Tolerable dose experiment of Bacteroides fragilis CCFM1020 gavage mice

[0053] Take 10 healthy female C57 mice aged 6-8 weeks and gavage 0.1ml of the Bacteroides fragilis CCFM1020 cryopreservant prepared in Example 1 every 24 hours (10 10 CFU / ml), observe for 5 days, record the weight and death of mice.

[0054] The results are shown in Table 1, the feeding concentration is 10 9 The CFU Bacteroides fragilis CCFM1020 did not cause significant effects on mice, weight increased, and no deaths occurred. The mice have no obvious pathological symptoms.

[0055] Table 1: Feeding 10 9 Effect of CFU Bacteroides fragilis CCFM1020 on the body weight of mice

[0056]

Example Embodiment

[0057] Example 3: Immune tolerance experiment of Bacteroides fragilis CCFM1020 gavage mice

[0058] Twenty healthy female C57 mice aged 6-8 weeks were randomly divided into two groups: the negative control group and the Bacteroides fragilis CCFM1020 intervention group, each containing 10 mice. Bacteroides fragilis CCFM1020 intervention group mice were given 0.1ml of the above-mentioned Bacteroides fragilis CCFM1020 cryopreservant (10 10 CFU / ml), the mice in the control group were given 0.1ml of the protective agent of the cryopreservative once every 24 hours. All mice were sacrificed after 5 days of intragastric administration. The blood and colon tissue were taken from the RIPA lysate (Biyuntian Bio After lysing the homogenate, the ELISA kit (product of Nanjing Senbega Biotechnology Co., Ltd.) was used to determine the concentration of immune factors.

[0059] The experimental results are attached figure 1 As shown, the levels of immune factors TNF-α, IL-6, IL-10, and CXCL-2 in th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses bacteroides fragilis capable of relieving endotoxin infection as well as application thereof, and belongs to the technical field of microorganisms. The bacteroides fragilis CCFM1020 has low immunogenicity, and can reduce the content of proinflammatory factors in blood of a host infected with endotoxin, increase the concentration of anti-inflammatory factors, up-regulate thenumber of Foxp3+ regulatory T cells and stabilize composition of intestinal flora. The bacteroides fragilis CCFM1020 is a medicinal composition for relieving endotoxin infection and has a wide application prospect.

Description

technical field [0001] The invention relates to a Bacteroides fragilis for alleviating endotoxin infection and application thereof, belonging to the technical field of microorganisms. Background technique [0002] Endotoxin, also known as lipopolysaccharide, is an important component of the cell wall of Gram-negative bacteria. Endotoxin has a stable structure and is mainly composed of three parts: polysaccharide O antigen, core polysaccharide and lipid A (lipid A). Lipid A is a lipidated glucosamine disaccharide that is pyrogenic and a toxic component of Gram-negative bacterial endotoxins. Under normal circumstances, endotoxins are only released when the bacteria die and dissolve or the bacterial cells are destroyed by artificial methods, and the structure of endotoxins is stable. biological activity. [0003] The intestinal lumen of a normal body contains a large number of intestinal bacteria, and the intestinal mucosa can selectively absorb nutrients and prevent the ent...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/04A61K35/741A61P39/02A61P1/00C12R1/01
CPCA61P1/00A61P39/02C12N1/04C12N1/20A61K2035/115C12R2001/01C12N1/205A61P31/04A61K35/74A61K9/19A61K9/2009A61K9/2018A61K9/2054A61K9/2059A61K9/48A61K9/16A61K35/741
Inventor 翟齐啸陈卫谭惠子陆文伟田丰伟赵建新张灏
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap