Yeast strain and application thereof

A technology of yeast strain and Torula sporogenes, applied in the field of microorganisms, can solve the problem of less non-Saccharomyces cerevisiae species, etc., and achieve the effects of strong alcohol tolerance, strong fermentation ability, and increasing the glycerin content of fruit wine

Inactive Publication Date: 2018-10-09
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some studies have shown that non-Saccharomyces cerevisiae can improve the quality of wine, but at present, there are very few studies o...

Method used

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  • Yeast strain and application thereof
  • Yeast strain and application thereof
  • Yeast strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 The physiological characteristics of Torulaspora delbrueckii (Torulaspora delbrueckii) R12

[0039] 1.1 Source of strain

[0040] Torulaspora delbrueckii R12, R85, Gansu Qilian winery. Strains R12 and R85 were isolated from the natural fermentation process of wine in Qilian winery in Gansu Province, and belonged to native yeasts in my country. Wherein, the D1-D2 region of strain R8526s has the nucleotide sequence shown in SEQ ID: 1, and the D1-D2 region of strain R1226s has the nucleotide sequence shown in SEQ ID: 2. In addition, the growth morphology of strains R85 and R12 on WLN medium is shown in figure 1 Shown, wherein, A is the growth form figure of bacterial strain R85, and B is the growth form figure of bacterial strain R12.

[0041] 1.2 Experimental content and methods

[0042] 1.2.1 Drawing of growth curve

[0043] The strains Torulaspora delbrueckii R12 and R85 stored at -20°C were activated in YPD liquid medium at 28°C and 150r / min for 24h. Us...

Embodiment 2

[0075] Example 2 Research on Fermentation Characteristics of Torulaspora delbrueckii R12 and Its Application in Fermentation

[0076] The fermentation characteristics of the above-mentioned Torula delicae R85 and R12 were studied using Triple M simulated juice, and the formula of Triple M simulated juice was:

[0077]Ergo stock: 12.5mL Tween80, 37.5mL 95% ethanol, 0.125g ergosterol, solution A: add 100g glucose, 100g fructose to 375mL deionized water, 4mL ergo stock, add deionized water after dissolving to make up to 500mL; solution B: Add 6g L(+) tartaric acid, 3g L(-) malic acid, 0.5g citric acid in 250mL deionized water; solution C: add 1.7g YNB, 2g acid hydrolyzed casein, 6mg inositol, 0.2g no Calcium chloride hydrate, L-arginine 0.8g, L-proline 1g / L, DL-tryptophan 0.1g / L, ammonium phosphate 1g. After A, B, and C are mixed, adjust the pH to 3.25 with 4mol / L potassium hydroxide, filter through a 0.22μm filter membrane, and prepare and use immediately.

[0078] Treatment 1...

Embodiment 3

[0103] Example 3 Effect of mixed fermentation of T.delbrueckii and S.cerevisiae on the quality of sweet white wine

[0104] The grape juice was mixed and fermented with Torula dell'Kais R12 and Saccharomyces cerevisiae NX11424, in which Saccharomyces cerevisiae NX11424 was stored in the microbial strain preservation room of Northwest A&F University. Grape variety: Chardonnay; picking time: September 2015 ;Physical and chemical properties of grape juice: total sugar 239g / L, total acid 6.6g / L, pH 3.7.

[0105] Fermented by Tianbai's small container brewing method, the total inoculation amount for each fermentation is 5×10 5 cells / mL (hemocytometer method), static fermentation, fermentation temperature 18 ℃, set up two parallel. During the fermentation process, the fermentation process is indirectly monitored by measuring the specific gravity, and the basic physical and chemical indicators and the content of volatile substances are measured after the fermentation is completed. ...

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Abstract

The invention provides a yeast strain kept under CGMCC NO: 15430 and named as Torulaspora delbrueckii. Experimental results show that the yeast strain has good alcohol tolerance, SO2 tolerance and high sugar tolerance, has a fermenting function in a fermenting broth having a sugar concentration of 200-400 g/L, and features low production of volatile acids and high production of glycerol; in brewedsweet white wine and ice wine, the yeast strain can be mixed with Saccharomyces cerevisiae and fermented to reduce the yield of fruit wine volatile acids, improve the content of fruit wine glycerol,and impart more flower aroma to fruit wines. The yeast strain provided herein has high fermenting capacity and good fermenting performance, and has important value in the application to improve fruitwine quality.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a yeast strain and application thereof. Background technique [0002] Saccharomyces cerevisiae has always been the main strain of fruit wine fermentation, and a large part of the fermentation strains of the fruit wine we produce use Saccharomyces cerevisiae. In recent years, some researchers have found that the aroma substances produced by non-Saccharomyces cerevisiae account for 90% of the total aroma components of wine. Among them, Torulaspora delbrueckii is a non-Saccharomyces cerevisiae related to wine and belongs to true yeast (the anamorph of Candida colliculosa). Its cells are small and nearly round (6.5 μm × 5.5 μm). The colonies on WLN medium are creamy with light green, spherical protrusions, and the surface is smooth and opaque. T.delbrueckii has a 9-11Mb genome distributed on 8 chromosomes. It is generally believed that the fermentative capacity ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12G3/02C12R1/645
CPCC12G3/02C12N1/145C12R2001/645
Inventor 刘延琳叶冬青宋育阳秦义刘芳利
Owner NORTHWEST A & F UNIV
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