Quick extraction method for egg yolk antibody, prepared anti-burn, anti-scald and anti-infection product and application of egg yolk antibody

Abstract

The invention discloses a quick extraction technology for an egg yolk antibody, a prepared anti-burn, anti-scald and anti-infection product and application of the egg yolk antibody. The technology comprises the steps that whole-cell vaccines of pseudomonas aeruginosa, escherichia coli and staphylococcus aureus are used for conducting immunization on laying hens; eggs are automatically and mechanically separated to obtain egg yolk, a citric acid-sodium hydrogen phosphate buffer solution is diluted, and the pH value of an egg yolk solution is stabilized; dry ice is added for quickly lowering thesystem temperature; continuous high-speed low-temperature centrifugation is conducted, fat particles are removal, and an egg yolk antibody supernatant is obtained; the egg yolk antibody supernatant passes 2-6micron and 0.3-0.8micron double-layer filter membranes and a hollow fiber ultrafiltration membrane with cut-off molecular weight of 300 kD in sequence to remove miscellaneous protein and finally passes through a hollow fiber ultrafiltration membrane with cut-off molecular weight of 100 kD to obtain a concentrated egg yolk antibody solution; ingredients are added to prepare a skin externalspray agent. The technology has the advantages that the automation degree is high, the energy consumption is low, the yield is high, the environment is protected, and large-scale industrialized production can be achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, especially the biotechnology of obtaining antibodies through immunization and breeding technology and preparing them into medicines or health care products, and particularly relates to a rapid extraction method of egg yolk antibodies, prepared anti-burn infection products and applications thereof. Background technique [0002] Extensive burns and scalds have a fatal risk, which is closely related to post-burn infection. After burns, the natural skin barrier is destroyed, and the necrotic skin tissue becomes a nutrient substance for bacteria. In addition, the insufficient supply of skin blood vessels at the burn and scald and the immunosuppression caused by systemic disorders aggravate the occurrence of infection. The pathogens of burn and scald infection include bacteria, fungi and viruses, among which the most common bacteria are Pseudomonas aeruginosa (high drug resistance), Staphylococcus ...

AI Technical Summary

Problems solved by technology

For example: collecting egg yolk by hand-beating eggs is inefficient and not suitable for large-scale use, and it is easy to introduce pollution sources, increasing the risk of contamination of egg yolk antibodies; using repeated freezing and thawing 1-3 times, if used The production operation is time-consuming and laborious; use organic solvents such as caprylic acid or chloroform to extract IgY, the application of organic solvents affects the activity of IgY, and is toxic, pollutes the environment, and is inconvenient to operate; adopts ammonium sulfate salting out precipitation method, there are centrifugation to collect precipitation and Time-consuming operations such as reconstitution and precipitation; the water dilution extraction method is used to extract IgY, although it is suitable for large-scale production and protection of IgY activity, but the extraction time is prolonged. Lowered to 0-8°C, the production process is time-consuming and energy-consuming in actual production, and the efficiency of large-scale industrial production is low

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