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Preparation method of modified polysaccharide hydrogel and prepared modified polysaccharide hydrogel

A hydrogel and polysaccharide technology, applied in the field of hydrogel preparation, can solve the problems of poor biocompatibility and harsh synthesis conditions, achieve high equilibrium water content, simple reaction conditions, and improve biocompatibility

Active Publication Date: 2018-10-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the shortcomings of many synthetic materials in the prior art, such as poor biocompatibility and harsh synthesis conditions, the present invention provides a method for preparing a modified polysaccharide hydrogel and the prepared modified polysaccharide hydrogel. The prepared hydrogel The glue has good biocompatibility, anti-protein adsorption performance, self-healing performance and high equilibrium moisture content, etc.

Method used

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  • Preparation method of modified polysaccharide hydrogel and prepared modified polysaccharide hydrogel
  • Preparation method of modified polysaccharide hydrogel and prepared modified polysaccharide hydrogel
  • Preparation method of modified polysaccharide hydrogel and prepared modified polysaccharide hydrogel

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Embodiment 1

[0037] The invention provides a kind of preparation method of modified polysaccharide hydrogel, comprising the following steps:

[0038] (1) Preparation of double bond modified polysaccharide:

[0039] Mix 0.5g of hyaluronic acid and water evenly in proportion to a concentration of 3wt%, under magnetic stirring, add 100μL of triethylamine, 0.47g of tetrabutylammonium bromide, 3.2mL of glycidyl methacrylate, and react for 24 hours , followed by dialysis in 0.1M sodium chloride and deionized water for 12 hours, and lyophilized to obtain double bond modified hyaluronic acid (HAGMA);

[0040] (2) Preparation of zwitterionic thiol polymer (CB-SH):

[0041] Dissolve 0.5 g of carboxybetaine methyl methacrylate (CBMA) and 40 mg of N,N'-bis(acryloyl) cystamine (BAC) in a mixed solvent of 4 ml of water and ethanol, then add 16 mg of ammonium persulfate, 20 μL N,N,N',N'-tetramethylethylenediamine, reacted for 24h, dialyzed the reaction product in deionized water for three days to remov...

Embodiment 2

[0047] The invention provides a kind of preparation method of modified polysaccharide hydrogel, comprising the following steps:

[0048] (1) The preparation of the double bond modified polysaccharide is the same as in Example 1 step (1);

[0049] (2) The preparation of the zwitterionic mercapto polymer (CB-SH) is the same as in Example 1 step (2);

[0050] (3) Preparation of "click" chemical hydrogel:

[0051] Dissolve 50mg HAGMA in 1ml PBS (PH=7.2) buffer solution, stir well, and set aside; Dissolve 0.1g CB-SH in 1ml PBS (PH=7.2) buffer solution, stir well, set aside; Take a certain amount of two kinds of Mix the mother liquor evenly according to the ratio of double bond-mercapto group of 2:1, put it in a constant temperature water bath at 37°C, and gel can be formed spontaneously within 1 hour.

Embodiment 3

[0053] The invention provides a kind of preparation method of modified polysaccharide hydrogel, comprising the following steps:

[0054] (1) The preparation of the double bond modified polysaccharide is the same as in Example 1 step (1);

[0055] (2) The preparation of the zwitterionic mercapto polymer (CB-SH) is the same as in Example 1 step (2);

[0056] (3) Preparation of "click" chemical hydrogel:

[0057] Dissolve 50mg HAGMA in 1ml PBS (PH=7.2) buffer solution, stir well, and set aside; Dissolve 0.1g CB-SH in 1ml PBS (PH=7.2) buffer solution, stir well, set aside. Take a certain amount of two kinds of mother liquors, mix them evenly according to the ratio of double bond-mercapto group of 1:2, put them in a constant temperature water bath at 37°C, and gel can be formed spontaneously within 1 hour.

[0058] In order to further illustrate the performance of the hydrogel prepared by the present invention, due to limited space, only Example 1 is used as an example to constru...

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Abstract

The invention discloses a preparation method of a modified polysaccharide hydrogel and a prepared modified polysaccharide hydrogel, and belongs to the field of gel preparation. The preparation methodcomprises following steps: (1) preparing double bond modified polysaccharide; (2) preparing zwitter-ion thiol polymer (CB-SH); and (3) preparing click-chemistry hydrogel. The provided hydrogel can beformed under physiological conditions, the reaction conditions are simple and mild; through the cooperation between hyaluronic acid and zwitter-ion polymer, the biocompatibility of the hydrogel is improved; and the hydrogel has a high equilibrium water content, an anti-protein property, and a self-healing performance.

Description

technical field [0001] The invention relates to the technical field of hydrogel preparation, in particular to a preparation method of a modified polysaccharide hydrogel and the prepared modified polysaccharide hydrogel. Background technique [0002] Hydrogel is a three-dimensional cross-linked polymer network, which is soft in nature, absorbs a large amount of water and is insoluble in water while maintaining its integrity. Due to their excellent swelling-deswelling properties and stimuli-response behavior, hydrogels have been widely used in soft contact lenses, chemical sensors, drug-controlled release materials, tissue engineering, wound dressings, and diagnostic technologies. [0003] All water-soluble or hydrophilic polymers can form hydrogels through certain chemical cross-linking or physical cross-linking. These polymers can be divided into two categories according to their sources: natural and synthetic. Natural hydrophilic polymers include polysaccharides (starch, c...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08B37/08C08F220/36C08F222/38C08L5/08C08L33/14
CPCC08B37/0072C08F220/36C08J3/075C08J2305/08C08J2433/14C08F220/365C08F222/385
Inventor 张亚彬刘淑艳张书香宗传永张炉青马佳晨翟丛丛曹影
Owner UNIV OF JINAN
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