A tumor-targeted nano-diagnostic agent, its preparation method and its use
A tumor targeting and nanotechnology, applied in the field of biomedical materials and nanomaterials, can solve the problems of lack of release specificity, exogenous stimulation, and cumbersome chemical synthesis process of nanodiagnostic agents.
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[0031] see Figure 1 to Figure 2 , a synthesis schematic diagram and a synthesis flow chart of a nano-therapeutic agent in a preferred embodiment of the present invention. The preparation method of the nano-medicine comprises the following steps:
[0032] Step S100, preparing solid silicon dioxide (SiO 2 ) nanoparticles.
[0033] Specifically, the organic solvent, emulsifier, and alkali source are mixed, and the silicon source is added dropwise to react at an appropriate temperature for a period of time. Then add a demulsifier, centrifuge and wash to obtain the solid SiO 2 nanoparticles.
[0034] The organic solvent, the emulsifier, the alkali source and the demulsifier are the preparation of SiO in this field 2 Commonly used raw materials for nanoparticles. The organic solvent is cyclohexane, the emulsifier is a commercially available product, the product model is CO-520, the alkali source is ammonia, and the silicon source is tetraethyl orthosilicate (TEOS). The emuls...
Embodiment 1
[0070] (1) Preparation of solid silica nanoparticles.
[0071] With 60mL cyclohexane as the oil phase, 3g CO@520 as the emulsifier, 420uL 30% ammonia water as the alkali source, and 600uLTEOS as the silicon source, add dropwise (the dropping rate is controlled at 0.003mL / min), and the reaction temperature is controlled at 20°C, stirred for 24h. Using 10 mL of anhydrous methanol as a demulsifier, centrifuge at 10,000 rpm to obtain silica nanoparticles, wash with alcohol solution and water, and redisperse in 6 mL of deionized water to obtain SiO 2 solution.
[0072] (2) Preparation of core-shell SiO 2 @MSN.
[0073] Add 2mL of CTAC and 200uL TEAH (10wt%) into 11mL of deionized water, stir well, then add 2mL of the SiO 2 solution. The temperature was raised to 80°C, and an appropriate amount of TEOS was added at a certain rate (the dropping rate was controlled at 0.001 mL / min), and the reaction was continued for 12 hours. After centrifugal washing, to obtain core-shell SiO ...
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