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Composite microbial inoculant for degrading shale gas-water based drilling cutting pollutants as well as preparation method and use method of composite microbial inoculant

A compound inoculant and microorganism technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, and the restoration of polluted soil, can solve the problems of land occupation, surrounding soil and water pollution, and environmental risks, etc. The effect of harmless treatment, beneficial to resource utilization, and reducing the risk of pollution

Inactive Publication Date: 2018-11-02
重庆市涪陵页岩气环保研发与技术服务中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main methods for the harmless treatment of water-based drilling cuttings: one is solidification and landfill, but it occupies a large area of ​​land and the pollutants have not been completely decomposed. Settlement, especially the erosion and immersion effect of long-term rainfall on the solidification pool, the leachate of the solidification body may seep out and cause certain pollution to the surrounding soil and water bodies
The second is the utilization of building materials, such as preparing unburned bricks, concrete, and road cushions with water-based drilling cuttings. However, the utilization of building materials only temporarily fixes and seals water-based drilling cuttings pollutants, and its potential environmental risks still exist

Method used

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  • Composite microbial inoculant for degrading shale gas-water based drilling cutting pollutants as well as preparation method and use method of composite microbial inoculant
  • Composite microbial inoculant for degrading shale gas-water based drilling cutting pollutants as well as preparation method and use method of composite microbial inoculant
  • Composite microbial inoculant for degrading shale gas-water based drilling cutting pollutants as well as preparation method and use method of composite microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Pseudomonas aeruginosa, Acinetobacter, Bacillus subtilis, and Micrococcaceae were respectively cultured on a test tube slant, activated by inoculating shake flasks, and cultured in seed tanks for the first generation, respectively. The first-generation bacterial suspension cultured in the seed tank was inserted into the fermenter liquid culture according to the inoculation amount of 5% of the fermenter medium volume. Yeast extract of 1.5% of water quality, sodium chloride of 0.1% of water quality, dipotassium hydrogen phosphate of 0.5% of water quality, in the first-generation bacterial suspension, Pseudomonas aeruginosa, Acinetobacter, Bacillus subtilis, Micrococci were inoculated at a mass ratio of 1:0.5:1:1, the culture temperature was 25°C, and the culture time was 24 hours. Microscopic examination, when the number of viable bacteria in the composite bacterial agent reached 10 8 cfu / ml, a liquid microbial composite bacterial agent for degrading shale gas water-based...

Embodiment 2

[0024] Pseudomonas aeruginosa, Acinetobacter, Bacillus subtilis, and Micrococcaceae were respectively cultured on a test tube slant, activated by inoculating shake flasks, and cultured in seed tanks for the first generation, respectively. The first-generation bacterial suspension cultured in the seed tank is inserted into the fermenter liquid culture according to the inoculum volume of 10% of the fermenter medium volume. Yeast paste with 2% water quality, sodium chloride with 0.15% water quality, and dipotassium hydrogen phosphate with 0.7% water quality are composed. Micrococci were inoculated at a mass ratio of 2:1:2:2, the culture temperature was 35°C, and the culture time was 72 hours. Microscopic examination showed that when the number of viable bacteria in the compound bacterial agent reached 10 12 cfu / ml, a liquid microbial composite bacterial agent for degrading shale gas water-based drilling cuttings pollutants was prepared.

[0025] Take 5 kg of shale gas water-base...

Embodiment 3

[0027] Pseudomonas aeruginosa, Acinetobacter, Bacillus subtilis, and Micrococcaceae were respectively cultured on a test tube slant, activated by inoculating shake flasks, and cultured in seed tanks for the first generation, respectively. The first-generation bacterial suspension cultured in the seed tank is inserted into the fermenter liquid culture according to the inoculum volume of 10% of the fermenter medium volume. Yeast paste with 2% water quality, sodium chloride with 0.15% water quality, and dipotassium hydrogen phosphate with 0.7% water quality are composed. Micrococci were inoculated at a mass ratio of 2:1:2:2, the culture temperature was 35°C, and the culture time was 72 hours. Microscopic examination showed that the number of viable bacteria in the composite bacterial agent reached 10 12cfu / ml. Add 30% of the mass of the fermented liquid microbial composite bacterial agent to the bran after high temperature sterilization (it can also be any one or several of cind...

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Abstract

The invention relates to a composite microbial inoculant for degrading shale gas-water based drilling cutting pollutants as well as a preparation method and a use method of the composite microbial inoculant. A microbe main body of the composite microbial inoculant is prepared from pseudomonas aeruginosa, acinetobacter, bacillus subtilis and micrococcus which are mixed according to the mass ratio of (1-2) to (0.5-1) to (1-2) to (1-2), wherein the content of living bacteria number of the composite inoculant is 108-1,012 cfu / ml. The composite microbial inoculant can perform effective degradationon the shale gas-water based drilling cutting pollutants.

Description

technical field [0001] The invention relates to a microbial composite bacterial agent, in particular to a microbial composite bacterial agent for degrading shale gas water-based drilling cuttings pollutants and a preparation and use method thereof. Background technique [0002] Shale gas water-based drilling cuttings is a kind of solid waste produced during shale gas drilling that mixes formation cuttings and water-based drilling fluid, and the production volume of a single well is about 800-1000 m 3 . Due to the adsorption of a small amount of water-based drilling fluid additives on the surface of water-based drilling cuttings, its composition is relatively complex and contains a certain amount of pollutants such as petroleum hydrocarbons, salt, and heavy metals. Therefore, the harmless treatment of water-based drilling cuttings has always been the focus of post-drilling treatment in shale gas development. At present, the main methods for the harmless treatment of water-b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/385C12R1/01C12R1/125C02F103/10
CPCB09C1/10C02F3/34C02F2103/10C12N1/20
Inventor 张春王玉建金吉中刘雅雪张思兰王朝强梅绪东刘璞何敏徐烽淋王丹熊德明
Owner 重庆市涪陵页岩气环保研发与技术服务中心
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