Cleavage culture solution and preparation method thereof

A technology of cleavage culture medium and salt solution, applied in the field of human assisted reproduction, can solve problems such as poor in vitro culture effect and inability to effectively ensure embryo development, and achieve the effects of ensuring safety, reducing toxicity, maintaining vitality and nutrition

Inactive Publication Date: 2018-11-02
瑞柏生物(中国)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the conventional cleavage culture medium used clinically is human tubal saline solution (HTF) with sodium bicarbonate as the buffer, which cannot effectively ensure the development of embryos, especially for in vitro culture of early embryo development.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described below in combination with specific implementation methods.

[0025] The cleavage culture solution of the present invention is made of alanine, aspartic acid, asparagine, glutamic acid, glutamine, glycine, proline, taurine, serine, threonine, tyrosine Amino acid, methionine, lipoic acid, hyaluronic acid and chelating agent are dissolved in a saline solution containing antibiotics and indicators. In the salt solution, alanine, aspartic acid, asparagine, glutamic acid, glutamine, glycine, proline, taurine, serine, threonine, tyrosine, methionine The concentration of acid is 0.02-1.50mmol / L; the concentration of hyaluronic acid is 0.01-0.50mg / mL; the concentration of lipoic acid is 0.01-0.30mmol / L; the chelating agent is ethylenediamine tetravalent carboxylic acid Tetraacetic acid, appropriate amount; the antibiotic is gentamicin, the concentration is 4-12 μg / mL; the indicator is phenol red, the concentration is 4.5-10.0 μg / m...

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PUM

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Abstract

The invention relates to a cleavage culture solution and a preparation method thereof and belongs to the technical field of human assisted reproductive technology. The cleavage culture solution has the advantages that the cleavage culture solution is prepared by adding alanine, aspartic acid, asparagine, glutamic acid, glutamine, glycine, proline, taurine, serine, threonine, tyrosine, methionine,lipoic acid, hyaluronic acid and a chelating agent into a salt solution, the activity and nutrition of an embryo can be kept effectively, the chelating agent can chelate some unnecessary heavy metal ions, the toxicity of the culture solution is lowered, the development of the early embryo can be promoted favorably, the success rate of the assisted reproductive technology is increased, and powerfulguarantee is provided for the safety of the assisted reproductive technology.

Description

technical field [0001] The invention relates to a cleavage culture solution and a preparation method thereof, belonging to the technical field of human assisted reproduction. Background technique [0002] In human assisted reproductive technology, cleavage medium is mainly used in the early culture process of embryos. At present, the conventional cleavage culture medium used clinically is human tubal saline solution (HTF) with sodium bicarbonate as a buffer, which cannot effectively ensure the development of embryos, especially for in vitro culture of early embryo development. Contents of the invention [0003] The purpose of the present invention is to provide a cleavage culture solution with various quality control standards, which can not only effectively maintain the vitality of embryos, improve the early embryonic development rate of assisted reproductive technology, but also provide a strong guarantee for the safety of assisted reproductive technology . [0004] In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0603C12N2500/32C12N2501/905
Inventor 冯怀亮
Owner 瑞柏生物(中国)股份有限公司
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