Cleavage culture solution and preparation method thereof
A technology of cleavage culture medium and salt solution, applied in the field of human assisted reproduction, can solve problems such as poor in vitro culture effect and inability to effectively ensure embryo development, and achieve the effects of ensuring safety, reducing toxicity, maintaining vitality and nutrition
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[0024] The present invention will be further described below in combination with specific implementation methods.
[0025] The cleavage culture solution of the present invention is made of alanine, aspartic acid, asparagine, glutamic acid, glutamine, glycine, proline, taurine, serine, threonine, tyrosine Amino acid, methionine, lipoic acid, hyaluronic acid and chelating agent are dissolved in a saline solution containing antibiotics and indicators. In the salt solution, alanine, aspartic acid, asparagine, glutamic acid, glutamine, glycine, proline, taurine, serine, threonine, tyrosine, methionine The concentration of acid is 0.02-1.50mmol / L; the concentration of hyaluronic acid is 0.01-0.50mg / mL; the concentration of lipoic acid is 0.01-0.30mmol / L; the chelating agent is ethylenediamine tetravalent carboxylic acid Tetraacetic acid, appropriate amount; the antibiotic is gentamicin, the concentration is 4-12 μg / mL; the indicator is phenol red, the concentration is 4.5-10.0 μg / m...
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