Tubular bacterial nanocellulose material and preparation method and application thereof

A technology of nanocellulose and bacteria, applied in biochemical equipment and methods, methods based on microorganisms, medical science, etc., can solve the problems of radial delamination, easy adhesion of platelets, uneven structure of composite materials, etc., and achieve blood The effect of good compatibility and excellent anticoagulant performance

Pending Publication Date: 2018-11-06
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The length of the BNC tube obtained by this method is controllable, but there is radial delamination
The third device is composed of an inner silicone tube and an outer glass tube. The fermentation broth is inoculated in the inner cavity of the inner silicone tube and the outer glass tube, and it is placed in an air or oxygen environment, and a BNC tube can be formed in the cavity. The formation of the BNC tube prepared by the device and method is similar to the second method, and there is also delamination
Although the dense inner surface of the BNC tube is conducive to the adhesion and spreading of cells, at the same time, platelets are also easier to adhere to, which promotes blood coagulation to a certain extent; The inner wall of the lumen introduces other polymers, resulting in uneven structure of the synthesized composite material

Method used

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  • Tubular bacterial nanocellulose material and preparation method and application thereof
  • Tubular bacterial nanocellulose material and preparation method and application thereof
  • Tubular bacterial nanocellulose material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Prepare 100mL of liquid medium (50g / L fructose, 5g / L peptone, 3g / L yeast powder) in a 250mL Erlenmeyer flask, sterilize at 115°C for 30min under high temperature and high pressure, cool to room temperature, and inoculate Gluconacetobacter xylinus ( Current name: Komagataeibacter xylinus) ATCC 23770 strain, or G. xylinus ATCC 23766, or G. xylinus ATCC 23769, 30 ° C, 100 rpm shaking culture for 24 hours, to obtain a fermented broth with vigorous strain.

[0039] (2) Prepare a glass rod with a length of 12 mm and a diameter of 3 mm, a silicone tube with a length of 12 mm, an inner diameter of 8 mm, and a wall thickness of 0.5 mm, and two cylindrical hollow silicone plugs with large and small heads. The diameter of the large end is 10 mm, and the diameter of the small end is 5mm, the hollow diameter is 3mm. Wash and dry, put the glass rod and the silicone tube coaxially in the lumen of the silicone tube, a hollow cylindrical cavity is formed between the glass rod and th...

Embodiment 2

[0043] (1) Prepare 100mL of liquid medium (100g / L glucose, 5g / L peptone, 3g / L yeast powder), place it in a 250mL Erlenmeyer flask, sterilize at 115°C for 30min, cool to room temperature, and The strain ATCC700178 was inoculated in a clean workbench, and cultured at 30° C. and 100 rpm on a shaker at a constant temperature for 24 hours to obtain a fermentation broth.

[0044] (2) Prepare a steel rod with a length of 12mm and a diameter of 2mm, a silicone tube with a length of 12mm, an inner diameter of 6mm, and a wall thickness of 0.5mm, and two cylindrical hollow silicone plugs with large and small heads. The diameter of the large end is 8mm, and the diameter of the small end is 5mm, the hollow diameter is 2mm. After washing and drying, the steel rod and the silicone tube are coaxially placed in the lumen of the silicone tube. A hollow cylindrical cavity is formed between the steel rod and the silicone tube. The opening of the silicone tube is sealed by a cylindrical hollow sil...

Embodiment 3

[0048] (1) Prepare 100mL of liquid medium (100g / L glucose, 5g / L peptone, 3g / L yeast powder), put it in a 250mL Erlenmeyer flask, sterilize at 115℃ for 30min, cool to room temperature, and inoculate ZCY The strain of Gluconacetoacetobacter xylinum ATCC23770 was cultured at 30° C. and shaken at 100 rpm for 24 hours to obtain a fermented liquid with vigorous strain.

[0049] (2) Prepare a glass rod with a length of 12 mm and a diameter of 3 mm, a silicone tube with a length of 12 mm, an inner diameter of 8 mm, and a wall thickness of 0.5 mm, and two cylindrical hollow silicone plugs with large and small heads. The diameter of the large end is 10 mm, and the diameter of the small end is 5mm, the hollow diameter is 3mm. After washing and drying, the glass rod and the silicone tube are coaxially placed in the lumen of the silicone tube. A hollow cylindrical cavity is formed between the glass rod and the silicone tube. The opening of the silicone tube passes through a cylindrical hol...

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PUM

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Abstract

The invention relates to a tubular bacterial nanocellulose material and a preparation method and application thereof. Fibers on the inner surface of the tubular bacterial nanocellulose material are loose, and fibers on the outer surface of the tubular bacterial nanocellulose material are dense. The preparation method comprises the following steps of fermentation broth preparation, device assemblyand tubular bacterial nanocellulose material preparation. The tubular bacterial nanocellulose material is applied to artificial blood vessels, artificial nerve conduits or artificial ureters. The fibers on the inner surface of the prepared tubular BNC (bacterial nanocellulose) material are loose, the fibers on the outer surface of the prepared tubular BNC material are dense, and the prepared tubular BNC material is a material different from previously reported tubular BNC material in structure, and is a biomaterial with a great application potential; the tubular bacterial nanocellulose material is an excellent substrate material for preparing a BNC composite pipe; and the blood compatibility of the inner surface of the tubular bacterial nanocellulose material is higher than that of reported products obtained by several other reactors.

Description

technical field [0001] The invention belongs to the field of cellulose material and its preparation method and application, in particular to a tubular bacterial nanocellulose material and its preparation method and application. Background technique [0002] Bacterial nanocellulose (BNC) is a kind of cellulose produced by bacteria, which has the same chemical composition as plant cellulose, but has high chemical purity, high water content, unique three-dimensional network structure, and good biocompatibility. It is widely used in food industry, cosmetics industry, electronics industry, biomedical materials and other fields. [0003] The structure and properties of BNC can be regulated by controlling the culture conditions. According to different culture methods, BNCs of different shapes can be prepared, including membranes, blocks, tubes, microspheres, etc. Tubular BNCs can be prepared by designing special devices. According to reports, there are currently many different ty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04A61L27/20A61L27/50C12R1/01
CPCA61L27/20A61L27/50A61L27/507A61L2430/22A61L2430/32C12P19/04C08L1/02
Inventor 洪枫包露涵唐敬玉汤曼季昌明陈琳
Owner DONGHUA UNIV
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