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Droplet chip cell analysis system and analysis method thereof

An analysis system and chip technology, applied in the field of medical devices, to achieve the effects of fast analysis, flexible application, and reduced reagent consumption

Active Publication Date: 2018-11-13
广州市第一人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no integrated system that can complete all cell analysis steps

Method used

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  • Droplet chip cell analysis system and analysis method thereof
  • Droplet chip cell analysis system and analysis method thereof
  • Droplet chip cell analysis system and analysis method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A droplet chip cell analysis system, comprising: an installation platform as a basic installation plane, a sample carrier platform for carrying cell analysis samples, a reagent carrier platform for cell analysis reagents, a chip carrier platform for open chips, and a sample carrier platform on the sample carrier platform , Reagent carrying platform, chip carrying platform between the sampling needle for micro pipetting, the temperature control module for temperature control of the chip, the optical detection module for cell analysis of the contents in the chip, and the signal acquisition for the optical detection module The signal acquisition module, the spatial translation mechanism, the linear translation mechanism, and the dark chamber where the optical detection module is installed; the sample carrier, reagent carrier, chip carrier, sampling needle, temperature control module, optical detection module, and signal acquisition module are all integrated in the On the in...

Embodiment 2

[0040] A droplet chip cell analysis system, comprising: an installation platform as a basic installation plane, a sample carrier platform for carrying cell analysis samples, a reagent carrier platform for cell analysis reagents, a chip carrier platform for open chips, and a sample carrier platform on the sample carrier platform , Reagent carrying platform, chip carrying platform between the sampling needle for micro pipetting, the temperature control module for temperature control of the chip, the optical detection module for cell analysis of the contents in the chip, and the signal acquisition for the optical detection module The signal acquisition module, the plane translation mechanism, the rotating lifting arm, and the dark chamber where the optical detection module is installed; the sample carrier, reagent carrier, chip carrier, sampling needle, temperature control module, optical detection module, and signal acquisition module are all integrated in the On the installation...

Embodiment 3

[0045] The analysis system of Embodiment 1 is used to realize the bacterial antibiotic susceptibility test based on the color reaction on the glass chip with patterned hydrophilic dots.

[0046] The experiment uses a glass material chip with a size of 5×10cm. The chip has a group of 6×12 hydrophilic lattices, each hydrophilic dot has a diameter of 3mm, and the area around the hydrophilic dot is treated with hydrophobic and oleophobic coating.

[0047] Specific steps are as follows:

[0048] (1) The sampling needle sucks 5 μL of FC-40 fluorinated oil and 5 μL of urine sequentially, and loads them on the hydrophilic spots of the column, forming a total of 72 droplets in 6 rows and 12 columns.

[0049] (2) 5 μL of culture medium containing six concentrations (0-5) of one antibiotic was added to each row of droplets. There were 12 kinds of antibiotics tested, including: erythromycin, clindamycin, ampicillin, vancomycin, gentamicin, ciprofloxacin, levofloxacin, imipenem, cefazoli...

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PUM

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Abstract

The invention discloses a droplet chip cell analysis system. The droplet chip cell analysis system comprises a mounting platform as a base mounting plane, a sample bearing platform for bearing a cellanalysis sample, a reagent bearing platform for bearing a cell analysis reagent, a chip bearing platform for bearing an open chip, a sampling needle for translating trace transferred liquid among thesample bearing platform, the reagent bearing platform and the chip bearing platform, a temperature control module for controlling the temperature of the chip, an optical detection module for cell analysis of contents in the chip as well as a signal acquisition module for signal acquisition of the optical detection module. The invention also comprises an analysis method for the droplet chip cell analysis system. The droplet chip cell analysis system based on a micro-droplet technology, provided by the invention, integrates the whole process of analysis of certain cells and belongs to a medicalinstrument for biological detection. The droplet chip cell analysis system has the advantages of operation automation, high analysis speed and high text flux.

Description

technical field [0001] The invention relates to a medical device for biological detection, in particular to a droplet chip cell analysis system and an analysis method thereof. Background technique [0002] Compared with traditional biological analysis using test tubes as carriers, droplet analysis has many advantages: (1) The droplet reactor is small in size, which can significantly save reagent consumption; (2) It is easy to achieve high-throughput analysis; (3) Benefiting from the shortening of the reaction time in the microsystem, the analysis time can be significantly saved; (4) the water phase sample is wrapped by the oil phase, which eliminates the influence of evaporation and surface adsorption factors on the analysis results. [0003] Typical cell analysis steps include: cell seeding, cell culture, cell stimulation, and cell response detection. In the prior art, there is no integrated system that can complete all cell analysis steps. Contents of the invention [...

Claims

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Application Information

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IPC IPC(8): G01N35/00G01N35/10G01N21/64
CPCG01N21/6458G01N35/00584G01N35/1011G01N2035/1037
Inventor 刘大渔
Owner 广州市第一人民医院