Extraction method for sheep serum albumin

Abstract

The invention provides an extraction method for sheep serum albumin. The method comprises the following steps: (1) fresh sheep blood is taken, an anticoagulant accounting for 12% of the original serumvolume is added, after qualified inspection and quarantine, centrifugation is performed at 4 DEG C and 2500 rpm for 15 min, a supernatant is taken and frozen at subzero 20 DEG C, frozen serum is taken, thaws sufficiently at room temperature and is centrifuged at 10000 rpm for 12 min, and a supernatant is taken and is the sheep serum. The extraction method for the sheep serum albumin is short in flow, simple to operate and high in production efficiency, no organic solvent is used in the method, protein aggregation or denaturation is avoided, meanwhile, environmental pollution and unsafe factors in the production process can be reduced, product purity is improved, and the method can be widely applied to the fields of medical treatment, biochemistry and the like.

Description

technical field [0001] The invention relates to the field of serum albumin extraction, in particular to a method for extracting sheep serum albumin. Background technique [0002] Protein is the material basis of life. Serum albumin is the most abundant protein in plasma. It can combine with many endogenous and exogenous compounds to play the role of storage and transformation. In the serum-free culture of animal cells, adding albumin can play the role of physiological and mechanical protection and carrier. Sheep serum albumin (BSA), also known as the fifth component, is a globulin in sheep serum, containing 583 amino acid residues, with a molecular weight of 66.43kDa and an isoelectric point of 4.7. Biochemical reagents widely used in other fields. [0003] At present, the extraction of sheep serum albumin mainly has the following three methods. The salting-out method uses ammonium sulfate fractional precipitation and is refined by octanoic acid treatment. This method is ...

AI Technical Summary

Problems solved by technology

The salting-out method uses ammonium sulfate fractional precipitation and is refined by octanoic acid treatment. This method is the most traditional production method, but its process is cumbersome and the production efficiency is not high; the organic solvent precipitation method utilizes the low dielectric constant properties of ethanol and protein Under the conditions of certain temperature, pH, ionic strength and concentration, the solubility in different concentrations of ethanol is different, so as to realize the separation of plasma proteins. This method can produce a variety of plasma proteins, but the purity of the proteins is not high; the heat shock method is to take Goat blood is anticoagulated with sodium citrate and centrifuged to obtain plasma, heated, added sodium caprylate, added ethanol after adjusting the pH value, and then obtained sheep serum albumin after multi-stage treatment. In this method, some chemical reagents will remain in serum albumin , resulting in lower purity of sheep serum albumin