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Stylonychia lemnae pure culture system and preparation method and culture method thereof

A technique of pure culture of Acanthoplasses, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of unfavorable basic biological research of Acanthus, bacteria and microalgae are difficult to be completely digested, and cannot be realized Questions about pure culture of Acanthus cells

Active Publication Date: 2018-11-16
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because Acanthus is carnivorous, bacteria and microalgae are still difficult to be completely digested in the worms even if they are starved before the experiment.
However, if a large number of cultured cells need to be fed with lip whipworms, the mixture of lip whipworms and acanthus cells in large numbers in the culture medium cannot achieve pure culture of acanthus cells, which is very unfavorable for the basic biological research of acanthus

Method used

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  • Stylonychia lemnae pure culture system and preparation method and culture method thereof
  • Stylonychia lemnae pure culture system and preparation method and culture method thereof
  • Stylonychia lemnae pure culture system and preparation method and culture method thereof

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specific Embodiment approach 1

[0022] Specific embodiment one: a kind of duckweed pure culture system of Acanthus duckweed of the present embodiment, it is made up of amino acid, nucleic acid composition, vitamin, inorganic component, organic acid and glucose;

[0023] Among them, the amino acid components in each 100mL of pure culture system of Acanthus duckweed are 20-30 mg of L-alanine, 15-20 mg of L-lysine, 20-30 mg of L-arginine, 20 ~30mg of L-methionine, 20~35mg of L-cysteine, 20~30mg of DL-phenylalanine, 25~40mg of L-glutamic acid, 15~20mg of L-proline, 5 ~10mg of L-glutamine, 20~30mg of DL-serine, 25~40mg of glycine, 25~40mg of L-threonine, 20~35mg of L-leucine, 15~20mg of L-tryptophan acid, 10-20mg of L-valine and 20-35mg of L-tyrosine;

[0024] The nucleic acid composition in each 100mL pure culture system of Acaneus duckweed is composed of 3-5 mg of adenosine, 7-9 mg of guanosine, 3-5 mg of cytidine and 3-5 mg of uracil;

[0025] The vitamins in each 100mL of pure culture system of Acanthus duc...

specific Embodiment approach 2

[0028] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the amino acid components in the pure culture system of Acaneus duckweed per 100 mL are 22.5 mg of L-alanine and 17.5 mg of L-lysine acid, 22.5mg of L-arginine, 22.5mg of L-methionine, 35mg of L-cysteine, 22.5mg of DL-phenylalanine, 27.5mg of L-glutamic acid, 17.5mg of L - Proline, L-Glutamine 8mg, DL-Serine 22.5mg, Glycine 27.5mg, L-Threonine 27.5mg, L-Leucine 35mg, L-Tryptophan 17.5mg, Composed of 17.5mg of L-valine and 35mg of L-tyrosine. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0029]Specific embodiment three: the difference between this embodiment and specific embodiment one is: the nucleic acid composition in the pure culture system of Acaneus duckweed per 100mL is composed of 4 mg of adenylic acid, 8 mg of guanosine, 4 mg of cytidine and 4mg of uracil composition. Others are the same as in the first embodiment.

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Abstract

A Stylonychia lemnae pure culture system and a preparation method and a culture method thereof relate to a pure culture system and a preparation method and a culture method thereof. The invention aimsto solve the problem that there is no effective culture system for aseptic pure culture of Stylonychia lemnae at present. The culture system of the invention is composed of amino acid, nucleic acid composition, vitamins, inorganic component, organic acid and glucose. By using a Tetrahymena thermophila pure medium as the reference and through optimization of composition ratio thereof, aseptic pureculture of Stylonychia lemnae is realized. By optimizing the culture condition and establishing an efficient economic cell pure culture system, enlarged pure culture theoretical basis and technical support will be provided for the molecular biology study of Stylonychia lemnae. The invention is applied in the field of Stylonychia culture.

Description

technical field [0001] The invention relates to a pure culture system, a preparation method and a culture method thereof. Background technique [0002] Duckweed Acanthus (Stylonychia lemnae) is a common free-living freshwater ciliate, belonging to the genus Acanthus genus of the Chaeteaceae Achyceraceae under the Ciliary Phylum Polymembrane of the Protozoa Kingdom, and is widely distributed in ponds, rivers and swamps middle. As a single-celled model organism, Acanthocercia has a long and rich history in the study of nuclear organization and development, chromosomes and chromatin, telomere biology, and genome rearrangement. As early as 1876, the first reports documenting chromosomes and the mitotic spindle came from observing the structure of the small nucleus of Acanthoceres. The phenomenon of massive DNA loss (approximately 90%) in the genome following macrokaryotic polyploidization in Acanthocerca duckweed has stimulated the study of genome reduction and rearrangement i...

Claims

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Application Information

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IPC IPC(8): C12N1/10C12R1/90
CPCC12N1/10
Inventor 陈瑛姚琳王丽潘旭明王影范金凤李世宽蔡兴隆杜明磊王香郑维彬葛畅潘蒙蒙王重女张雨楠于淼
Owner HARBIN NORMAL UNIVERSITY
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