Method for producing biosurfactant through strain fermentation

A biological surface and active agent technology, applied in the biological field, can solve problems such as the loss of bacteria and nutrients, affect the conversion efficiency, and reduce the production of surfactants, and achieve the effects of reducing separation costs, simple fermentation methods, and increased product concentration

Inactive Publication Date: 2018-11-20
董一骅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biosurfactant itself has a good foaming effect, and a large amount of foam will be generated under the condition of ventilation and stirring, and the nutrients and bacteria in the fermentation broth will be taken out as the air is discharged, resulting in the growth of bacteria in the culture medium. A large amount of nutrients and nutrients are lost, which affects the conversion efficiency, resulting in low substrate utilization and reduced surfactant production.
Measures such as stirring and aeration during the fermentation process will cause the fermentation liquid to overflow in the form of foam, which not only loses the nutrients and bacteria of the medium, but also easily contaminates miscellaneous bacteria

Method used

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  • Method for producing biosurfactant through strain fermentation
  • Method for producing biosurfactant through strain fermentation
  • Method for producing biosurfactant through strain fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 This example illustrates the rhamnolipid fermentation process in a conventional stirred fermenter.

[0016] Strain: Pseudomonas aeruginosa YM4.

[0017] Seed medium: xylose 15g / L; nitrogen source (sodium nitrate, ammonium nitrate, ammonium chloride, ammonium sulfate, urea or yeast powder); KH 2 PO 4 4g / L; K 2 HPO 4 ·3H 2 O 4g / L; NaCl 1g / L; KCl 1g / L; MgSO 4 ·7H 2 O 0.2g / L; CaCl 2 0.1g / L.

[0018] Fermentation medium: soybean oil 30g / L; NaNO 3 6g / L; KH 2 PO 4 4g / L; K 2 HPO 4 ·3H 2 O 4g / L; NaCl 1g / L; KCl 1g / L; MgSO 4 ·7H 2 O 0.2g / L; CaCl 2 0.1g / L; trace elements 2.5mL / L (FeCl 3 0.16g / L; CuSO 4 0.15g / L; ZnSO 4 ·7H 2 O 1.5g / L; MnSO 4 ·H 2 O 1.5g / L).

[0019] Shake flask seed culture: Take a loop of bacteria from the slant medium, inoculate it into a 250mL Erlenmeyer flask containing 50mL of seed medium, and incubate in a shaking incubator at 37°C and 200rpm for 20h.

[0020] 5L fermenter culture: inoculate the seed culture solution in...

Embodiment 2

[0024] Example 2 This example illustrates the production of rhamnolipids by fermentation in a high-oxygen air medium.

[0025] Strain: Pseudomonas aeruginosa YM4.

[0026] Seed medium: xylose 15g / L; nitrogen source (sodium nitrate, ammonium nitrate, ammonium chloride, ammonium sulfate, urea or yeast powder); KH 2 PO 4 4g / L; K 2 HPO 4 ·3H 2 O 4g / L; NaCl 1g / L; KCl 1g / L; MgSO 4 ·7H 2 O 0.2g / L; CaCl 2 0.1g / L.

[0027] Fermentation medium: soybean oil 30g / L; NaNO 3 6g / L; KH 2 PO 4 4g / L; K 2 HPO 4 ·3H 2 O 4g / L; NaCl 1g / L; KCl 1g / L; MgSO 4 ·7H 2 O 0.2g / L; CaCl 2 0.1g / L; trace elements 2.5mL / L (FeCl 3 0.16g / L; CuSO 4 0.15g / L; ZnSO 4 ·7H 2 O 1.5g / L; MnSO 4 ·H 2 O 1.5g / L).

[0028] Shake flask seed culture: Take a loop of bacteria from the slant medium, inoculate it into a 250mL Erlenmeyer flask containing 50mL of seed medium, and incubate in a shaking incubator at 37°C and 200rpm for 20h.

[0029] Gas medium with high concentration of oxygen: Use an oxyge...

Embodiment 3

[0034] Example 3 This example illustrates the use of foam separation to concentrate rhamnolipids in the late stage of bacterial strain fermentation to produce rhamnolipids

[0035] Replace the high-oxygen gas in the fermentation system fermented for 48 hours in Example 2 with ordinary air, connect a hose and a collection device to the exhaust port, adjust the ventilation to 1.5vvm, stir at 200rpm, and maintain for 1.5h-2h Until there is no more foam overflow at the vent of the fermenter, collect the foam overflow, measure the volume and analyze the rhamnolipid content. The results are shown in Table 3. Before the end of fermentation, ordinary air medium is used for foam separation. The rhamnolipid content in the foam overflow liquid reaches about 25g / L, and there is no more rhamnolipid in the raffinate in the fermenter, realizing the rhamnolipid separation. concentrate.

[0036] Table 3 uses foam separation technology to the concentration effect of rhamnolipid fermentation brot...

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Abstract

The invention relates to a method for producing a biosurfactant through strain fermentation. A gas medium with high oxygen content supplies oxygen to a fermentation system, so that the ventilation volume and a stirring speed of a fermentation tank are substantially reduced, and an overflow-free fermentation process of the biosurfactant is realized in a conventional stirring reactor; the method hasthe advantages of high production rate, high substrate conversion rate and the like; in the last phase of fermentation, general air is used as a medium; a product in fermented liquid is concentratedby adopting a foam separation technology; a new process beneficial to industrial production of the biosurfactant is provided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for bacterial strain fermentation to produce biosurfactants. Background technique [0002] Biosurfactants, including structures such as glycolipids and lipopeptides, such as rhamnolipids and surfactin, are the most widely studied types of biosurfactants in recent years. It not only has the properties of solubilization, emulsification, wetting, and foaming comparable to chemical surfactants, but also has the characteristics of low toxicity, easy biodegradation, and environmental friendliness, making it a potential synthetic surfactant. Substitutes, so it has great application potential in fields such as petroleum exploration, medicine, food, daily chemical, and environmental protection. [0003] During the production of biosurfactants, the production strain needs to ingest dissolved oxygen to carry out the fermentation pathway that is beneficial to the synthesis of metabolit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P19/44C12R1/125C12R1/385
CPCC12P19/44C12P21/00
Inventor 董一骅
Owner 董一骅
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