Bacillus megatherium BM22 and microcapsule preparation and application thereof

A technology of Bacillus megaterium and BM22, which is applied in the field of microorganisms to achieve good control effect, long storage time and good stress resistance.

Active Publication Date: 2018-11-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are reports on the pathogen, occurrence and harm, and chemical control of pepper gum disease, but the biological control that is environmentally friendly, safe for humans and animals, and sustainable use has not been involved. The research on the biological control of pepper gum disease and its preparations still remains blank

Method used

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  • Bacillus megatherium BM22 and microcapsule preparation and application thereof
  • Bacillus megatherium BM22 and microcapsule preparation and application thereof
  • Bacillus megatherium BM22 and microcapsule preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and Identification of Bacillus megaterium BM22

[0027] 1.1 Isolation of Bacillus megaterium BM22

[0028] Healthy leaves of Eucommia ulmoides were collected from the black spot occurrence area of ​​Eucommia ulmoides in Dayi, Sichuan. Rinse the collected samples with sterile water, weigh 1.0g of tissue, soak in 70% alcohol for 1-2min, then disinfect with 1-3% sodium hypochlorite solution for 3-5min, wash several times with sterile water, absorb Apply 100 μL of washing liquid for the last time to NA plate (3g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar powder, 1000mL distilled water, pH 7.0, after mixing and dispensing, autoclave at 121°C for 30min), culture in the dark at 27°C 24h, used as a control for surface disinfection, to check whether the surface disinfection is thorough.

[0029] Cut the sterilized leaves into pieces, put them into a sterile mortar, add sterilized quartz sand and 10mL of sterile water, grind them thoroughly, ...

Embodiment 2

[0040] The making of embodiment 2 bacillus megaterium BM22 microcapsule preparation

[0041] According to the Bacillus megaterium BM22 obtained in Example 1, microcapsule preparations are made through fermentation and drying, specifically comprising the following steps:

[0042] 1) Fermentation liquid: after autoclaving the nutritious meat culture liquid, bottle it according to the proportion of 100mL liquid in a 300mL triangular flask (oxygenation control), inoculate Bacillus megaterium BM22 slant seeds in a sterile state, and inoculate 2 slant planes per bottle Seeds were cultivated at a temperature of 28-30° C., an initial pH value of 6.5, and shaken at 120 r / min for 36 hours to prepare a Bacillus megaterium BM22 fermentation broth. Wherein the nutrient meat culture medium is: beef extract 7g, peptone 10g, NaCl 5g, water 1000mL.

[0043] 2) Microcapsule preparation: 8% of calcium lignosulfonate, 5% of polyethylene glycol, and 6% of oxalic acid are put into a 300mL wide-ang...

Embodiment 3

[0046] Example 3 The preventive experiment of Bacillus megaterium BM22 microcapsule preparation on prickly ash gum disease

[0047] 1) Preparation of BM22 microcapsule preparation dilution: Dilute 1 g of the microcapsule preparation prepared above with 50, 100, 200, 400, 800, 1600, and 2400 mL respectively to prepare a dilution.

[0048] 2) In the non-infested Zanthoxylum bungeanum, smear the branches with the dilution of the preparation in 1) once every spring, 50mL for 1-2 years old, 80mL for 3-5 years old, 100-150mL for more than 5 years old, and clean water as a control.

[0049] 3) 30 days after smearing, carry out disease investigation and statistics according to the disease grading standard in Table 3, and calculate the incidence rate, disease index and control effect, and the results are shown in Table 4.

[0050] Table 3 Grading standard of pepper gum disease

[0051]

[0052] Incidence rate (%)=(number of diseased plants / sum of number of plants)×100;

[0053] Di...

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Abstract

The invention discloses bacillus megatherium BM22 and a microcapsule preparation and application thereof. The strain is collected in China General Microbiological Culture Collection Center, and has acollection number of CGMCC No. 15070. The microcapsule preparation taking the bacillus megatherium BM22 as an active ingredient is prepared by the following steps: culturing by a seed solution, and culturing and fermenting in a mixture of a carrier, an assistant and a protective agent, and drying, thereby obtaining the product. The bacillus megatherium BM22 and the microcapsule preparation thereofprovided by the invention have excellent effects of preventing gumming disease of Sichuan pepper. When branches of Sichuan pepper free of the disease are smeared with a 800-1600-times diluent of thepreparation once in spring, the gumming disease of Sichuan pepper can be prevented; and after gummosis is removed from diseased Sichuan pepper in spring and autumn per year, a 400-800-times diluent ofthe preparation is applied to the scab, and the gumming disease of Sichuan pepper can be effectively treated.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a bacillus megaterium, in particular to a bacillus megaterium BM22 strain, a microcapsule preparation thereof and an application in treating prickly ash gum disease. Background technique [0002] Zanthoxylum bungeanum is a deciduous shrub or small tree belonging to Rutaceae and Zanthoxylum genus. It is the main economically cultivated species of Zanthoxylum bungeanum in the world. It has a long history of cultivation and use in my country, with many varieties and resources and a wide range of cultivation. Zanthoxylum bungeanum has many bioactive components and has high medicinal, edible and ecological values. Its fruit is round and about the size of a mung bean. Its outer skin is a commonly used spice, which can extract aromatic oil and be used as medicine. The seeds are edible and can also be processed to make soap. In terms of medicinal value, it is widely used in the research ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N25/28A01N63/00A01N63/02A01P3/00C12R1/11
CPCA01N25/28A01N63/00A01N63/10C12N1/20C12N1/205C12R2001/11
Inventor 李姝江曾艳玲黎肇家朱天辉
Owner SICHUAN AGRI UNIV
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