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Method for preparing pravastatin-loading vascular tissue engineering scaffold material by electrospinning

An electrospinning method and vascular tissue technology, applied in the field of fibrous artificial vascular materials, can solve the problems of non-degradable, non-degradable, and only 1 to 7 days, and achieve the effect of easy regulation, simple method, and prevention and treatment of recurrence

Active Publication Date: 2018-11-30
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the patent uses statin molecular prodrugs, which need to be hydrolyzed into statins in the body by ester hydrolase to exert their efficacy. into the material, because the material cannot be degraded in the body, it needs to be taken out again
The patent application with the publication number CN101856342A discloses a pravastatin transdermal administration preparation, which mixes pravastatin with a transdermal enhancer, a reservoir matrix composed of synthetic polymers and / or modified cellulose Obtain drug reservoirs and prepare transdermal drug formulations, but the materials used in this dosage form are also basically non-degradable materials, and the sustained drug release time is only 1 to 7 days

Method used

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  • Method for preparing pravastatin-loading vascular tissue engineering scaffold material by electrospinning
  • Method for preparing pravastatin-loading vascular tissue engineering scaffold material by electrospinning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of scaffold materials for vascular tissue engineering

[0024] (1) Add poly(3-hydroxybutyrate-3-hydroxyhexanoate) into dichloromethane, heat and magnetically stir in a water bath at 30°C for 24 hours, and prepare a polyhydroxyalkanoate solution with a mass concentration of 7%, statically Remove air bubbles after placing;

[0025] (2) Add pravastatin sodium in acetic acid, stir magnetically at 25° C., and make a pravastatin sodium solution with a concentration of 20 mg / mL;

[0026] (3) According to the volume ratio of polyhydroxyalkanoate solution: pravastatin sodium solution=1:1, under the condition of mixing slowly while stirring, the polyhydroxyalkanoate solution and pravastatin sodium solution are mixed uniformly, Obtain an electrospinning solution;

[0027] (4) Put the electrospinning solution obtained in step (3) into an electrospinning device for electrospinning to obtain micro-nano fibers, and dry them in vacuum at 60° C. for 24 hours to obtain t...

Embodiment 2

[0031] 1. Preparation of scaffold materials for vascular tissue engineering

[0032] (1) Add poly(3-hydroxybutyrate-3-hydroxyvalerate) into a mixed solvent of chloroform and DMF, heat and magnetically stir in a water bath at 50°C for 24 hours, and prepare a polyhydroxyalkane with a mass concentration of 30%. Ester solution, remove bubbles after leaving standstill; Wherein the mixed volume ratio of chloroform and DMF in the mixed solvent is 8: 2;

[0033] (2) Add pravastatin sodium to isopropanol, stir magnetically at 40° C., and prepare a pravastatin sodium solution with a concentration of 300 mg / mL;

[0034] (3) According to the volume ratio of polyhydroxyalkanoate solution: pravastatin sodium solution=8:1, under the condition of mixing slowly while stirring, the polyhydroxyalkanoate solution and pravastatin sodium solution are mixed uniformly, Obtain an electrospinning solution;

[0035] (4) Put the electrospinning solution obtained in step (3) into an electrospinning devi...

Embodiment 3

[0039] 1. Preparation of scaffold materials for vascular tissue engineering

[0040] (1) Add poly(3-hydroxybutyrate-4-hydroxybutyrate) into chloroform, heat and magnetically stir in a water bath at 40°C for 12h, and prepare a polyhydroxyalkanoate solution with a mass concentration of 20%, statically Remove air bubbles after placing;

[0041] (2) Pravastatin sodium is added in the mixed solvent of ethanol and DMF, under 25 ℃ magnetic stirring, be made into the pravastatin sodium solution that concentration is 100mg / mL; Wherein the volume ratio of ethanol and DMF in the said mixed solvent is 1:1;

[0042] (3) According to the volume ratio of polyhydroxyalkanoate solution:pravastatin sodium solution=6:1, under the condition of mixing slowly while stirring, the polyhydroxyalkanoate solution and pravastatin sodium solution are mixed evenly, Obtain an electrospinning solution;

[0043] (4) Put the electrospinning solution obtained in step (3) into an electrospinning device for el...

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Abstract

The invention relates to a method for preparing a pravastatin-loading vascular tissue engineering scaffold material by electrospinning. The method comprises: (1) preparing a polyhydroxyalkanoate solution; (2) preparing a pravastatin sodium solution; 3) preparing an electrospinning solution; and (4) electrospinning to obtain a vascular tissue engineering scaffold material. According to the presentinvention, the hydrophilic pravastatin sodium is loaded into the hydrophobic polyhydroxyalkanoate matrix, such that the prepared vascular tissue engineering scaffold material can achieve the long-acting release of pravastatin.

Description

technical field [0001] The invention relates to a prosthetic material, in particular to a fibrous artificial blood vessel material loaded with statin drugs. Background technique [0002] With the development of society and economy, cardiovascular disease has become the leading cause of death for human beings, and the annual treatment costs related to cardiovascular disease are huge. At present, the clinical surgical treatment of vascular diseases often uses autologous transplantation, allogeneic or xenografting, and artificial materials for vascular reconstruction. However, the supply of autologous transplantation is insufficient, and allogeneic or xenografting is prone to rejection infection or ethical issues, and the current artificial materials are not available. It has physiological functions and is prone to problems such as stenosis, embolism, calcium deposition or infection. [0003] In tissue engineering, autologous cells are planted on degradable scaffold materials ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/54A61L27/18D04H1/728
CPCA61L27/18A61L27/50A61L27/507A61L27/54A61L2300/216A61L2300/422A61L2300/602A61L2430/22D04H1/728C08L67/04
Inventor 卢玲蔡开灿李幸萍赵鹏胡翔陈智如
Owner SOUTHERN MEDICAL UNIVERSITY
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