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Method for preparing biological-bacteria fertilizer by utilizing tobacco leaf residues

A technology of residue and tobacco leaves, which is used in the preparation of compound biological bacterial fertilizer and the comprehensive utilization of waste tobacco leaf residues, can solve the problem of unclear changes in microbial interactions, species and bacterial species, and it is difficult to effectively control the number and proportion of bacterial species. , complex formula and other problems, to achieve the effect of a wide range of cheap raw materials, low price, good sensory smell

Active Publication Date: 2018-11-30
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the active substances of tobacco leaves have not been extracted, which has greatly caused a waste of resources
The production process used is mainly composting, the formula is complex, and many auxiliary materials need to be added, which takes a long time, usually 1 to 2 months
The common problem in this composting production method is that the interaction of microorganisms during the production process, the changes in the types and the number of bacteria are not clear, and it is difficult to effectively control the final number and proportion of bacteria

Method used

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  • Method for preparing biological-bacteria fertilizer by utilizing tobacco leaf residues
  • Method for preparing biological-bacteria fertilizer by utilizing tobacco leaf residues
  • Method for preparing biological-bacteria fertilizer by utilizing tobacco leaf residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of microbial seed solution

[0049] (1) Take 1 mL of Bacillus subtilis cultured in LB medium for 20 hours and inoculate it into 100 mL of Bacillus subtilis seed culture medium for expansion culture, 200 r / min, 37 ° C shaker culture for 20 hours, OD 620 When it reaches 4-6, it is Bacillus subtilis seed liquid.

[0050] (2) Take 1 mL of Bacillus colioids cultured in LB medium for 20 hours and inoculate it into 100 mL of Bacillus colioids seed culture medium for expansion culture, 200 r / min, 30 °C shaker culture for 20 hours, OD 620 When it reaches 4-6, it is the seed liquid of Bacillus colloidus.

[0051] (3) Take 1 mL of Paecilomyces lilacinus cultured in PDA medium for 20 hours and inoculate it into 100 mL of Paecilomyces lilacinus seed culture medium for expanded culture, and culture it on a shaker at 200 r / min at 30 °C for 20 hours, OD 620 When it reaches 4-6, it is the seed liquid of Paecilomyces lilacinus.

[0052] (4) The three kinds of seed solution...

Embodiment 2

[0054] Effect of Tobacco Leaf Raw Material on Bacterial Growth

[0055] (1) Take 60g of dried tobacco leaf residue and mix them in a 500mL Erlenmeyer flask according to the solid-liquid ratio of residue to water of 1:1.8 (mass ratio) to prepare tobacco leaf culture medium. Insert the Bacillus subtilis seed solution with a total mass of 10%, put it in a 37°C incubator and culture it for 4 days, and turn the material every 48 hours. The number of viable bacteria and the number of spores were counted on the plate culture. The number of viable bacteria and the number of spores were 2.5×10 13 cfu / g and 5×10 10 cfu / g.

[0056] (2) Grinding the untreated dried tobacco leaves as the raw material of the tobacco leaf culture medium, and carrying out the fermentation of Bacillus subtilis according to the above method (1). The culture time was 5 days and 7 days, and the number of viable bacteria obtained was 2.15×10 8 cfu / g and 6.37×10 8 cfu / g, the number of spores were 4.68×10 8 c...

Embodiment 3

[0059] Comparison of single-strain culture and compound-strain culture

[0060] (1) Put 35g of dried tobacco leaf residue and 49mL of water into a 250mL narrow-necked Erlenmeyer flask, and sterilize at 115°C for 20min. Insert the Bacillus subtilis seed solution with a total mass of 10%, and culture it statically in a 30°C incubator, and turn the material once every 48h. Both the number of viable bacteria and the number of spores reached the maximum on the 10th day, and the number of viable bacteria was 7×10 13 cfu / g, the number of spores is 1.5×10 13 cfu / g.

[0061] (2) Put 35 g of dried tobacco leaf residue and 49 mL of water into a 250 mL conical flask, and sterilize at 115° C. for 20 min. Insert 10% of the total mass of Bacillus colioids seed liquid, culture it statically in a 30°C incubator, and turn the material every 48 hours. Both the number of viable bacteria and the number of spores reached the maximum on the 12th day, and the number of viable bacteria was 2×10 1...

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Abstract

The invention discloses a method for preparing biological-bacteria fertilizer by utilizing tobacco leaf residues. The method comprises the following steps: taking residues obtained after active ingredients are extracted from dry tobacco leaves or fresh tobacco leaves as a culture medium, adding seed fluid of bacillus subtilis, bacillus mucilaginosus and paecilomyces lilacinus, uniformly mixing andcarrying out solid culture for 7 to 15 days to obtain corresponding biological-bacteria fertilizer, wherein the number of spores exceeds 109cfu / g. According to the method disclosed by the invention,tobacco leaf residues obtained after effective components are extracted are taken as the solid culture medium, and no other nutrients are needed, so that the production cost is reduced; microorganismsin the product exist in a form of spores and can be stably preserved for a long time, so that the microbial activity of the product is ensured. The product has abundant beneficial microorganisms andorganic matters and has the effects that the content of soil organic matters can be improved, the microecological environment of the soil is improved, the use amount of fertilizer is reduced, pests and diseases are reduced, and the quality and yield of crops are improved.

Description

technical field [0001] The invention belongs to the technical field of biological agriculture and relates to the preparation of a compound biological bacterial fertilizer and the comprehensive utilization of waste tobacco residues. Background technique [0002] my country's tobacco leaf production spreads all over the country, with an annual output of 4 to 5 million tons of tobacco leaves, and an annual total of 900,000 to 1 million tons of tobacco waste, accounting for about 18% to 25% of the total output. In addition, the number of field fresh tobacco leaves that are discarded or unqualified due to climate, pests, or improper management is also huge. Therefore, the comprehensive utilization of tobacco waste has become one of the important research directions of the domestic tobacco industry. Tobacco leaves are rich in protein, sugar, lignin, cellulose, organic acids, pectin, etc., and the content of nitrogen, phosphorus and potassium is significantly higher than that of gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125C12R1/07C12R1/79
CPCC12N1/20
Inventor 戴建英杨宇董悦生修志龙
Owner DALIAN UNIV OF TECH
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