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Upconversion magnetically-coded microspheres for mycotoxin flux detection and preparation method thereof

A mycotoxin and magnetic encoding technology, which is applied in the direction of microsphere preparation, microcapsule preparation, measuring device, etc., can solve the problems of increased health hazard coefficient, shortened analysis time, accumulation of mycotoxin, etc., and achieves stable optical properties and fluorescent background Low, high signal-to-noise ratio effect

Inactive Publication Date: 2018-12-04
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if some mycotoxins do not contaminate the same food crop at the same time, food processing often processes multiple raw materials into one food, resulting in the accumulation of mycotoxins. When people consume these foods, they may ingest multiple mycotoxins at the same time, and these toxins may There is an additive effect of toxicity, and the hazard coefficient to health increases
Therefore, it is urgent to establish a new method for accurate, sensitive, and high-throughput detection of mycotoxins to realize the simultaneous detection of multiple mycotoxins, shorten the analysis time, and avoid missed detections during the detection process that may cause harm to human health.

Method used

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  • Upconversion magnetically-coded microspheres for mycotoxin flux detection and preparation method thereof
  • Upconversion magnetically-coded microspheres for mycotoxin flux detection and preparation method thereof
  • Upconversion magnetically-coded microspheres for mycotoxin flux detection and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0111] (1) The synthesis steps of up-conversion nanoparticles are as follows:

[0112] 1) Up-conversion nanoparticles are prepared by solvothermal method: according to the mass ratio of yttrium chloride: ytterbium chloride: erbium chloride = 80:20:2, the rare earth solution is added to the reaction vessel, the heating temperature is set to 100°C and Stir until a white solid appears;

[0113] 2) After the water is completely evaporated to dryness, turn off the heating and cool to 50°C, add 5ml of oleic acid and 12ml of octadecene, and raise the temperature to 150°C to completely dissolve the white solid;

[0114] 3) Cool to 50°C, dissolve 0.1g of sodium hydroxide and 0.16g of ammonium fluoride in methanol solution, and add them into the rare earth reaction vessel; raise the temperature to 120°C, and vacuumize for 20 minutes with an oil pump;

[0115] 4) heating up to 280°C, stirring and reacting for 60 minutes;

[0116] 5) After the reaction is completed, alcohol is added for...

Embodiment 2

[0124] (1) The synthesis steps of up-conversion nanoparticles are as follows:

[0125] 1) The up-conversion nanoparticles are prepared by solvothermal method: according to the mass ratio of yttrium chloride: ytterbium chloride: thulium chloride = 75:20:2, the rare earth solution is added to the reaction vessel, the heating temperature is set to 110°C and Stir until a white solid appears;

[0126] 2) After the water is completely evaporated to dryness, turn off the heating and cool to 50°C, add 6ml of oleic acid and 12ml of octadecene, and raise the temperature to 130°C to completely dissolve the white solid;

[0127] 3) Cool to 50°C, dissolve 0.1g of sodium hydroxide and 0.15g of ammonium fluoride in methanol solution, and add them into the rare earth reaction vessel; raise the temperature to 120°C, and use an oil pump to evacuate for 30 minutes;

[0128] 4) heating up to 280°C, stirring and reacting for 60 minutes;

[0129] 5) After the reaction is completed, alcohol is add...

Embodiment 3

[0137] (1) The synthesis steps of up-conversion nanoparticles are as follows:

[0138] 1) The up-conversion nanoparticles are prepared by solvothermal method, which is an ABA three-layer structure: according to the mass ratio of yttrium chloride: ytterbium chloride: thulium chloride = 80:20:2, the rare earth solution is added to the reaction vessel, and the Heat the temperature to 110°C and stir until a white solid appears;

[0139] 2) After the water is completely evaporated to dryness, turn off the heating and cool to 40°C, add 4ml of oleic acid and 10ml of octadecene, and raise the temperature to 150°C to completely dissolve the white solid;

[0140] 3) Cool to 40°C, dissolve 0.15g of sodium hydroxide and 0.2g of ammonium fluoride in methanol solution, and add them into the rare earth reaction vessel; raise the temperature to 150°C, and vacuumize for 30 minutes with an oil pump;

[0141] 4) heating up to 270°C, stirring and reacting for 60 minutes;

[0142] 5) After the r...

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Abstract

The invention relates to upconversion magnetically-coded microspheres for mycotoxin flux detection and a preparation method thereof. The upconversion magnetically-coded microspheres used for simultaneous detection of various mycotoxins have a diameter of 2-8 microns. By irradiation with near-infrared light of 980 nm, different upconversion ion coded microspheres can emit different colors of visible light, each color represents a substance to be tested, so the microspheres can be used for simultaneous detection of various mycotoxins. Red, green and blue upconversion coded microspheres are separately linked with complete antigens of ochratoxin, zearalenone and aflatoxin to be used as models convenient for later detection experiments. However, in this method, the correspondence between colorcoding and mycotoxins is not unique, and the corresponding sequence can be changed according to the experimental needs. The flux, sensitive and rapid detection of various mycotoxins in the liquid phase environment is conducted by upconversion coded microspheres linked with mycotoxin complete antigens in combination with an indirect immune competitive reaction.

Description

technical field [0001] The invention relates to a preparation method of multi-color up-converting magnetically encoded polystyrene microspheres, which can realize rapid, accurate and quantitative detection of various mycotoxins at the same time. In particular, an upconversion magnetically encoded microsphere for the detection of mycotoxin flux and a preparation method thereof. Background technique [0002] Food safety is a major livelihood issue. In recent years, food safety problems caused by mycotoxins have become increasingly prominent, causing economic losses of tens of billions of dollars. Mycotoxins are secondary metabolites produced by fungi growing in food or feed. They are highly toxic and have the characteristics of trace amounts, strong toxicity, various types, and the coexistence of multiple toxins in the same food. Very difficult. [0003] At present, the national standards stipulate that the detection methods of mycotoxins in food include gas chromatography-m...

Claims

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Application Information

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IPC IPC(8): B01J13/02G01N33/569
CPCB01J13/02G01N33/56911
Inventor 王汉杰杨旻晔常津张莹崔梅慧
Owner TIANJIN UNIV
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