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Process and system for industrially producing glycosylglycerol

A technology of glucoside and glycerol, which is applied in the direction of supporting/immobilizing microorganisms, biochemical instruments, and sterilization methods, and can solve problems such as death, large harvesting membrane devices, and increased energy consumption for harvesting

Active Publication Date: 2018-12-07
青岛中科蓝智生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the algae mud harvested by this flat screen or inclined screen platform needs to flow into a recovery pond first, and it needs to use additional power such as piston pumps to transfer to algae mud dehydrator or other drying equipment or containers for further processing, and the process is long. , the recovery efficiency is low
[0004] In order to solve the practical problems of low harvesting efficiency and large footprint of flat screen or inclined screen device, Chinese patent application CN102696340A proposes a device for harvesting and dehydrating algae liquid with inclined drum filters connected in series, the core of which is Using the driving device of the drum, the multi-stage drum is driven to rotate and separate the algae liquid and algae mud, so that the pumped algae liquid rotates and moves in the drum filter
Compared with the existing unpowered flat sieve, it is necessary to use a high-power drive device to drive the heavy harvesting membrane platform to rotate. Because the harvesting membrane device is large and clumsy, the energy consumption of harvesting power is greatly increased, which is different from the currently commonly used unpowered screen. Compared with the multi-stage flat sieve, the technical advantage is not obvious, but the accumulation of algae mud on the surface of the drum filter membrane will cause the problem of poor harvesting
[0005] In addition, the current main means of extracting metabolites from microorganisms is to use 95% ethanol solution to infiltrate or extract by breaking the wall. Ethanol has good cell wall permeability and can well pass through the cell wall or dissolve the cell membrane, so that the microorganisms The metabolites are dissolved in ethanol, and the metabolites are separated through the rectification process in the later stage, but this extraction and separation technology will lead to the destruction of the internal tissue structure of the microorganisms and lead to death. The lifting of waste in the wall mode is also a burden of environmental governance
[0006] In summary, the existing microalgae cell harvesting, the extraction and purification of GG still have high harvesting costs and low efficiency, the microalgae cells cannot be recycled after extraction, and the separation and purification process of GG in the extract is currently still issues such as not being developed, therefore, it is necessary to study a new industrialized production system of GG

Method used

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  • Process and system for industrially producing glycosylglycerol
  • Process and system for industrially producing glycosylglycerol
  • Process and system for industrially producing glycosylglycerol

Examples

Experimental program
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Effect test

Embodiment 1

[0115] Such as Figure 1-5 , 7-8, a system for industrialized production of glycerol glucoside, including a cultivation system 1, a harvesting system 2 and an extraction system, the cultivation system 1, the harvesting system 2, and the extraction system are connected in sequence, and the cultivation system 1 is used for the growth of microalgae cells; the harvesting system 2 is used for separating the microalgae cells and culture fluid from the culture system, and sending the microalgae cells into the extraction system after separation.

[0116] The culture system includes: a photoreactor 101 , an inlet pipe 104 , a carbon-containing source gas 105 , an agitator 106 , an exhaust port 106 , and a light source 107 .

[0117] The cylindrical photoreactor 101 is filled with a mixture of microalgae cells 102 and culture solution 103, one end of the inlet pipe 104 is inserted into the culture solution 103, and the other end is connected to the gas source of the carbon source gas 10...

Embodiment 2

[0155] A system for industrialized production of glycerol glucoside is the same as that in Example 1, except that: (1) the bed surface of the filter residue bed 29 is made of a hard membrane material with membrane pores of 80 mesh.

[0156] (2) The bed surface of the recovery bed 30 is made of soft filter cloth with membrane holes of 100 mesh.

[0157] (3) The bed surface of the recovery bed 30 is divided into 4 gradients with respect to the inclination angle α gradient of the horizontal plane, followed by α 1 =5°, α 2 =15°, α 3 = 45°, α 4 =85°, such as Figure 6 shown.

[0158] (4) The uniform material port 16 is a combined device of a silo discharge port and a distribution plate. The silo discharge port injects the algae mud into the filter belt, and the accumulated algae mud is spread on the filter belt by the distribution plate.

[0159] (5) The filter belt 18 is made of a membrane material with a filter aperture of 1000 mesh.

Embodiment 3

[0161] A system for industrialized production of glycerol glucoside is the same as that in Example 1, except that: (1) the bed surface of the filter residue bed 29 is made of a hard membrane material with membrane pores of 30 mesh.

[0162] (2) The bed surface of the recovery bed 30 is made of soft filter cloth with membrane holes of 400 mesh.

[0163] (3) The material equalizing port 16 is a combined device of a silo discharge port and a distribution rod, the silo discharge port injects the algae mud into the filter belt, and the piled algae mud is spread on the filter belt by the distribution rod.

[0164] (4) The filter belt 18 is made of a membrane material with a filter aperture of 600 mesh.

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Abstract

The invention relates to a process and system for industrially producing glycosylglycerol, and belongs to the field of glucoside preparation. The system comprises a culture system, a collection system, an extraction system and a purification system which are connected in sequence. When a microalgae cell with high content of GG is cultured, a mixed solution of a metabolic product extracted from themicroalgae cell by utilizing the ecological characteristic of microalgae cell metabolic response mechanism while the microalgae cell activity and biology are relatively stable, and a mated device isdesigned, so that the efficiency for collecting and extracting the microalgae cell can be greatly improved, and the production cost for the metabolic product in the microalgae cell can be remarkably reduced. Finally, the invention further develops a process for separating and purifying GG from an extracting solution, and a whole set of process and system for industrially producing glycosylglycerol, which have an important practical significance for GG large-scale application.

Description

technical field [0001] The invention relates to the technical field of preparation of glycerol glucoside, in particular to a process and system for industrial production of glycerol glucoside. Background technique [0002] Microalgae is an autotrophic plant widely distributed in the natural environment. It can not only synthesize high value-added compounds such as oil, sugar, protein, and carotene through photosynthesis, but also because of its high photosynthetic efficiency, fast growth, and unit The yield per mu is high, and it is considered by the International Food and Agriculture Organization to be one of the most important food resources for human beings in the future. Glycerol glucoside (Glucosylglycerol, referred to as GG) is a kind of glycoside compound formed by linking glycerol molecules and glucose molecules through glycosidase reaction. In addition to being used as a flavor substance of food, glycerol glucoside can moisturize the skin, inhibit glucose metabolism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/00
CPCC12M47/02C12M47/04C12M47/10C12M47/12
Inventor 吕雪峰段仰凯张凯吴怀之刘祥
Owner 青岛中科蓝智生物科技发展有限公司
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