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Extraction method of protein in sludge and application of protein

An extraction method and protein technology, which are applied to the preparation methods of peptides, chemical instruments and methods, and the dissolution of microorganisms, etc., can solve the problems of high protein impurity content, unsuitable for nitrogen-containing fertilizers, and low protein yield, etc., and achieve simple operation. , The effect of realizing resource utilization and reducing the difficulty of extraction

Inactive Publication Date: 2018-12-07
SHENYANG AEROSPACE UNIVERSITY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of this, the present invention provides a protein extraction method and application in sludge to solve the problems of low protein yield and high impurity content in the extracted protein, which are not suitable for use as nitrogen-containing fertilizers using existing extraction methods

Method used

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  • Extraction method of protein in sludge and application of protein

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Effect test

Embodiment 1

[0043] First, 80g of sludge with a moisture content of 93% was ultrasonically used for 40min with an energy of 180W. Then, 0.04g / gTSS of papain was added thereto, and the speed of the constant temperature oscillator was adjusted to 160r / min, and the temperature was 60°C. Put the sludge into a constant temperature oscillator for hydrolysis for 5 hours, and inactivate it in boiling water for 15 minutes after the hydrolysis is completed. Then, the sludge was centrifuged at 10,000 g for 20 min, and the precipitate was discarded to obtain 60 ml of supernatant, which was the crude protein extract. The protein concentration was 133.3 mg / gTSS as detected by the Lowry method.

[0044] Concentrate 50ml of the above protein supernatant, adopt ammonium sulfate precipitation method coupled with dialysis bag adsorption method, first put the solution on a magnetic stirrer and stir slowly, slowly add 15g of ammonium sulfate solid into 50ml of protein supernatant, After stirring for 6 hours, l...

Embodiment 2

[0046] First, 80 g of sludge with a moisture content of 93% is ultrasonically 30 min, and the energy is 150 W, then papain of 0.03 g / gTSS is added therein, the rotating speed of the constant temperature oscillator is adjusted to be 150 r / min, and the temperature is 55 ° C, and the papain that has been added is The sludge was hydrolyzed in a constant temperature oscillator for 4 hours, and inactivated in boiling water for 15 minutes after the hydrolysis was completed. Afterwards, the sludge was centrifuged under 8000g centrifugal force for 15 minutes, and the precipitate was discarded to obtain 56ml supernatant, which was the crude protein extract. The protein concentration was 108.4mg / gTSS detected by the Lowry method.

[0047] Concentrate 50ml of the above protein supernatant, adopt ammonium sulfate precipitation method coupled with dialysis bag adsorption method, first put the solution on a magnetic stirrer and stir slowly, slowly add 10g of ammonium sulfate solid into 50ml o...

Embodiment 3

[0049] First, 80 g of sludge with a water content of 93% is ultrasonically 50 min, and the energy is 200 W, and then papain of 0.05 g / gTSS is added therein, and the rotating speed of the constant temperature oscillator is adjusted to 170 r / min, and the temperature is 65 ° C. The sludge was hydrolyzed in a constant temperature oscillator for 6 hours, and inactivated in boiling water for 15 minutes after the hydrolysis was completed. Afterwards, the sludge was centrifuged under 11000g centrifugal force for 25min, and the precipitate was discarded to obtain 63ml supernatant, which was the crude protein extract. The protein concentration was 136.3mg / gTSS detected by the Lowry method.

[0050] Concentrate the above 50ml protein supernatant, adopt ammonium sulfate precipitation method coupled with dialysis bag adsorption method, first put the solution on a magnetic stirrer and stir slowly, slowly add 20g ammonium sulfate solid into 60ml protein supernatant, After stirring for 7 hour...

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Abstract

The invention belongs to the fields of the treatment and recycling of solid wastes and particularly relates to an extraction method of a protein in sludge and application of the protein. The method comprises the following steps: carrying out combined treatment on sludge by virtue of ultrasonic waves and papain, so as to obtain a crude protein extracting solution; and purifying and concentrating the crude protein extracting solution through the coupling of an ammonium sulfate precipitation method and a dialysis bag adsorption method, so as to obtain a fine protein extracting solution. The extraction method has the beneficial effects that the whole operation flow is simple, the feasibility is high, other substances are not introduced, the further treatment and recycling of the sludge are notinfluenced, and the obtained protein fine extracting solution can be taken as a raw material for producing leaf fertilizers.

Description

technical field [0001] The invention belongs to the field of solid waste treatment and resource utilization, and particularly provides a protein extraction method and application in sludge. technical background [0002] With the acceleration of my country's urbanization process and the continuous improvement of sewage treatment rate, the output of sludge in urban sewage treatment plants has also increased significantly. A large amount of sludge is produced every year, and sludge is rich in organic matter, which is a potential of renewable resources. Studies have shown that protein, polysaccharides and some other carbohydrates are the main components of organic matter in sludge, and the content of protein accounts for 20% to 60% (relative to dry sludge). At present, the main domestic sludge disposal methods are composting, drying, incineration, landfill, and land utilization after dehydrating the sludge, which will not only waste the resources in the sludge, but also cause se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C07K1/14C07K1/30C07K1/36
CPCC07K1/145C07K1/30C07K1/36C12N1/06C12N1/066
Inventor 王伟云张进臧莹李润东
Owner SHENYANG AEROSPACE UNIVERSITY
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