Bacillus subtilis, application thereof, and enzyme preparation

A technology of Bacillus subtilis and enzyme preparations, applied in the direction of application, enzymes, bacteria, etc., to achieve the effects of shortening the process flow, assisting the treatment of high blood pressure, and stabilizing production quality

Active Publication Date: 2018-12-07
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no enzyme preparation specially used for h

Method used

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  • Bacillus subtilis, application thereof, and enzyme preparation
  • Bacillus subtilis, application thereof, and enzyme preparation
  • Bacillus subtilis, application thereof, and enzyme preparation

Examples

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[0032] Example 1

[0033] 1. Acquisition and identification of strain HU528

[0034] Soil was collected from a traditional Tibetan ghee workshop, 10 g of protein-rich soil samples were added to 100 mL of sterile water, and the bacterial suspension was prepared by shaking at 28 °C for 30 min. Culture on solid medium (0.4% (w / w, the same below) beef extract, 0.6% tryptone, 0.2% yeast extract, 0.5% NaCl, 2% agar, adjusted to pH 7.0) for 1-2 days. The single colonies with different growth traits obtained on the plate were inoculated into LB medium (1% tryptone, 0.5% yeast extract, 1% NaCl, adjusted to pH 7.0) for 48 hours, and the protease activity in the fermentation broth was measured to obtain protease-producing strains , named strain HU528.

[0035] After streaking the HU528 strain on solid plate medium at 37°C for 24h, the colony was white and round, with rough and wrinkled surfaces and irregular edges ( figure 1 ). Microscopic observation found that the strain was rod-sh...

Example Embodiment

[0052] Example 2

[0053] Prepare liquid medium as follows: add tryptone 2% (w / w, the same below), glucose 0.1%, CaCl to distilled water 2 0.2%, NaCl 0.1%, after mixing evenly, adjust the pH of the liquid medium to 5.0. After sterilization at 121°C for 20min, the first-class seeds of Bacillus subtilis HU528 were inoculated into the liquid medium at 1.0% (v / v), and fermented for 45h at a temperature of 30°C and a stirring speed of 150r / min. The obtained fermentation broth was centrifuged at 4° C. and 8000 r / m for 10 min to remove bacterial cells, and the fermentation supernatant was the enzyme preparation. The protease activity in the enzyme preparation was 800 U / mL, and the β-glucanase activity was 2.0 U / mL.

[0054] In the edible yeast powder, add the above-mentioned enzyme preparation (2.5mL enzyme preparation / g dry yeast) containing 2000U of protease activity per gram of yeast powder, and add sterile water to the volume ratio of edible yeast powder and mixed liquid (mater...

Example Embodiment

[0058] Example 3

[0059] The liquid medium was prepared as follows: in distilled water, add tryptone 1.5% (w / w, the same below), glucose 0.8%, CaCl 2 0.05%, NaCl 0.15%, after mixing evenly, adjust the pH of the liquid medium to 6.0. After sterilization at 121°C for 20min, the first-class seeds of Bacillus subtilis HU528 were inoculated into the liquid medium at 1.0% (v / v), and fermented for 60h at a temperature of 25°C and a stirring speed of 180r / min. , the obtained fermentation broth was centrifuged at 4° C. and 8000 r / m for 10 min to remove bacterial cells, and the fermentation supernatant was the enzyme preparation. The protease activity in the enzyme preparation was 1600 U / mL, and the β-glucanase activity was 3.0 U / mL.

[0060] In the edible yeast powder, add the above-mentioned enzyme preparation (6mL enzyme preparation / g dry yeast) containing 9600U of protease activity per gram of yeast powder, and add sterile water to the volume ratio of edible yeast powder and mixe...

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Abstract

The invention discloses a type of Bacillus subtilis, which is named HU 528, is preserved in Guangdong Culture Collection Center at Apr 23rd, 2018, and is assigned the accession number of GDMCC No.60364. The invention also discloses an enzyme preparation prepared from the Bacillus subtilis, and also discloses an application of preparing a yeast enzymolysis product, having blood pressure reducing activity, by the Bacillus subtilis. The enzyme preparation prepared from the Bacillus subtilis HU 528 has the activities of protease and beta-glucanase and can destruct yeast cell wall and hydrolyze yeast protein. The yeast enzymolysis product has excellent bioactivity; in-vivo and in-vitro activity tests prove that the enzymolysis product at least has blood pressure reducing and anti-oxidizing activity and can be used as a non-medicinal treatment food for hypertension patients. The product can effectively prevent, control, alleviate and auxiliarily treat the hypertension.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a bacillus subtilis and its application and an enzyme preparation. Background technique [0002] Non-drug treatment is an important means to treat and control hypertension. The antihypertensive polypeptide products developed with yeast as raw materials are expected to become safe and green functional foods for non-drug treatment of hypertension. Yeast powder was hydrolyzed with commercially available yeast extract compound enzyme, and the polypeptide obtained after removing sugar and nucleic acid substances had ACE inhibitory activity; after further application of ultrafiltration membrane separation and column chromatography, a hexapeptide with high blood pressure lowering activity was obtained . Mirzaei, a foreign scholar, prepared ACE inhibitory peptides by using ultrasonic wall breaking to assist trypsin hydrolysis of yeast. [0003] Since the yeast cell wall is thick, it i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12N9/54C12N1/06A23L33/14C12R1/125
CPCA23V2002/00A23L33/14C12N1/06C12N9/244C12N9/54C12Y302/01006C12N1/205C12R2001/125A23V2200/326A23V2250/76
Inventor 胡松青王佳佳侯轶
Owner SOUTH CHINA UNIV OF TECH
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